Experiment: mutant library grown in Supernatant; Rhizobium pusense TBS_038 grown in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_00068 and MPMX20_00069 are separated by 53 nucleotides MPMX20_00069 and MPMX20_00070 are separated by 130 nucleotides MPMX20_00070 and MPMX20_00071 are separated by 147 nucleotides
MPMX20_00068: MPMX20_00068 - HTH-type transcriptional regulator CdhR, at 69,240 to 70,193
_00068
MPMX20_00069: MPMX20_00069 - hypothetical protein, at 70,247 to 70,663
_00069
MPMX20_00070: MPMX20_00070 - hypothetical protein, at 70,794 to 71,882
_00070
MPMX20_00071: MPMX20_00071 - hypothetical protein, at 72,030 to 72,989
_00071
Position (kb) 70
71
72 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 69.820 kb on - strand, within MPMX20_00068 at 69.891 kb on + strand, within MPMX20_00068 at 69.903 kb on - strand, within MPMX20_00068 at 69.938 kb on + strand, within MPMX20_00068 at 69.944 kb on + strand, within MPMX20_00068 at 69.956 kb on - strand, within MPMX20_00068 at 70.050 kb on - strand, within MPMX20_00068 at 70.063 kb on + strand, within MPMX20_00068 at 70.084 kb on + strand, within MPMX20_00068 at 70.098 kb on + strand at 70.100 kb on + strand at 70.100 kb on + strand at 70.100 kb on + strand at 70.106 kb on - strand at 70.106 kb on - strand at 70.107 kb on - strand at 70.108 kb on - strand at 70.108 kb on - strand at 70.110 kb on + strand at 70.110 kb on + strand at 70.118 kb on - strand at 70.118 kb on - strand at 70.118 kb on - strand at 70.159 kb on - strand at 70.166 kb on + strand at 70.166 kb on + strand at 70.166 kb on + strand at 70.166 kb on + strand at 70.174 kb on - strand at 70.182 kb on - strand at 70.186 kb on + strand at 70.210 kb on + strand at 70.230 kb on + strand at 70.259 kb on - strand at 70.270 kb on - strand at 70.275 kb on + strand at 70.290 kb on + strand, within MPMX20_00069 at 70.298 kb on - strand, within MPMX20_00069 at 70.386 kb on + strand, within MPMX20_00069 at 70.407 kb on + strand, within MPMX20_00069 at 70.407 kb on + strand, within MPMX20_00069 at 70.407 kb on + strand, within MPMX20_00069 at 70.416 kb on + strand, within MPMX20_00069 at 70.416 kb on + strand, within MPMX20_00069 at 70.416 kb on + strand, within MPMX20_00069 at 70.424 kb on - strand, within MPMX20_00069 at 70.424 kb on - strand, within MPMX20_00069 at 70.425 kb on + strand, within MPMX20_00069 at 70.425 kb on + strand, within MPMX20_00069 at 70.434 kb on - strand, within MPMX20_00069 at 70.446 kb on + strand, within MPMX20_00069 at 70.448 kb on + strand, within MPMX20_00069 at 70.448 kb on + strand, within MPMX20_00069 at 70.448 kb on + strand, within MPMX20_00069 at 70.448 kb on + strand, within MPMX20_00069 at 70.448 kb on + strand, within MPMX20_00069 at 70.448 kb on + strand, within MPMX20_00069 at 70.448 kb on + strand, within MPMX20_00069 at 70.448 kb on + strand, within MPMX20_00069 at 70.448 kb on + strand, within MPMX20_00069 at 70.448 kb on + strand, within MPMX20_00069 at 70.448 kb on + strand, within MPMX20_00069 at 70.448 kb on + strand, within MPMX20_00069 at 70.448 kb on + strand, within MPMX20_00069 at 70.448 kb on + strand, within MPMX20_00069 at 70.448 kb on + strand, within MPMX20_00069 at 70.448 kb on + strand, within MPMX20_00069 at 70.448 kb on + strand, within MPMX20_00069 at 70.456 kb on - strand, within MPMX20_00069 