Experiment: mutant library grown in Supernatant; Paenibacillus favisporus 004-C2 grown in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_04684 and MPMX20_04685 are separated by 301 nucleotides MPMX20_04685 and MPMX20_04686 are separated by 131 nucleotides MPMX20_04686 and MPMX20_04687 are separated by 331 nucleotides MPMX20_04687 and MPMX20_04688 are separated by 41 nucleotides
MPMX20_04684: MPMX20_04684 - hypothetical protein, at 37,873 to 38,736
_04684
MPMX20_04685: MPMX20_04685 - hypothetical protein, at 39,038 to 39,280
_04685
MPMX20_04686: MPMX20_04686 - Phospholipase D, at 39,412 to 39,792
_04686
MPMX20_04687: MPMX20_04687 - hypothetical protein, at 40,124 to 40,465
_04687
MPMX20_04688: MPMX20_04688 - hypothetical protein, at 40,507 to 41,238
_04688
Position (kb) 39
40 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2
3 at 38.450 kb on - strand, within MPMX20_04684 at 38.455 kb on + strand, within MPMX20_04684 at 38.478 kb on + strand, within MPMX20_04684 at 38.498 kb on + strand, within MPMX20_04684 at 38.508 kb on - strand, within MPMX20_04684 at 38.527 kb on + strand, within MPMX20_04684 at 38.552 kb on + strand, within MPMX20_04684 at 38.552 kb on + strand, within MPMX20_04684 at 38.552 kb on + strand, within MPMX20_04684 at 38.560 kb on - strand, within MPMX20_04684 at 38.569 kb on + strand, within MPMX20_04684 at 38.605 kb on + strand, within MPMX20_04684 at 38.631 kb on + strand, within MPMX20_04684 at 38.631 kb on + strand, within MPMX20_04684 at 38.631 kb on + strand, within MPMX20_04684 at 38.631 kb on + strand, within MPMX20_04684 at 38.631 kb on + strand, within MPMX20_04684 at 38.631 kb on + strand, within MPMX20_04684 at 38.631 kb on + strand, within MPMX20_04684 at 38.639 kb on - strand, within MPMX20_04684 at 38.639 kb on - strand, within MPMX20_04684 at 38.639 kb on - strand, within MPMX20_04684 at 38.639 kb on - strand, within MPMX20_04684 at 38.639 kb on - strand, within MPMX20_04684 at 38.661 kb on - strand at 38.661 kb on - strand at 38.730 kb on + strand at 38.810 kb on + strand at 38.810 kb on + strand at 38.816 kb on - strand at 38.816 kb on - strand at 38.830 kb on - strand at 38.830 kb on - strand at 38.836 kb on + strand at 38.844 kb on - strand at 38.846 kb on - strand at 38.853 kb on - strand at 38.853 kb on - strand at 38.867 kb on - strand at 38.964 kb on - strand at 38.967 kb on + strand at 39.057 kb on + strand at 39.070 kb on + strand, within MPMX20_04685 at 39.070 kb on + strand, within MPMX20_04685 at 39.070 kb on + strand, within MPMX20_04685 at 39.070 kb on + strand, within MPMX20_04685 at 39.070 kb on + strand, within MPMX20_04685 at 39.070 kb on + strand, within MPMX20_04685 at 39.070 kb on + strand, within MPMX20_04685 at 39.070 kb on + strand, within MPMX20_04685 at 39.070 kb on + strand, within MPMX20_04685 at 39.078 kb on - strand, within MPMX20_04685 