Experiment: diet=StandardDiet; sample=FecalPellet; inoculation=Mono; mouse18; day5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1078_RS01120 and HMPREF1078_RS01125 are separated by 102 nucleotides HMPREF1078_RS01125 and HMPREF1078_RS01130 are separated by 87 nucleotides
HMPREF1078_RS01120: HMPREF1078_RS01120 - prephenate dehydrogenase/arogenate dehydrogenase family protein, at 281,410 to 282,183
_RS01120
HMPREF1078_RS01125: HMPREF1078_RS01125 - bifunctional 3-deoxy-7-phosphoheptulonate synthase/chorismate mutase type II, at 282,286 to 283,356
_RS01125
HMPREF1078_RS01130: HMPREF1078_RS01130 - aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme, at 283,444 to 284,631
_RS01130
Position (kb) 282
283
284 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 281.295 kb on + strand at 281.295 kb on + strand at 281.300 kb on + strand at 281.301 kb on - strand at 281.311 kb on + strand at 281.316 kb on - strand at 281.381 kb on + strand at 281.381 kb on + strand at 281.381 kb on + strand at 281.382 kb on - strand at 281.398 kb on + strand at 281.398 kb on + strand at 281.408 kb on + strand at 281.441 kb on + strand at 281.561 kb on + strand, within HMPREF1078_RS01120 at 281.562 kb on - strand, within HMPREF1078_RS01120 at 281.562 kb on - strand, within HMPREF1078_RS01120 at 281.562 kb on - strand, within HMPREF1078_RS01120 at 281.590 kb on + strand, within HMPREF1078_RS01120 at 281.590 kb on + strand, within HMPREF1078_RS01120 at 281.595 kb on - strand, within HMPREF1078_RS01120 at 281.653 kb on + strand, within HMPREF1078_RS01120 at 281.653 kb on + strand, within HMPREF1078_RS01120 at 281.654 kb on - strand, within HMPREF1078_RS01120 at 281.654 kb on - strand, within HMPREF1078_RS01120 at 281.719 kb on - strand, within HMPREF1078_RS01120 at 281.721 kb on - strand, within HMPREF1078_RS01120 at 281.730 kb on + strand, within HMPREF1078_RS01120 at 281.730 kb on + strand, within HMPREF1078_RS01120 at 281.730 kb on + strand, within HMPREF1078_RS01120 at 281.731 kb on - strand, within HMPREF1078_RS01120 at 281.734 kb on + strand, within HMPREF1078_RS01120 at 281.735 kb on - strand, within HMPREF1078_RS01120 at 281.750 kb on + strand, within HMPREF1078_RS01120 at 281.750 kb on + strand, within HMPREF1078_RS01120 at 281.751 kb on - strand, within HMPREF1078_RS01120 at 281.755 kb on - strand, within HMPREF1078_RS01120 at 281.767 kb on + strand, within HMPREF1078_RS01120 at 281.797 kb on - strand, within HMPREF1078_RS01120 at 281.797 kb on - strand, within HMPREF1078_RS01120 at 281.805 kb on + strand, within HMPREF1078_RS01120 at 281.805 kb on + strand, within HMPREF1078_RS01120 at 281.863 kb on - strand, within HMPREF1078_RS01120 at 281.871 kb on - strand, within HMPREF1078_RS01120 at 281.871 kb on - strand, within HMPREF1078_RS01120 at 281.937 kb on + strand, within HMPREF1078_RS01120 at 281.938 kb on - strand, within HMPREF1078_RS01120 at 281.938 kb on - strand, within HMPREF1078_RS01120 