Experiment: PM_N2O with buyrate and 0.1 mM bicarb and 0.1 mM nitrate; in dark
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt TX73_002990 and TX73_002995 are separated by 182 nucleotides TX73_002995 and TX73_003000 are separated by 95 nucleotides TX73_003000 and TX73_003005 are separated by 291 nucleotides
TX73_002990: TX73_002990 - transglycosylase domain-containing protein, at 640,107 to 642,326
_002990
TX73_002995: TX73_002995 - hypothetical protein, at 642,509 to 642,760
_002995
TX73_003000: TX73_003000 - hypothetical protein, at 642,856 to 643,068
_003000
TX73_003005: TX73_003005 - ABC transporter substrate-binding protein, at 643,360 to 644,580
_003005
Position (kb) 642
643
644 Strain fitness (log2 ratio) -2
-1
0
1
2 at 642.097 kb on + strand, within TX73_002990 at 642.099 kb on + strand, within TX73_002990 at 642.105 kb on - strand at 642.129 kb on - strand at 642.134 kb on - strand at 642.138 kb on + strand at 642.153 kb on - strand at 642.162 kb on - strand at 642.165 kb on + strand at 642.216 kb on - strand at 642.315 kb on + strand at 642.315 kb on + strand at 642.323 kb on - strand at 642.324 kb on - strand at 642.338 kb on + strand at 642.371 kb on + strand at 642.371 kb on + strand at 642.379 kb on - strand at 642.393 kb on + strand at 642.430 kb on + strand at 642.433 kb on - strand at 642.435 kb on + strand at 642.441 kb on + strand at 642.441 kb on + strand at 642.449 kb on - strand at 642.449 kb on - strand at 642.449 kb on - strand at 642.449 kb on - strand at 642.449 kb on - strand at 642.449 kb on - strand at 642.462 kb on - strand at 642.486 kb on + strand at 642.518 kb on + strand at 642.520 kb on + strand at 642.528 kb on - strand at 642.536 kb on - strand, within TX73_002995 at 642.546 kb on - strand, within TX73_002995 at 642.546 kb on - strand, within TX73_002995 at 642.546 kb on - strand, within TX73_002995 at 642.570 kb on - strand, within TX73_002995 at 642.597 kb on - strand, within TX73_002995 at 642.597 kb on - strand, within TX73_002995 at 642.603 kb on + strand, within TX73_002995 at 642.611 kb on - strand, within TX73_002995 at 642.614 kb on + strand, within TX73_002995 at 642.614 kb on + strand, within TX73_002995 at 642.629 kb on - strand, within TX73_002995 at 642.630 kb on + strand, within TX73_002995 at 642.666 kb on + strand, within TX73_002995 at 642.768 kb on + strand at 642.768 kb on + strand at 642.776 kb on - strand at 642.786 kb on + strand at 642.882 kb on - strand, within TX73_003000 at 642.916 kb on - strand, within TX73_003000 at 642.945 kb on + strand, within TX73_003000 at 642.952 kb on - strand, within TX73_003000 at 642.990 kb on + strand, within TX73_003000 at 643.015 kb on + strand, within TX73_003000 at 643.032 kb on + strand, within TX73_003000 at 643.047 kb on + strand at 643.065 kb on - strand at 643.264 kb on - strand at 643.271 kb on - strand at 643.332 kb on - strand at 643.406 kb on - strand at 643.457 kb on + strand at 643.474 kb on - strand at 643.516 kb on - strand, within TX73_003005 at 643.587 kb on + strand, within TX73_003005 at 643.595 kb on - strand, within TX73_003005 at 643.772 kb on - strand, within TX73_003005 at 643.836 kb on - strand, within TX73_003005 at 643.874 kb on + strand, within TX73_003005 at 643.877 kb on + strand, within TX73_003005 at 643.877 kb on + strand, within TX73_003005 at 643.885 kb on - strand, within TX73_003005 at 643.995 kb on - strand, within TX73_003005 at 644.005 kb on - strand, within TX73_003005
Per-strain Table
Position Strand Gene LocusTag Fraction PM_N2O with buyrate and 0.1 mM bicarb and 0.1 mM nitrate; in dark remove 642,097 + TX73_002990 0.90 +0.1 642,099 + TX73_002990 0.90 -0.7 642,105 - +0.8 642,129 - +2.2 642,134 - +2.6 642,138 + -0.3 642,153 - +2.0 642,162 - -0.2 642,165 + -0.5 642,216 - -0.0 642,315 + +0.1 642,315 + +0.4 642,323 - +1.2 642,324 - -0.3 642,338 + -0.1 642,371 + -0.2 642,371 + +0.2 642,379 - +0.5 642,393 + +0.0 642,430 + -1.0 642,433 - -0.6 642,435 + -0.0 642,441 + +0.1 642,441 + -0.3 642,449 - +0.2 642,449 - -1.1 642,449 - -0.5 642,449 - -0.8 642,449 - -1.0 642,449 - +0.0 642,462 - +0.1 642,486 + -0.4 642,518 + +0.1 642,520 + +1.2 642,528 - -0.3 642,536 - TX73_002995 0.11 -0.2 642,546 - TX73_002995 0.15 +1.0 642,546 - TX73_002995 0.15 +0.1 642,546 - TX73_002995 0.15 +0.6 642,570 - TX73_002995 0.24 +0.1 642,597 - TX73_002995 0.35 +0.2 642,597 - TX73_002995 0.35 -0.7 642,603 + TX73_002995 0.37 +0.4 642,611 - TX73_002995 0.40 +0.9 642,614 + TX73_002995 0.42 +1.0 642,614 + TX73_002995 0.42 +0.5 642,629 - TX73_002995 0.48 -0.8 642,630 + TX73_002995 0.48 +0.7 642,666 + TX73_002995 0.62 +0.9 642,768 + +0.4 642,768 + -0.2 642,776 - +0.6 642,786 + -0.2 642,882 - TX73_003000 0.12 +0.1 642,916 - TX73_003000 0.28 +0.5 642,945 + TX73_003000 0.42 +2.2 642,952 - TX73_003000 0.45 -0.6 642,990 + TX73_003000 0.63 -0.7 643,015 + TX73_003000 0.75 -1.8 643,032 + TX73_003000 0.83 -1.8 643,047 + +0.0 643,065 - -0.2 643,264 - -0.0 643,271 - +0.0 643,332 - +1.9 643,406 - -2.1 643,457 + -0.4 643,474 - -0.1 643,516 - TX73_003005 0.13 +0.1 643,587 + TX73_003005 0.19 -0.2 643,595 - TX73_003005 0.19 +0.2 643,772 - TX73_003005 0.34 +0.8 643,836 - TX73_003005 0.39 -0.3 643,874 + TX73_003005 0.42 -0.9 643,877 + TX73_003005 0.42 +0.1 643,877 + TX73_003005 0.42 +0.7 643,885 - TX73_003005 0.43 -0.4 643,995 - TX73_003005 0.52 -0.2 644,005 - TX73_003005 0.53 +0.1
Or see this region's nucleotide sequence