at 39.078 kb on - strand, within MPMX20_04685 at 39.078 kb on - strand, within MPMX20_04685 at 39.101 kb on - strand, within MPMX20_04685 at 39.119 kb on + strand, within MPMX20_04685 at 39.124 kb on - strand, within MPMX20_04685 at 39.205 kb on + strand, within MPMX20_04685 at 39.213 kb on - strand, within MPMX20_04685 at 39.213 kb on - strand, within MPMX20_04685 at 39.213 kb on - strand, within MPMX20_04685 at 39.219 kb on + strand, within MPMX20_04685 at 39.219 kb on + strand, within MPMX20_04685 at 39.219 kb on + strand, within MPMX20_04685 at 39.219 kb on + strand, within MPMX20_04685 at 39.227 kb on - strand, within MPMX20_04685 at 39.289 kb on + strand at 39.289 kb on + strand at 39.289 kb on + strand at 39.295 kb on + strand at 39.320 kb on + strand at 39.328 kb on - strand at 39.377 kb on - strand at 39.377 kb on - strand at 39.383 kb on - strand at 39.478 kb on + strand, within MPMX20_04686 at 39.478 kb on + strand, within MPMX20_04686 at 39.478 kb on + strand, within MPMX20_04686 at 39.478 kb on + strand, within MPMX20_04686 at 39.478 kb on + strand, within MPMX20_04686 at 39.478 kb on + strand, within MPMX20_04686 at 39.478 kb on + strand, within MPMX20_04686 at 39.478 kb on + strand, within MPMX20_04686 at 39.478 kb on + strand, within MPMX20_04686 at 39.478 kb on + strand, within MPMX20_04686 at 39.514 kb on + strand, within MPMX20_04686 at 39.546 kb on + strand, within MPMX20_04686 at 39.561 kb on + strand, within MPMX20_04686 at 39.622 kb on - strand, within MPMX20_04686 at 39.622 kb on - strand, within MPMX20_04686 at 39.630 kb on + strand, within MPMX20_04686 at 39.663 kb on - strand, within MPMX20_04686 at 39.687 kb on + strand, within MPMX20_04686 at 39.692 kb on + strand, within MPMX20_04686 at 39.695 kb on - strand, within MPMX20_04686 at 39.716 kb on + strand, within MPMX20_04686 at 39.719 kb on - strand, within MPMX20_04686 at 39.719 kb on - strand, within MPMX20_04686 at 39.723 kb on + strand, within MPMX20_04686 at 39.723 kb on + strand, within MPMX20_04686 at 39.724 kb on - strand, within MPMX20_04686 at 39.724 kb on - strand, within MPMX20_04686 at 39.724 kb on - strand, within MPMX20_04686 at 39.724 kb on - strand, within MPMX20_04686 at 39.731 kb on - strand, within MPMX20_04686 at 39.760 kb on + strand at 39.765 kb on + strand at 39.765 kb on + strand at 39.768 kb on - strand at 39.768 kb on - strand at 39.781 kb on + strand at 39.782 kb on + strand at 39.790 kb on - strand at 39.796 kb on - strand at 39.807 kb on + strand at 39.807 kb on - strand at 39.832 kb on - strand at 39.835 kb on + strand at 39.843 kb on + strand at 39.843 kb on + strand at 39.843 kb on + strand at 39.843 kb on + strand at 39.845 kb on + strand at 39.851 kb on - strand at 39.851 kb on - strand at 39.851 kb on - strand at 39.892 kb on - strand at 39.892 kb on - strand at 39.905 kb on - strand