at 281.942 kb on + strand, within HMPREF1078_RS01120 at 281.943 kb on - strand, within HMPREF1078_RS01120 at 281.943 kb on - strand, within HMPREF1078_RS01120 at 281.954 kb on + strand, within HMPREF1078_RS01120 at 281.985 kb on + strand, within HMPREF1078_RS01120 at 281.985 kb on + strand, within HMPREF1078_RS01120 at 281.988 kb on - strand, within HMPREF1078_RS01120 at 281.992 kb on + strand, within HMPREF1078_RS01120 at 281.993 kb on - strand, within HMPREF1078_RS01120 at 281.993 kb on - strand, within HMPREF1078_RS01120 at 281.993 kb on - strand, within HMPREF1078_RS01120 at 282.069 kb on - strand, within HMPREF1078_RS01120 at 282.069 kb on - strand, within HMPREF1078_RS01120 at 282.069 kb on - strand, within HMPREF1078_RS01120 at 282.069 kb on - strand, within HMPREF1078_RS01120 at 282.069 kb on - strand, within HMPREF1078_RS01120 at 282.103 kb on + strand, within HMPREF1078_RS01120 at 282.109 kb on + strand at 282.109 kb on + strand at 282.126 kb on + strand at 282.130 kb on + strand at 282.131 kb on - strand at 282.169 kb on + strand at 282.176 kb on - strand at 282.190 kb on + strand at 282.190 kb on + strand at 282.191 kb on - strand at 282.204 kb on - strand at 282.204 kb on - strand at 282.206 kb on + strand at 282.207 kb on - strand at 282.207 kb on - strand at 282.207 kb on - strand at 282.218 kb on - strand at 282.220 kb on + strand at 282.220 kb on - strand at 282.221 kb on - strand at 282.240 kb on + strand at 282.262 kb on + strand at 282.263 kb on - strand at 282.265 kb on + strand at 282.266 kb on - strand at 282.266 kb on - strand at 282.281 kb on + strand at 282.282 kb on - strand at 282.284 kb on + strand at 282.284 kb on + strand at 282.284 kb on + strand at 282.285 kb on - strand at 282.285 kb on - strand at 282.287 kb on + strand at 282.288 kb on - strand at 282.392 kb on + strand at 282.444 kb on + strand, within HMPREF1078_RS01125 at 282.444 kb on + strand, within HMPREF1078_RS01125 at 282.445 kb on - strand, within HMPREF1078_RS01125 at 282.445 kb on - strand, within HMPREF1078_RS01125 at 282.445 kb on - strand, within HMPREF1078_RS01125 at 282.445 kb on - strand, within HMPREF1078_RS01125 at 282.445 kb on - strand, within HMPREF1078_RS01125 at 282.484 kb on - strand, within HMPREF1078_RS01125 at 282.484 kb on - strand, within HMPREF1078_RS01125 at 282.484 kb on - strand, within HMPREF1078_RS01125 at 282.489 kb on + strand, within HMPREF1078_RS01125 at 282.489 kb on + strand, within HMPREF1078_RS01125 at 282.490 kb on - strand, within HMPREF1078_RS01125 at 282.606 kb on - strand, within HMPREF1078_RS01125 at 282.606 kb on - strand, within HMPREF1078_RS01125 at 282.606 kb on - strand, within HMPREF1078_RS01125 at 282.606 kb on - strand, within HMPREF1078_RS01125 at 282.606 kb on - strand, within HMPREF1078_RS01125 at 282.614 kb on - strand, within HMPREF1078_RS01125 at 282.811 kb on - strand, within HMPREF1078_RS01125 at 282.834 kb on - strand, within