Experiment: Ying_AminoAcid20 rep A; time point 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE51_RS08015 and ABIE51_RS08020 overlap by 4 nucleotides ABIE51_RS08020 and ABIE51_RS08025 are separated by 28 nucleotides ABIE51_RS08025 and ABIE51_RS08030 are separated by 27 nucleotides
ABIE51_RS08015: ABIE51_RS08015 - polynucleotide adenylyltransferase PcnB, at 1,705,199 to 1,706,524
_RS08015
ABIE51_RS08020: ABIE51_RS08020 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine diphosphokinase, at 1,706,521 to 1,707,051
_RS08020
ABIE51_RS08025: ABIE51_RS08025 - 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 1,707,080 to 1,707,916
_RS08025
ABIE51_RS08030: ABIE51_RS08030 - pantoate--beta-alanine ligase, at 1,707,944 to 1,708,792
_RS08030
Position (kb)
1707
1708 Strain fitness (log2 ratio)
-1
0
1 at 1707.020 kb on + strand at 1707.025 kb on + strand at 1707.025 kb on + strand at 1707.025 kb on + strand at 1707.025 kb on + strand at 1707.025 kb on + strand at 1707.026 kb on - strand at 1707.026 kb on - strand at 1707.026 kb on - strand at 1707.068 kb on + strand at 1707.069 kb on - strand at 1707.083 kb on + strand at 1707.083 kb on + strand at 1707.083 kb on + strand at 1707.908 kb on + strand at 1708.880 kb on + strand at 1708.880 kb on + strand at 1708.880 kb on + strand at 1708.880 kb on + strand at 1708.880 kb on + strand at 1708.881 kb on - strand at 1708.881 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep A; time point 1 remove 1,707,020 + -0.1 1,707,025 + +0.2 1,707,025 + -0.2 1,707,025 + -0.3 1,707,025 + -0.3 1,707,025 + -0.0 1,707,026 - +0.4 1,707,026 - +1.0 1,707,026 - -1.0 1,707,068 + +0.9 1,707,069 - -0.1 1,707,083 + -0.1 1,707,083 + -1.0 1,707,083 + -0.2 1,707,908 + +0.8 1,708,880 + -0.1 1,708,880 + -0.5 1,708,880 + -0.4 1,708,880 + -0.0 1,708,880 + +0.5 1,708,881 - -0.2 1,708,881 - +0.2
Or see this region's nucleotide sequence