at 70.456 kb on - strand, within MPMX20_00069 at 70.456 kb on - strand, within MPMX20_00069 at 70.456 kb on - strand, within MPMX20_00069 at 70.456 kb on - strand, within MPMX20_00069 at 70.472 kb on - strand, within MPMX20_00069 at 70.472 kb on - strand, within MPMX20_00069 at 70.480 kb on + strand, within MPMX20_00069 at 70.482 kb on + strand, within MPMX20_00069 at 70.494 kb on + strand, within MPMX20_00069 at 70.498 kb on + strand, within MPMX20_00069 at 70.502 kb on - strand, within MPMX20_00069 at 70.576 kb on - strand, within MPMX20_00069 at 70.578 kb on + strand, within MPMX20_00069 at 70.615 kb on + strand, within MPMX20_00069 at 70.675 kb on + strand at 70.682 kb on + strand at 70.683 kb on + strand at 70.690 kb on + strand at 70.690 kb on + strand at 70.697 kb on - strand at 70.698 kb on - strand at 70.716 kb on - strand at 70.729 kb on - strand at 70.730 kb on + strand at 70.731 kb on + strand at 70.739 kb on - strand at 70.739 kb on - strand at 70.769 kb on + strand at 70.782 kb on - strand at 70.791 kb on + strand at 70.791 kb on - strand at 70.793 kb on - strand at 70.796 kb on + strand at 70.796 kb on + strand at 70.796 kb on + strand at 70.796 kb on + strand at 70.799 kb on - strand at 70.799 kb on - strand at 70.799 kb on - strand at 70.804 kb on - strand at 70.804 kb on - strand at 70.804 kb on - strand at 70.804 kb on - strand at 70.814 kb on - strand at 70.814 kb on - strand at 70.818 kb on - strand at 70.818 kb on - strand at 70.828 kb on + strand at 70.891 kb on - strand at 71.041 kb on - strand, within MPMX20_00070 at 71.091 kb on + strand, within MPMX20_00070 at 71.091 kb on + strand, within MPMX20_00070 at 71.091 kb on + strand, within MPMX20_00070 at 71.094 kb on - strand, within MPMX20_00070 at 71.099 kb on - strand, within MPMX20_00070 at 71.099 kb on - strand, within MPMX20_00070 at 71.179 kb on - strand, within MPMX20_00070 at 71.179 kb on - strand, within MPMX20_00070 at 71.237 kb on + strand, within MPMX20_00070 at 71.266 kb on - strand, within MPMX20_00070 at 71.266 kb on - strand, within MPMX20_00070 at 71.302 kb on - strand, within MPMX20_00070 at 71.376 kb on + strand, within MPMX20_00070 at 71.438 kb on - strand, within MPMX20_00070 at 71.506 kb on - strand, within MPMX20_00070 at 71.575 kb on + strand, within MPMX20_00070 at 71.575 kb on + strand, within MPMX20_00070 at 71.575 kb on + strand, within MPMX20_00070 at 71.575 kb on + strand, within MPMX20_00070 at 71.575 kb on + strand, within MPMX20_00070 at 71.575 kb on + strand, within MPMX20_00070 at 71.583 kb on - strand, within MPMX20_00070 at 71.665 kb on + strand, within MPMX20_00070 at 71.670 kb on + strand, within MPMX20_00070 at 71.670 kb on + strand, within MPMX20_00070 at 71.670 kb on + strand, within MPMX20_00070 at 71.670 kb on + strand, within MPMX20_00070 at 71.678 kb on - strand, within MPMX20_00070 at 71.680 kb on + strand, within MPMX20_00070 at 71.680 kb on + strand, within MPMX20_00070 at 71.856 kb on - strand at 71.959 kb on + strand at 71.979 kb on + strand at 71.979 kb on + strand at 71.981 kb on + strand at 71.989 kb on - strand at 71.994 kb on - strand at 72.002 kb on + strand at 72.005 kb on - strand at 72.006 kb on + strand at 72.038 kb on - strand at 72.052 kb on - strand at 72.064 kb on + strand at 72.123 kb on + strand at 72.132 kb on - strand, within