HMPREF1078_RS01125 at 282.834 kb on - strand, within HMPREF1078_RS01125 at 282.843 kb on + strand, within HMPREF1078_RS01125 at 282.843 kb on + strand, within HMPREF1078_RS01125 at 282.844 kb on - strand, within HMPREF1078_RS01125 at 282.844 kb on - strand, within HMPREF1078_RS01125 at 282.849 kb on - strand, within HMPREF1078_RS01125 at 282.849 kb on - strand, within HMPREF1078_RS01125 at 282.849 kb on - strand, within HMPREF1078_RS01125 at 282.899 kb on + strand, within HMPREF1078_RS01125 at 282.900 kb on - strand, within HMPREF1078_RS01125 at 282.900 kb on - strand, within HMPREF1078_RS01125 at 282.973 kb on + strand, within HMPREF1078_RS01125 at 282.973 kb on + strand, within HMPREF1078_RS01125 at 282.974 kb on - strand, within HMPREF1078_RS01125 at 282.974 kb on - strand, within HMPREF1078_RS01125 at 283.027 kb on - strand, within HMPREF1078_RS01125 at 283.109 kb on + strand, within HMPREF1078_RS01125 at 283.109 kb on + strand, within HMPREF1078_RS01125 at 283.109 kb on + strand, within HMPREF1078_RS01125 at 283.110 kb on - strand, within HMPREF1078_RS01125 at 283.110 kb on - strand, within HMPREF1078_RS01125 at 283.110 kb on - strand, within HMPREF1078_RS01125 at 283.110 kb on - strand, within HMPREF1078_RS01125 at 283.110 kb on - strand, within HMPREF1078_RS01125 at 283.110 kb on - strand, within HMPREF1078_RS01125 at 283.110 kb on - strand, within HMPREF1078_RS01125 at 283.110 kb on - strand, within HMPREF1078_RS01125 at 283.110 kb on - strand, within HMPREF1078_RS01125 at 283.113 kb on + strand, within HMPREF1078_RS01125 at 283.113 kb on + strand, within HMPREF1078_RS01125 at 283.113 kb on + strand, within HMPREF1078_RS01125 at 283.114 kb on - strand, within HMPREF1078_RS01125 at 283.114 kb on - strand, within HMPREF1078_RS01125 at 283.258 kb on + strand at 283.259 kb on - strand at 283.281 kb on + strand at 283.298 kb on - strand at 283.303 kb on - strand at 283.318 kb on + strand at 283.319 kb on - strand at 283.362 kb on + strand at 283.363 kb on - strand at 283.374 kb on + strand at 283.375 kb on - strand at 283.380 kb on - strand at 283.387 kb on - strand at 283.387 kb on - strand at 283.389 kb on - strand at 283.393 kb on + strand at 283.401 kb on - strand at 283.413 kb on + strand at 283.429 kb on + strand at 283.430 kb on - strand at 283.430 kb on - strand at 283.433 kb on + strand at 283.433 kb on + strand at 283.434 kb on - strand at 283.434 kb on - strand at 283.434 kb on - strand at 283.441 kb on - strand at 283.454 kb on + strand at 283.454 kb on + strand at 283.466 kb on - strand at 283.501 kb on - strand at 283.515 kb on + strand at 283.516 kb on - strand at 283.516 kb on - strand at 283.524 kb on - strand at 283.561 kb on + strand at 283.647 kb on + strand, within HMPREF1078_RS01130 at 283.647 kb on + strand, within HMPREF1078_RS01130 at 283.647 kb on + strand, within HMPREF1078_RS01130 at 283.648 kb on - strand, within HMPREF1078_RS01130 at 283.651 kb on + strand, within