Experiment: 7H9 with Streptomycin 0.178 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv1552 and Rv1553 are separated by 2 nucleotides Rv1553 and Rv1554 overlap by 4 nucleotides Rv1554 and Rv1555 overlap by 4 nucleotides Rv1555 and Rv1556 are separated by 67 nucleotides
Rv1552: Rv1552 - Probable fumarate reductase [flavoprotein subunit] FrdA (fumarate dehydrogenase) (fumaric hydrogenase), at 1,757,681 to 1,759,432
Rv1552
Rv1553: Rv1553 - Probable fumarate reductase [iron-sulfur subunit] FrdB (fumarate dehydrogenase) (fumaric hydrogenase), at 1,759,435 to 1,760,178
Rv1553
Rv1554: Rv1554 - Probable fumarate reductase [membrane anchor subunit] FrdC (fumarate dehydrogenase) (fumaric hydrogenase), at 1,760,175 to 1,760,555
Rv1554
Rv1555: Rv1555 - Probable fumarate reductase [membrane anchor subunit] FrdD (fumarate dehydrogenase) (fumaric hydrogenase), at 1,760,552 to 1,760,929
Rv1555
Rv1556: Rv1556 - Possible regulatory protein, at 1,760,997 to 1,761,605
Rv1556
Position (kb)
1759
1760
1761 Strain fitness (log2 ratio)
-1
0
1
2 at 1758.465 kb on - strand, within Rv1552 at 1758.559 kb on - strand, within Rv1552 at 1758.596 kb on + strand, within Rv1552 at 1758.615 kb on + strand, within Rv1552 at 1758.666 kb on + strand, within Rv1552 at 1758.667 kb on - strand, within Rv1552 at 1758.795 kb on + strand, within Rv1552 at 1758.829 kb on + strand, within Rv1552 at 1758.985 kb on - strand, within Rv1552 at 1759.081 kb on + strand, within Rv1552 at 1759.086 kb on - strand, within Rv1552 at 1759.422 kb on - strand at 1759.422 kb on - strand at 1759.430 kb on + strand at 1759.430 kb on + strand at 1759.430 kb on + strand at 1759.431 kb on - strand at 1759.431 kb on - strand at 1759.431 kb on - strand at 1759.555 kb on + strand, within Rv1553 at 1759.555 kb on + strand, within Rv1553 at 1759.555 kb on + strand, within Rv1553 at 1759.555 kb on + strand, within Rv1553 at 1759.611 kb on + strand, within Rv1553 at 1759.629 kb on + strand, within Rv1553 at 1759.630 kb on - strand, within Rv1553 at 1759.681 kb on + strand, within Rv1553 at 1759.682 kb on - strand, within Rv1553 at 1759.686 kb on - strand, within Rv1553 at 1759.686 kb on - strand, within Rv1553 at 1759.882 kb on - strand, within Rv1553 at 1759.882 kb on - strand, within Rv1553 at 1759.973 kb on - strand, within Rv1553 at 1760.116 kb on + strand at 1760.229 kb on + strand, within Rv1554 at 1760.229 kb on + strand, within Rv1554 at 1760.229 kb on + strand, within Rv1554 at 1760.287 kb on - strand, within Rv1554 at 1760.300 kb on - strand, within Rv1554 at 1760.371 kb on + strand, within Rv1554 at 1760.372 kb on - strand, within Rv1554 at 1760.418 kb on - strand, within Rv1554 at 1760.923 kb on + strand at 1760.923 kb on + strand at 1760.924 kb on - strand at 1760.927 kb on + strand at 1760.927 kb on + strand at 1760.928 kb on - strand at 1760.996 kb on + strand at 1760.997 kb on - strand at 1761.001 kb on + strand at 1761.085 kb on + strand, within Rv1556 at 1761.085 kb on + strand, within Rv1556 at 1761.086 kb on - strand, within Rv1556 at 1761.118 kb on + strand, within Rv1556
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Streptomycin 0.178 ug/mL remove 1,758,465 - Rv1552 0.45 -0.1 1,758,559 - Rv1552 0.50 -0.8 1,758,596 + Rv1552 0.52 +0.1 1,758,615 + Rv1552 0.53 +0.9 1,758,666 + Rv1552 0.56 -0.2 1,758,667 - Rv1552 0.56 -0.4 1,758,795 + Rv1552 0.64 -1.6 1,758,829 + Rv1552 0.66 +0.4 1,758,985 - Rv1552 0.74 -0.7 1,759,081 + Rv1552 0.80 -1.7 1,759,086 - Rv1552 0.80 -0.8 1,759,422 - +0.1 1,759,422 - +0.3 1,759,430 + +0.1 1,759,430 + +0.5 1,759,430 + +0.0 1,759,431 - -1.0 1,759,431 - -1.1 1,759,431 - +0.9 1,759,555 + Rv1553 0.16 -0.0 1,759,555 + Rv1553 0.16 -0.1 1,759,555 + Rv1553 0.16 -0.1 1,759,555 + Rv1553 0.16 -0.7 1,759,611 + Rv1553 0.24 -0.3 1,759,629 + Rv1553 0.26 -1.0 1,759,630 - Rv1553 0.26 -1.7 1,759,681 + Rv1553 0.33 -0.9 1,759,682 - Rv1553 0.33 -0.0 1,759,686 - Rv1553 0.34 -0.1 1,759,686 - Rv1553 0.34 -0.2 1,759,882 - Rv1553 0.60 +0.0 1,759,882 - Rv1553 0.60 -0.8 1,759,973 - Rv1553 0.72 -0.7 1,760,116 + -0.3 1,760,229 + Rv1554 0.14 +2.2 1,760,229 + Rv1554 0.14 -0.4 1,760,229 + Rv1554 0.14 -0.2 1,760,287 - Rv1554 0.29 +0.3 1,760,300 - Rv1554 0.33 +0.1 1,760,371 + Rv1554 0.51 -0.5 1,760,372 - Rv1554 0.52 -0.7 1,760,418 - Rv1554 0.64 -0.4 1,760,923 + -0.3 1,760,923 + +0.1 1,760,924 - +0.1 1,760,927 + -0.2 1,760,927 + -0.3 1,760,928 - -0.4 1,760,996 + -0.7 1,760,997 - -0.9 1,761,001 + +0.1 1,761,085 + Rv1556 0.14 -0.4 1,761,085 + Rv1556 0.14 -0.1 1,761,086 - Rv1556 0.15 -0.9 1,761,118 + Rv1556 0.20 -0.2
Or see this region's nucleotide sequence