at 39.912 kb on + strand at 39.912 kb on + strand at 39.912 kb on + strand at 39.912 kb on + strand at 39.912 kb on + strand at 39.912 kb on + strand at 39.912 kb on + strand at 39.917 kb on + strand at 39.920 kb on - strand at 39.920 kb on - strand at 39.920 kb on - strand at 39.921 kb on - strand at 39.939 kb on - strand at 39.962 kb on + strand at 40.005 kb on - strand at 40.014 kb on - strand at 40.045 kb on + strand at 40.050 kb on + strand at 40.058 kb on - strand at 40.058 kb on - strand at 40.058 kb on - strand at 40.063 kb on + strand at 40.063 kb on + strand at 40.072 kb on + strand at 40.089 kb on + strand at 40.094 kb on + strand at 40.102 kb on + strand at 40.102 kb on - strand at 40.131 kb on - strand at 40.131 kb on - strand at 40.136 kb on - strand at 40.146 kb on - strand at 40.152 kb on + strand at 40.152 kb on + strand at 40.168 kb on + strand, within MPMX20_04687 at 40.168 kb on + strand, within MPMX20_04687 at 40.197 kb on - strand, within MPMX20_04687 at 40.211 kb on - strand, within MPMX20_04687 at 40.223 kb on - strand, within MPMX20_04687 at 40.241 kb on + strand, within MPMX20_04687 at 40.243 kb on - strand, within MPMX20_04687 at 40.288 kb on - strand, within MPMX20_04687 at 40.307 kb on + strand, within MPMX20_04687 at 40.308 kb on + strand, within MPMX20_04687 at 40.308 kb on + strand, within MPMX20_04687 at 40.308 kb on + strand, within MPMX20_04687 at 40.311 kb on - strand, within MPMX20_04687 at 40.316 kb on - strand, within MPMX20_04687 at 40.316 kb on - strand, within MPMX20_04687 at 40.316 kb on - strand, within MPMX20_04687 at 40.316 kb on - strand, within MPMX20_04687 at 40.316 kb on - strand, within MPMX20_04687 at 40.341 kb on + strand, within MPMX20_04687 at 40.349 kb on + strand, within MPMX20_04687 at 40.349 kb on - strand, within MPMX20_04687 at 40.360 kb on + strand, within MPMX20_04687 at 40.360 kb on + strand, within MPMX20_04687 at 40.368 kb on - strand, within MPMX20_04687 at 40.368 kb on - strand, within MPMX20_04687 at 40.370 kb on + strand, within MPMX20_04687 at 40.370 kb on + strand, within MPMX20_04687 at 40.370 kb on + strand, within MPMX20_04687 at 40.370 kb on + strand, within MPMX20_04687 at 40.370 kb on + strand, within MPMX20_04687 at 40.370 kb on + strand, within MPMX20_04687 at 40.370 kb on + strand, within MPMX20_04687 at 40.370 kb on + strand, within MPMX20_04687 at 40.370 kb on + strand, within MPMX20_04687 at 40.375 kb on + strand, within MPMX20_04687 at 40.375 kb on + strand, within MPMX20_04687 at 40.375 kb on + strand, within MPMX20_04687 at 40.375 kb on + strand, within MPMX20_04687 at 40.378 kb on - strand, within MPMX20_04687 at 40.378 kb on - strand, within MPMX20_04687 at 40.378 kb on - strand, within MPMX20_04687 at 40.378 kb on - strand, within MPMX20_04687 at 40.383 kb on - strand, within MPMX20_04687 at 40.383 kb on - strand, within