HMPREF1078_RS01130 at 283.651 kb on + strand, within HMPREF1078_RS01130 at 283.652 kb on - strand, within HMPREF1078_RS01130 at 283.721 kb on + strand, within HMPREF1078_RS01130 at 283.724 kb on - strand, within HMPREF1078_RS01130 at 283.734 kb on + strand, within HMPREF1078_RS01130 at 283.734 kb on + strand, within HMPREF1078_RS01130 at 283.735 kb on - strand, within HMPREF1078_RS01130 at 283.743 kb on - strand, within HMPREF1078_RS01130 at 283.743 kb on - strand, within HMPREF1078_RS01130 at 283.822 kb on + strand, within HMPREF1078_RS01130 at 283.841 kb on - strand, within HMPREF1078_RS01130 at 283.872 kb on + strand, within HMPREF1078_RS01130 at 283.887 kb on + strand, within HMPREF1078_RS01130 at 283.888 kb on - strand, within HMPREF1078_RS01130 at 283.900 kb on - strand, within HMPREF1078_RS01130 at 283.918 kb on + strand, within HMPREF1078_RS01130 at 283.918 kb on + strand, within HMPREF1078_RS01130 at 283.920 kb on + strand, within HMPREF1078_RS01130 at 283.983 kb on + strand, within HMPREF1078_RS01130 at 283.984 kb on - strand, within HMPREF1078_RS01130 at 283.984 kb on - strand, within HMPREF1078_RS01130 at 284.064 kb on + strand, within HMPREF1078_RS01130 at 284.080 kb on - strand, within HMPREF1078_RS01130 at 284.159 kb on - strand, within HMPREF1078_RS01130 at 284.217 kb on + strand, within HMPREF1078_RS01130 at 284.217 kb on + strand, within HMPREF1078_RS01130 at 284.217 kb on + strand, within HMPREF1078_RS01130 at 284.218 kb on - strand, within HMPREF1078_RS01130 at 284.219 kb on + strand, within HMPREF1078_RS01130 at 284.281 kb on + strand, within HMPREF1078_RS01130 at 284.281 kb on + strand, within HMPREF1078_RS01130 at 284.282 kb on - strand, within HMPREF1078_RS01130 at 284.351 kb on + strand, within HMPREF1078_RS01130
Per-strain Table
Position Strand Gene LocusTag Fraction diet=StandardDiet; sample=FecalPellet; inoculation=Mono; mouse18; day5 remove 281,295 + +0.4 281,295 + -1.9 281,300 + -2.4 281,301 - -0.6 281,311 + -2.2 281,316 - -0.1 281,381 + +1.5 281,381 + -0.5 281,381 + +0.3 281,382 - +0.3 281,398 + +2.0 281,398 + -1.2 281,408 + +0.1 281,441 + -1.4 281,561 + HMPREF1078_RS01120 0.20 -0.9 281,562 - HMPREF1078_RS01120 0.20 -1.2 281,562 - HMPREF1078_RS01120 0.20 -1.4 281,562 - HMPREF1078_RS01120 0.20 -1.0 281,590 + HMPREF1078_RS01120 0.23 -2.2 281,590 + HMPREF1078_RS01120 0.23 -1.1 281,595 - HMPREF1078_RS01120 0.24 -0.6 281,653 + HMPREF1078_RS01120 0.31 +0.4 281,653 + HMPREF1078_RS01120 0.31 -1.0 281,654 - HMPREF1078_RS01120 0.32 -3.5 281,654 - HMPREF1078_RS01120 0.32 -3.4 281,719 - HMPREF1078_RS01120 0.40 -3.7 281,721 - HMPREF1078_RS01120 0.40 -2.0 281,730 + HMPREF1078_RS01120 0.41 +1.4 281,730 + HMPREF1078_RS01120 0.41 -1.9 281,730 + HMPREF1078_RS01120 0.41 -1.2 281,731 - HMPREF1078_RS01120 0.41 -0.7 281,734 + HMPREF1078_RS01120 0.42 +0.1 281,735 - HMPREF1078_RS01120 0.42 -0.7 281,750 + HMPREF1078_RS01120 0.44 +1.6 281,750 + HMPREF1078_RS01120 0.44 -2.9 