MPMX20_00071 at 72.211 kb on + strand, within MPMX20_00071 at 72.221 kb on - strand, within MPMX20_00071 at 72.259 kb on + strand, within MPMX20_00071 at 72.266 kb on - strand, within MPMX20_00071 at 72.295 kb on + strand, within MPMX20_00071 at 72.353 kb on + strand, within MPMX20_00071 at 72.415 kb on - strand, within MPMX20_00071 at 72.447 kb on + strand, within MPMX20_00071 at 72.450 kb on + strand, within MPMX20_00071 at 72.458 kb on - strand, within MPMX20_00071 at 72.488 kb on - strand, within MPMX20_00071 at 72.536 kb on + strand, within MPMX20_00071 at 72.571 kb on + strand, within MPMX20_00071 at 72.571 kb on + strand, within MPMX20_00071 at 72.579 kb on - strand, within MPMX20_00071 at 72.579 kb on - strand, within MPMX20_00071 at 72.579 kb on - strand, within MPMX20_00071 at 72.581 kb on + strand, within MPMX20_00071 at 72.589 kb on - strand, within MPMX20_00071 at 72.604 kb on - strand, within MPMX20_00071 at 72.608 kb on + strand, within MPMX20_00071 at 72.785 kb on + strand, within MPMX20_00071 at 72.814 kb on - strand, within MPMX20_00071 at 72.826 kb on - strand, within MPMX20_00071 at 72.840 kb on + strand, within MPMX20_00071 at 72.840 kb on + strand, within MPMX20_00071 at 72.851 kb on + strand, within MPMX20_00071 at 72.859 kb on - strand, within MPMX20_00071 at 72.859 kb on - strand, within MPMX20_00071 at 72.859 kb on - strand, within MPMX20_00071 at 72.859 kb on - strand, within MPMX20_00071 at 72.859 kb on - strand, within MPMX20_00071 at 72.880 kb on + strand, within MPMX20_00071
Per-strain Table
Position Strand Gene LocusTag Fraction mutant library grown in Supernatant; Rhizobium pusense TBS_038 grown in LB remove 69,820 - MPMX20_00068 0.61 +0.2 69,891 + MPMX20_00068 0.68 -0.1 69,903 - MPMX20_00068 0.69 -0.2 69,938 + MPMX20_00068 0.73 +1.0 69,944 + MPMX20_00068 0.74 -0.2 69,956 - MPMX20_00068 0.75 +0.2 70,050 - MPMX20_00068 0.85 +0.3 70,063 + MPMX20_00068 0.86 -0.6 70,084 + MPMX20_00068 0.88 +0.1 70,098 + +0.8 70,100 + -0.2 70,100 + +0.2 70,100 + +0.3 70,106 - +0.3 70,106 - +0.0 70,107 - +0.4 70,108 - +0.2 70,108 - +0.8 70,110 + -0.8 70,110 + +0.2 70,118 - -0.0 70,118 - -0.2 70,118 - +1.2 70,159 - +0.0 70,166 + -0.6 70,166 + -0.2 70,166 + +2.3 70,166 + -0.1 70,174 - -1.5 70,182 - -0.2 70,186 + +0.3 70,210 + +0.4 70,230 + -0.5 70,259 - +0.8 70,270 - -0.5 70,275 + +0.3 70,290 + MPMX20_00069 0.10 +0.0 70,298 - MPMX20_00069 0.12 -0.1 70,386 + MPMX20_00069 0.33 +0.2 70,407 + MPMX20_00069 0.38 +0.0 70,407 + MPMX20_00069 0.38 +0.1 70,407 + MPMX20_00069 0.38 +0.8 70,416 + MPMX20_00069 0.41 +0.3 70,416 + MPMX20_00069 0.41 +0.3 70,416 + MPMX20_00069 0.41 +1.0 70,424 - MPMX20_00069 0.42 -0.4 70,424 - MPMX20_00069 0.42 -0.7 70,425 + MPMX20_00069 0.43 +0.6 70,425 + MPMX20_00069 0.43 -0.6 70,434 - MPMX20_00069 0.45 -0.2 70,446 + MPMX20_00069 0.48 +0.5 70,448 + MPMX20_00069 0.48 +0.0 70,448 + MPMX20_00069 0.48 -0.3 70,448 + MPMX20_00069 0.48 +0.5 70,448 + MPMX20_00069 0.48 -0.0 70,448 + MPMX20_00069 0.48 -0.1 70,448 + MPMX20_00069 0.48 +0.3 70,448 + MPMX20_00069 0.48 +1.3 70,448 + MPMX20_00069 0.48 +0.4 70,448 + MPMX20_00069 0.48 -0.1 70,448 + MPMX20_00069 0.48 -0.4 70,448 + MPMX20_00069 0.48 +0.8 70,448 + MPMX20_00069 0.48 +0.2 70,448 + MPMX20_00069 0.48 -0.5 70,448 + MPMX20_00069 0.48 -0.0 70,448 + MPMX20_00069 0.48 -0.5 70,448 + MPMX20_00069 