MPMX20_04687 at 40.383 kb on - strand, within MPMX20_04687 at 40.383 kb on - strand, within MPMX20_04687 at 40.383 kb on - strand, within MPMX20_04687 at 40.393 kb on + strand, within MPMX20_04687 at 40.399 kb on + strand, within MPMX20_04687 at 40.409 kb on + strand, within MPMX20_04687 at 40.473 kb on + strand at 40.494 kb on + strand at 40.495 kb on + strand at 40.502 kb on - strand at 40.503 kb on - strand at 40.505 kb on + strand at 40.505 kb on + strand at 40.505 kb on + strand at 40.505 kb on + strand at 40.505 kb on + strand at 40.505 kb on + strand at 40.505 kb on + strand at 40.532 kb on + strand at 40.542 kb on + strand at 40.542 kb on + strand at 40.550 kb on - strand at 40.550 kb on - strand at 40.557 kb on - strand at 40.567 kb on - strand at 40.567 kb on - strand at 40.602 kb on + strand, within MPMX20_04688 at 40.610 kb on - strand, within MPMX20_04688 at 40.621 kb on + strand, within MPMX20_04688 at 40.624 kb on - strand, within MPMX20_04688 at 40.644 kb on - strand, within MPMX20_04688 at 40.644 kb on - strand, within MPMX20_04688 at 40.644 kb on - strand, within MPMX20_04688 at 40.654 kb on - strand, within MPMX20_04688 at 40.679 kb on + strand, within MPMX20_04688 at 40.679 kb on + strand, within MPMX20_04688 at 40.679 kb on + strand, within MPMX20_04688 at 40.680 kb on - strand, within MPMX20_04688 at 40.687 kb on - strand, within MPMX20_04688 at 40.696 kb on - strand, within MPMX20_04688 at 40.719 kb on + strand, within MPMX20_04688 at 40.719 kb on + strand, within MPMX20_04688 at 40.719 kb on + strand, within MPMX20_04688 at 40.727 kb on - strand, within MPMX20_04688 at 40.735 kb on + strand, within MPMX20_04688 at 40.735 kb on + strand, within MPMX20_04688 at 40.743 kb on - strand, within MPMX20_04688 at 40.743 kb on - strand, within MPMX20_04688 at 40.743 kb on - strand, within MPMX20_04688 at 40.743 kb on - strand, within MPMX20_04688 at 40.758 kb on - strand, within MPMX20_04688 at 40.792 kb on - strand, within MPMX20_04688
Per-strain Table
Position Strand Gene LocusTag Fraction mutant library grown in Supernatant; Paenibacillus favisporus 004-C2 grown in LB remove 38,450 - MPMX20_04684 0.67 -0.0 38,455 + MPMX20_04684 0.67 -2.3 38,478 + MPMX20_04684 0.70 +0.6 38,498 + MPMX20_04684 0.72 -0.5 38,508 - MPMX20_04684 0.73 +0.3 38,527 + MPMX20_04684 0.76 +0.3 38,552 + MPMX20_04684 0.79 -0.4 38,552 + MPMX20_04684 0.79 +1.0 38,552 + MPMX20_04684 0.79 +0.1 38,560 - MPMX20_04684 0.80 -1.9 38,569 + MPMX20_04684 0.81 -0.9 38,605 + MPMX20_04684 0.85 -0.2 38,631 + MPMX20_04684 0.88 +0.4 38,631 + MPMX20_04684 0.88 +1.7 38,631 + MPMX20_04684 0.88 +0.4 38,631 + MPMX20_04684 0.88 +1.3 38,631 + MPMX20_04684 0.88 -0.3 38,631 + MPMX20_04684 0.88 +0.0 38,631 + MPMX20_04684 0.88 +3.0 38,639 - MPMX20_04684 0.89 -0.3 38,639 - MPMX20_04684 0.89 -0.1 38,639 - MPMX20_04684 0.89 +0.1 38,639 - MPMX20_04684 