281,751 - HMPREF1078_RS01120 0.44 +0.9 281,755 - HMPREF1078_RS01120 0.45 -3.5 281,767 + HMPREF1078_RS01120 0.46 -3.1 281,797 - HMPREF1078_RS01120 0.50 -1.6 281,797 - HMPREF1078_RS01120 0.50 -2.5 281,805 + HMPREF1078_RS01120 0.51 -1.2 281,805 + HMPREF1078_RS01120 0.51 -1.0 281,863 - HMPREF1078_RS01120 0.59 -2.0 281,871 - HMPREF1078_RS01120 0.60 -3.8 281,871 - HMPREF1078_RS01120 0.60 -1.0 281,937 + HMPREF1078_RS01120 0.68 -1.2 281,938 - HMPREF1078_RS01120 0.68 -0.9 281,938 - HMPREF1078_RS01120 0.68 -2.8 281,942 + HMPREF1078_RS01120 0.69 -2.5 281,943 - HMPREF1078_RS01120 0.69 -0.9 281,943 - HMPREF1078_RS01120 0.69 -1.2 281,954 + HMPREF1078_RS01120 0.70 +0.6 281,985 + HMPREF1078_RS01120 0.74 -2.5 281,985 + HMPREF1078_RS01120 0.74 -1.1 281,988 - HMPREF1078_RS01120 0.75 -2.2 281,992 + HMPREF1078_RS01120 0.75 -2.1 281,993 - HMPREF1078_RS01120 0.75 -2.3 281,993 - HMPREF1078_RS01120 0.75 -1.6 281,993 - HMPREF1078_RS01120 0.75 -2.8 282,069 - HMPREF1078_RS01120 0.85 -1.4 282,069 - HMPREF1078_RS01120 0.85 -2.5 282,069 - HMPREF1078_RS01120 0.85 -3.2 282,069 - HMPREF1078_RS01120 0.85 +0.6 282,069 - HMPREF1078_RS01120 0.85 -1.6 282,103 + HMPREF1078_RS01120 0.90 -1.6 282,109 + -2.9 282,109 + -2.4 282,126 + -2.6 282,130 + -3.5 282,131 - -1.0 282,169 + +0.0 282,176 - -1.7 282,190 + -1.0 282,190 + -2.2 282,191 - +1.0 282,204 - -1.1 282,204 - -0.4 282,206 + -0.6 282,207 - +1.4 282,207 - -0.5 282,207 - -1.2 282,218 - -1.2 282,220 + -1.0 282,220 - -2.7 282,221 - -1.2 282,240 + -2.2 282,262 + -0.2 282,263 - -4.1 282,265 + +0.0 282,266 - +0.6 282,266 - -3.1 282,281 + -1.6 282,282 - -1.0 282,284 + -2.2 282,284 + -2.7 282,284 + -0.4 282,285 - +0.1 282,285 - -1.0 282,287 + +0.9 282,288 - +0.8 282,392 + -2.8 282,444 + HMPREF1078_RS01125 0.15 -0.3 282,444 + HMPREF1078_RS01125 0.15 -2.0 282,445 - HMPREF1078_RS01125 0.15 -2.1 282,445 - HMPREF1078_RS01125 0.15 -1.6 282,445 - HMPREF1078_RS01125 0.15 -1.4 282,445 - HMPREF1078_RS01125 0.15 -1.0 282,445 - HMPREF1078_RS01125 0.15 -2.3 282,484 - HMPREF1078_RS01125 0.18 -1.3 282,484 - HMPREF1078_RS01125 0.18 -1.2 282,484 - HMPREF1078_RS01125 0.18 -2.7 282,489 + HMPREF1078_RS01125 0.19 -0.0 282,489 + HMPREF1078_RS01125 0.19 +0.6 282,490 - HMPREF1078_RS01125 0.19 -2.1 282,606 - HMPREF1078_RS01125 0.30 +1.0 282,606 - HMPREF1078_RS01125 0.30 +0.4 282,606 - HMPREF1078_RS01125 0.30 -1.3 282,606 - HMPREF1078_RS01125 0.30 -2.7 282,606 - HMPREF1078_RS01125 0.30 -0.0 282,614 - HMPREF1078_RS01125 0.31 +0.6 282,811 - HMPREF1078_RS01125 0.49 -0.7 282,834 - HMPREF1078_RS01125 0.51 -2.7 282,834 - HMPREF1078_RS01125 0.51 +1.6 282,843 + HMPREF1078_RS01125 0.52 -1.6 282,843 + HMPREF1078_RS01125 0.52 -2.0 282,844 - HMPREF1078_RS01125 0.52 -0.0 282,844 - HMPREF1078_RS01125 0.52 -2.1 282,849 - HMPREF1078_RS01125 0.53 -1.8 282,849 - HMPREF1078_RS01125 0.53 -3.1 282,849 - HMPREF1078_RS01125 0.53 -1.4 282,899 + HMPREF1078_RS01125 0.57 -1.0 282,900 - HMPREF1078_RS01125 0.57 -2.7 282,900 - HMPREF1078_RS01125 