0.48 +0.3 70,448 + MPMX20_00069 0.48 -0.4 70,456 - MPMX20_00069 0.50 +0.5 70,456 - MPMX20_00069 0.50 -0.2 70,456 - MPMX20_00069 0.50 -0.2 70,456 - MPMX20_00069 0.50 +0.5 70,456 - MPMX20_00069 0.50 -0.9 70,472 - MPMX20_00069 0.54 -0.0 70,472 - MPMX20_00069 0.54 +0.0 70,480 + MPMX20_00069 0.56 +0.8 70,482 + MPMX20_00069 0.56 +0.9 70,494 + MPMX20_00069 0.59 -0.7 70,498 + MPMX20_00069 0.60 +0.2 70,502 - MPMX20_00069 0.61 +0.5 70,576 - MPMX20_00069 0.79 +0.0 70,578 + MPMX20_00069 0.79 +0.6 70,615 + MPMX20_00069 0.88 +0.1 70,675 + +0.3 70,682 + -1.6 70,683 + -2.1 70,690 + +2.0 70,690 + +0.4 70,697 - +4.1 70,698 - -0.1 70,716 - +0.3 70,729 - +0.1 70,730 + +0.5 70,731 + +0.8 70,739 - +0.3 70,739 - -0.6 70,769 + +0.4 70,782 - +1.8 70,791 + -1.1 70,791 - -0.6 70,793 - -0.2 70,796 + +0.8 70,796 + -0.4 70,796 + +1.0 70,796 + +0.4 70,799 - -0.7 70,799 - -0.7 70,799 - -0.0 70,804 - +0.1 70,804 - -0.3 70,804 - -0.6 70,804 - -0.0 70,814 - -0.0 70,814 - -1.2 70,818 - -0.2 70,818 - -0.0 70,828 + +0.3 70,891 - +0.1 71,041 - MPMX20_00070 0.23 -0.8 71,091 + MPMX20_00070 0.27 -0.3 71,091 + MPMX20_00070 0.27 -0.1 71,091 + MPMX20_00070 0.27 +0.3 71,094 - MPMX20_00070 0.28 -1.2 71,099 - MPMX20_00070 0.28 +0.1 71,099 - MPMX20_00070 0.28 +0.1 71,179 - MPMX20_00070 0.35 -0.2 71,179 - MPMX20_00070 0.35 +0.3 71,237 + MPMX20_00070 0.41 +0.0 71,266 - MPMX20_00070 0.43 -0.2 71,266 - MPMX20_00070 0.43 +0.6 71,302 - MPMX20_00070 0.47 +0.4 71,376 + MPMX20_00070 0.53 -0.3 71,438 - MPMX20_00070 0.59 -0.2 71,506 - MPMX20_00070 0.65 +0.2 71,575 + MPMX20_00070 0.72 +0.4 71,575 + MPMX20_00070 0.72 -0.5 71,575 + MPMX20_00070 0.72 -0.0 71,575 + MPMX20_00070 0.72 +2.3 71,575 + MPMX20_00070 0.72 -0.8 71,575 + MPMX20_00070 0.72 +0.2 71,583 - MPMX20_00070 0.72 +0.7 71,665 + MPMX20_00070 0.80 -0.9 71,670 + MPMX20_00070 0.80 +0.3 71,670 + MPMX20_00070 0.80 +0.1 71,670 + MPMX20_00070 0.80 +0.6 71,670 + MPMX20_00070 0.80 -0.0 71,678 - MPMX20_00070 0.81 -0.6 71,680 + MPMX20_00070 0.81 +0.7 71,680 + MPMX20_00070 0.81 +0.0 71,856 - -0.0 71,959 + -0.7 71,979 + +0.4 71,979 + -0.3 71,981 + +0.3 71,989 - -0.4 71,994 - -0.4 72,002 + -0.9 72,005 - +0.1 72,006 + +0.6 72,038 - +0.4 72,052 - -0.3 72,064 + +0.1 72,123 + -0.5 72,132 - MPMX20_00071 0.11 -1.5 72,211 + MPMX20_00071 0.19 +1.6 72,221 - MPMX20_00071 0.20 -0.4 72,259 + MPMX20_00071 0.24 +0.6 72,266 - MPMX20_00071 0.25 +0.1 72,295 + MPMX20_00071 0.28 -0.3 72,353 + MPMX20_00071 0.34 +0.8 72,415 - MPMX20_00071 0.40 -0.0 72,447 + MPMX20_00071 0.43 +0.5 72,450 + MPMX20_00071 0.44 -0.1 72,458 - MPMX20_00071 0.45 -0.5 72,488 - MPMX20_00071 0.48 -0.4 72,536 + MPMX20_00071 0.53 +0.4 72,571 + MPMX20_00071 0.56 +0.0 72,571 + MPMX20_00071 0.56 +0.0 72,579 - MPMX20_00071 0.57 -0.0 72,579 - MPMX20_00071 0.57 +0.5 72,579 - MPMX20_00071 0.57 +0.1 72,581 + MPMX20_00071 0.57 -0.3 72,589 - MPMX20_00071 0.58 -0.4 72,604 - MPMX20_00071 0.60 +0.2 72,608 + MPMX20_00071 0.60 +0.5 72,785 + MPMX20_00071 0.79 -0.2 72,814 - MPMX20_00071 0.82 +0.4 72,826 - MPMX20_00071 0.83 +1.3 72,840 + MPMX20_00071 0.84 +0.1 72,840 + MPMX20_00071 0.84 +0.4 72,851 + MPMX20_00071 0.86 +0.8 72,859 - MPMX20_00071 0.86 -0.0 72,859 - MPMX20_00071 0.86 -0.3 72,859 - MPMX20_00071 0.86 +0.9 72,859 - MPMX20_00071 0.86 +0.0 72,859 - MPMX20_00071 0.86 +0.3 72,880 + MPMX20_00071 0.89 +0.1
Or see this region's nucleotide sequence