0.89 -0.4 38,639 - MPMX20_04684 0.89 +0.1 38,661 - +0.5 38,661 - -0.4 38,730 + -0.9 38,810 + +0.2 38,810 + +0.6 38,816 - -0.5 38,816 - -0.2 38,830 - +0.3 38,830 - +0.5 38,836 + +1.2 38,844 - -0.5 38,846 - -0.8 38,853 - -0.4 38,853 - +0.2 38,867 - -0.5 38,964 - -0.1 38,967 + +0.1 39,057 + +0.0 39,070 + MPMX20_04685 0.13 +1.0 39,070 + MPMX20_04685 0.13 -0.2 39,070 + MPMX20_04685 0.13 +0.1 39,070 + MPMX20_04685 0.13 +0.6 39,070 + MPMX20_04685 0.13 -0.1 39,070 + MPMX20_04685 0.13 -0.2 39,070 + MPMX20_04685 0.13 -0.0 39,070 + MPMX20_04685 0.13 -0.6 39,070 + MPMX20_04685 0.13 +0.0 39,078 - MPMX20_04685 0.16 -0.1 39,078 - MPMX20_04685 0.16 +0.2 39,078 - MPMX20_04685 0.16 -0.7 39,101 - MPMX20_04685 0.26 -1.0 39,119 + MPMX20_04685 0.33 +0.7 39,124 - MPMX20_04685 0.35 +0.1 39,205 + MPMX20_04685 0.69 -0.2 39,213 - MPMX20_04685 0.72 -0.9 39,213 - MPMX20_04685 0.72 -0.6 39,213 - MPMX20_04685 0.72 -0.7 39,219 + MPMX20_04685 0.74 +0.1 39,219 + MPMX20_04685 0.74 +1.0 39,219 + MPMX20_04685 0.74 +0.5 39,219 + MPMX20_04685 0.74 -0.1 39,227 - MPMX20_04685 0.78 +1.5 39,289 + -0.1 39,289 + -0.3 39,289 + +1.1 39,295 + -0.6 39,320 + +0.0 39,328 - +0.0 39,377 - +0.4 39,377 - +0.1 39,383 - -1.0 39,478 + MPMX20_04686 0.17 -0.6 39,478 + MPMX20_04686 0.17 +0.2 39,478 + MPMX20_04686 0.17 -0.1 39,478 + MPMX20_04686 0.17 -1.4 39,478 + MPMX20_04686 0.17 -1.0 39,478 + MPMX20_04686 0.17 -0.5 39,478 + MPMX20_04686 0.17 -0.8 39,478 + MPMX20_04686 0.17 -0.2 39,478 + MPMX20_04686 0.17 +0.3 39,478 + MPMX20_04686 0.17 -0.4 39,514 + MPMX20_04686 0.27 -0.7 39,546 + MPMX20_04686 0.35 -0.4 39,561 + MPMX20_04686 0.39 -0.6 39,622 - MPMX20_04686 0.55 -6.2 39,622 - MPMX20_04686 0.55 -3.8 39,630 + MPMX20_04686 0.57 -0.6 39,663 - MPMX20_04686 0.66 -2.6 39,687 + MPMX20_04686 0.72 -0.3 39,692 + MPMX20_04686 0.73 +0.4 39,695 - MPMX20_04686 0.74 -3.0 39,716 + MPMX20_04686 0.80 -0.0 39,719 - MPMX20_04686 0.81 -2.4 39,719 - MPMX20_04686 0.81 -2.6 39,723 + MPMX20_04686 0.82 -0.9 39,723 + MPMX20_04686 0.82 -0.2 39,724 - MPMX20_04686 0.82 -0.9 39,724 - MPMX20_04686 0.82 -2.0 39,724 - MPMX20_04686 0.82 -1.6 39,724 - MPMX20_04686 0.82 -5.6 39,731 - MPMX20_04686 0.84 -3.9 39,760 + -0.2 39,765 + -0.1 39,765 + -0.3 39,768 - -4.5 39,768 - -3.4 39,781 + -0.1 39,782 + -1.7 39,790 - -3.0 39,796 - -3.0 39,807 + -0.4 39,807 - -3.0 39,832 - -1.8 39,835 + -0.7 39,843 + -0.5 39,843 + -0.0 39,843 + +0.1 39,843 + -1.1 39,845 + +0.1 39,851 - -3.2 39,851 - -2.0 39,851 - -3.2 39,892 - -1.5 39,892 - -1.5 39,905 - -0.7 39,912 + -0.0 39,912 + -0.4 39,912 + +0.1 39,912 + -0.0 39,912 + -0.1 39,912 + -0.6 39,912 + -0.3 39,917 + +0.1 39,920 - -2.3 39,920 - -2.1 39,920 - -2.4 39,921 - -1.0 39,939 - -1.3 39,962 + -0.5 40,005 - -2.6 40,014 - +0.4 40,045 + +0.1 40,050 + +0.6 40,058 - -1.4 40,058 - -1.9 40,058 - -1.2 40,063 + +0.1 40,063 + +0.1 40,072 + -0.6 40,089 + +0.2 40,094 + -0.4 40,102 + -0.6 40,102 - -0.5 40,131 - -0.8 40,131 - -0.3 40,136 - -1.2 40,146 - -1.3 40,152 + -0.9 40,152 + -0.6 40,168 + MPMX20_04687 0.13 -0.3 40,168 + MPMX20_04687 0.13 +0.1 40,197 - MPMX20_04687 0.21 -0.1 40,211 - MPMX20_04687 0.25 -2.8 40,223 - MPMX20_04687 0.29 -2.4 40,241 + MPMX20_04687 0.34 -0.2 40,243 - MPMX20_04687 0.35 -1.3 40,288 - MPMX20_04687 0.48 -1.0 40,307 + MPMX20_04687 0.54 +0.9 40,308 + MPMX20_04687 0.54 -0.3 40,308 + MPMX20_04687 0.54 -0.2 40,308 + MPMX20_04687 0.54 +1.5 40,311 - MPMX20_04687 0.55 -2.1 40,316 - MPMX20_04687 0.56 -1.3 40,316 - MPMX20_04687 0.56 -0.7 40,316 - MPMX20_04687 0.56 -1.7 40,316 - MPMX20_04687 0.56 -3.0 40,316 - MPMX20_04687 0.56 -5.3 40,341 + MPMX20_04687 0.63 -0.3 40,349 + MPMX20_04687 0.66 +0.5 40,349 - MPMX20_04687 0.66 -2.0 40,360 + MPMX20_04687 0.69 -0.0 40,360 + MPMX20_04687 0.69 +0.1 40,368 - MPMX20_04687 0.71 -0.4 40,368 - MPMX20_04687 0.71 -0.5 40,370 + MPMX20_04687 0.72 +0.2 40,370 + MPMX20_04687 0.72 -0.2 40,370 + MPMX20_04687 0.72 +0.2 40,370 + MPMX20_04687 0.72 -0.4 40,370 + MPMX20_04687 0.72 -0.1 40,370 + MPMX20_04687 0.72 +0.2 40,370 + MPMX20_04687 0.72 +0.0 40,370 + MPMX20_04687 0.72 -0.4 40,370 + MPMX20_04687 0.72 -1.0 40,375 + MPMX20_04687 0.73 -0.4 40,375 + MPMX20_04687 0.73 -0.6 40,375 + MPMX20_04687 0.73 +0.2 40,375 + MPMX20_04687 0.73 -0.7 40,378 - MPMX20_04687 0.74 -1.1 40,378 - MPMX20_04687 0.74 -1.4 40,378 - MPMX20_04687 0.74 -1.1 40,378 - MPMX20_04687 0.74 -1.2 40,383 - MPMX20_04687 0.76 -1.7 40,383 - MPMX20_04687 0.76 -1.8 40,383 - MPMX20_04687 0.76 -2.0 40,383 - MPMX20_04687 0.76 -1.6 40,383 - MPMX20_04687 0.76 -3.3 40,393 + MPMX20_04687 0.79 -0.3 40,399 + MPMX20_04687 0.80 -0.4 40,409 + MPMX20_04687 0.83 +0.3 40,473 + -0.2 40,494 + +0.4 40,495 + -0.3 40,502 - -2.1 40,503 - -5.2 40,505 + +0.7 40,505 + -0.2 40,505 + -0.0 40,505 + +0.3 40,505 + +0.4 40,505 + +0.1 40,505 + -1.0 40,532 + +0.6 40,542 + +0.3 40,542 + +0.2 40,550 - -1.6 40,550 - -0.4 40,557 - -0.4 40,567 - -0.1 40,567 - -0.1 40,602 + MPMX20_04688 0.13 -0.1 40,610 - MPMX20_04688 0.14 -0.5 40,621 + MPMX20_04688 0.16 +0.4 40,624 - MPMX20_04688 0.16 -1.1 40,644 - MPMX20_04688 0.19 -0.0 40,644 - MPMX20_04688 0.19 +0.1 40,644 - MPMX20_04688 0.19 -0.8 40,654 - MPMX20_04688 0.20 -0.8 40,679 + MPMX20_04688 0.23 +0.8 40,679 + MPMX20_04688 0.23 +0.6 40,679 + MPMX20_04688 0.23 -0.1 40,680 - MPMX20_04688 0.24 -0.1 40,687 - MPMX20_04688 0.25 -0.2 40,696 - MPMX20_04688 0.26 -1.2 40,719 + MPMX20_04688 0.29 +0.4 40,719 + MPMX20_04688 0.29 -0.9 40,719 + MPMX20_04688 0.29 +0.5 40,727 - MPMX20_04688 0.30 +0.6 40,735 + MPMX20_04688 0.31 +0.1 40,735 + MPMX20_04688 0.31 +0.1 40,743 - MPMX20_04688 0.32 -0.1 40,743 - MPMX20_04688 0.32 +0.0 40,743 - MPMX20_04688 0.32 +0.1 40,743 - MPMX20_04688 0.32 +0.1 40,758 - MPMX20_04688 0.34 -0.1 40,792 - MPMX20_04688 0.39 -0.4
Or see this region's nucleotide sequence