Experiment: PM_N2O with buyrate and 0.1 mM bicarb and 0.1 mM nitrate; in dark
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt TX73_019460 and TX73_019465 are separated by 20 nucleotides TX73_019465 and TX73_019470 overlap by 8 nucleotides
TX73_019460: TX73_019460 - 2-oxo-hepta-3-ene-1,7-dioic acid hydratase, at 4,253,886 to 4,254,692
_019460
TX73_019465: TX73_019465 - 5-carboxymethyl-2-hydroxymuconate Delta-isomerase, at 4,254,713 to 4,255,111
_019465
TX73_019470: TX73_019470 - 5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase, at 4,255,104 to 4,256,645
_019470
Position (kb) 4254
4255
4256 Strain fitness (log2 ratio) -1
0
1 at 4253.728 kb on - strand at 4253.758 kb on - strand at 4254.002 kb on + strand, within TX73_019460 at 4254.007 kb on + strand, within TX73_019460 at 4254.041 kb on + strand, within TX73_019460 at 4254.178 kb on + strand, within TX73_019460 at 4254.230 kb on - strand, within TX73_019460 at 4254.237 kb on - strand, within TX73_019460 at 4254.309 kb on + strand, within TX73_019460 at 4254.316 kb on + strand, within TX73_019460 at 4254.324 kb on - strand, within TX73_019460 at 4254.371 kb on - strand, within TX73_019460 at 4254.392 kb on + strand, within TX73_019460 at 4254.660 kb on + strand at 4254.828 kb on + strand, within TX73_019465 at 4254.838 kb on - strand, within TX73_019465 at 4254.951 kb on + strand, within TX73_019465 at 4254.953 kb on + strand, within TX73_019465 at 4254.984 kb on + strand, within TX73_019465 at 4255.021 kb on + strand, within TX73_019465 at 4255.092 kb on - strand at 4255.139 kb on + strand at 4255.175 kb on + strand at 4255.289 kb on + strand, within TX73_019470 at 4255.307 kb on - strand, within TX73_019470 at 4255.393 kb on - strand, within TX73_019470 at 4255.453 kb on - strand, within TX73_019470 at 4255.553 kb on - strand, within TX73_019470 at 4255.553 kb on - strand, within TX73_019470 at 4255.553 kb on - strand, within TX73_019470 at 4255.630 kb on - strand, within TX73_019470 at 4255.691 kb on + strand, within TX73_019470 at 4255.709 kb on - strand, within TX73_019470 at 4255.739 kb on + strand, within TX73_019470 at 4255.758 kb on + strand, within TX73_019470 at 4255.768 kb on - strand, within TX73_019470 at 4255.772 kb on + strand, within TX73_019470 at 4255.772 kb on + strand, within TX73_019470 at 4255.772 kb on + strand, within TX73_019470 at 4255.772 kb on + strand, within TX73_019470 at 4255.780 kb on - strand, within TX73_019470 at 4255.832 kb on - strand, within TX73_019470 at 4255.981 kb on + strand, within TX73_019470 at 4256.021 kb on - strand, within TX73_019470
Per-strain Table
Position Strand Gene LocusTag Fraction PM_N2O with buyrate and 0.1 mM bicarb and 0.1 mM nitrate; in dark remove 4,253,728 - -0.1 4,253,758 - +0.1 4,254,002 + TX73_019460 0.14 -0.5 4,254,007 + TX73_019460 0.15 -0.1 4,254,041 + TX73_019460 0.19 +0.4 4,254,178 + TX73_019460 0.36 +0.5 4,254,230 - TX73_019460 0.43 -0.1 4,254,237 - TX73_019460 0.43 -0.8 4,254,309 + TX73_019460 0.52 -0.2 4,254,316 + TX73_019460 0.53 +0.3 4,254,324 - TX73_019460 0.54 -0.6 4,254,371 - TX73_019460 0.60 -0.7 4,254,392 + TX73_019460 0.63 +0.8 4,254,660 + +0.1 4,254,828 + TX73_019465 0.29 -0.2 4,254,838 - TX73_019465 0.31 -0.3 4,254,951 + TX73_019465 0.60 +0.1 4,254,953 + TX73_019465 0.60 -0.1 4,254,984 + TX73_019465 0.68 +0.3 4,255,021 + TX73_019465 0.77 -0.4 4,255,092 - -1.5 4,255,139 + +0.9 4,255,175 + +0.1 4,255,289 + TX73_019470 0.12 +0.2 4,255,307 - TX73_019470 0.13 -0.5 4,255,393 - TX73_019470 0.19 +0.2 4,255,453 - TX73_019470 0.23 -0.4 4,255,553 - TX73_019470 0.29 -0.4 4,255,553 - TX73_019470 0.29 +0.0 4,255,553 - TX73_019470 0.29 -0.1 4,255,630 - TX73_019470 0.34 +0.1 4,255,691 + TX73_019470 0.38 +0.8 4,255,709 - TX73_019470 0.39 +0.1 4,255,739 + TX73_019470 0.41 +0.0 4,255,758 + TX73_019470 0.42 +0.4 4,255,768 - TX73_019470 0.43 -0.1 4,255,772 + TX73_019470 0.43 +0.3 4,255,772 + TX73_019470 0.43 -0.4 4,255,772 + TX73_019470 0.43 +0.0 4,255,772 + TX73_019470 0.43 +0.7 4,255,780 - TX73_019470 0.44 -1.7 4,255,832 - TX73_019470 0.47 -1.3 4,255,981 + TX73_019470 0.57 -0.2 4,256,021 - TX73_019470 0.59 -0.6
Or see this region's nucleotide sequence