0.57 -1.2 282,973 + HMPREF1078_RS01125 0.64 -2.4 282,973 + HMPREF1078_RS01125 0.64 -0.7 282,974 - HMPREF1078_RS01125 0.64 -2.5 282,974 - HMPREF1078_RS01125 0.64 -2.8 283,027 - HMPREF1078_RS01125 0.69 -0.0 283,109 + HMPREF1078_RS01125 0.77 +1.6 283,109 + HMPREF1078_RS01125 0.77 +0.6 283,109 + HMPREF1078_RS01125 0.77 -1.6 283,110 - HMPREF1078_RS01125 0.77 -1.7 283,110 - HMPREF1078_RS01125 0.77 -3.1 283,110 - HMPREF1078_RS01125 0.77 -1.0 283,110 - HMPREF1078_RS01125 0.77 -1.0 283,110 - HMPREF1078_RS01125 0.77 -1.3 283,110 - HMPREF1078_RS01125 0.77 -1.0 283,110 - HMPREF1078_RS01125 0.77 -1.6 283,110 - HMPREF1078_RS01125 0.77 -1.9 283,110 - HMPREF1078_RS01125 0.77 -2.1 283,113 + HMPREF1078_RS01125 0.77 +1.1 283,113 + HMPREF1078_RS01125 0.77 -1.0 283,113 + HMPREF1078_RS01125 0.77 -1.1 283,114 - HMPREF1078_RS01125 0.77 +0.3 283,114 - HMPREF1078_RS01125 0.77 -1.2 283,258 + -1.0 283,259 - -1.4 283,281 + -2.1 283,298 - -0.7 283,303 - -1.7 283,318 + -2.5 283,319 - -0.4 283,362 + -0.4 283,363 - +0.7 283,374 + -1.0 283,375 - +0.7 283,380 - +0.4 283,387 - -0.5 283,387 - +1.0 283,389 - -0.7 283,393 + -1.9 283,401 - -1.4 283,413 + -3.1 283,429 + -1.9 283,430 - -0.0 283,430 - +1.2 283,433 + -0.4 283,433 + -0.5 283,434 - -0.7 283,434 - -0.0 283,434 - +0.7 283,441 - +0.6 283,454 + -2.4 283,454 + -3.5 283,466 - -1.2 283,501 - -0.2 283,515 + -0.7 283,516 - +1.6 283,516 - -2.3 283,524 - +0.2 283,561 + -1.6 283,647 + HMPREF1078_RS01130 0.17 -1.9 283,647 + HMPREF1078_RS01130 0.17 +2.6 283,647 + HMPREF1078_RS01130 0.17 -0.5 283,648 - HMPREF1078_RS01130 0.17 -1.0 283,651 + HMPREF1078_RS01130 0.17 -1.7 283,651 + HMPREF1078_RS01130 0.17 -1.3 283,652 - HMPREF1078_RS01130 0.18 +0.6 283,721 + HMPREF1078_RS01130 0.23 -1.5 283,724 - HMPREF1078_RS01130 0.24 +0.3 283,734 + HMPREF1078_RS01130 0.24 -0.4 283,734 + HMPREF1078_RS01130 0.24 -1.0 283,735 - HMPREF1078_RS01130 0.24 +0.6 283,743 - HMPREF1078_RS01130 0.25 +0.6 283,743 - HMPREF1078_RS01130 0.25 +0.3 283,822 + HMPREF1078_RS01130 0.32 -1.6 283,841 - HMPREF1078_RS01130 0.33 -0.2 283,872 + HMPREF1078_RS01130 0.36 -2.9 283,887 + HMPREF1078_RS01130 0.37 +1.6 283,888 - HMPREF1078_RS01130 0.37 -1.6 283,900 - HMPREF1078_RS01130 0.38 -0.0 283,918 + HMPREF1078_RS01130 0.40 -3.2 283,918 + HMPREF1078_RS01130 0.40 -0.4 283,920 + HMPREF1078_RS01130 0.40 -2.1 283,983 + HMPREF1078_RS01130 0.45 -1.4 283,984 - HMPREF1078_RS01130 0.45 -0.4 283,984 - HMPREF1078_RS01130 0.45 -1.4 284,064 + HMPREF1078_RS01130 0.52 -0.7 284,080 - HMPREF1078_RS01130 0.54 -2.7 284,159 - HMPREF1078_RS01130 0.60 -0.7 284,217 + HMPREF1078_RS01130 0.65 -0.4 284,217 + HMPREF1078_RS01130 0.65 -0.8 284,217 + HMPREF1078_RS01130 0.65 -1.4 284,218 - HMPREF1078_RS01130 0.65 -0.0 284,219 + HMPREF1078_RS01130 0.65 -0.0 284,281 + HMPREF1078_RS01130 0.70 -1.3 284,281 + HMPREF1078_RS01130 0.70 +1.6 284,282 - HMPREF1078_RS01130 0.71 -0.3 284,351 + HMPREF1078_RS01130 0.76 -2.0
Or see this region's nucleotide sequence