Experiment: 7H9 with Streptomycin 0.178 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv0552 and Rv0553 overlap by 4 nucleotides Rv0553 and Rv0554 overlap by 4 nucleotides Rv0554 and Rv0555 are separated by 42 nucleotides
Rv0552: Rv0552 - Conserved protein, at 642,889 to 644,493
Rv0552
Rv0553: Rv0553 - Probable muconate cycloisomerase MenC (cis,cis-muconate lactonizing enzyme) (MLE), at 644,490 to 645,470
Rv0553
Rv0554: Rv0554 - Possible peroxidase BpoC (non-haem peroxidase), at 645,467 to 646,255
Rv0554
Rv0555: Rv0555 - Probable bifunctional menaquinone biosynthesis protein MenD : 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (SHCHC synthase) + 2-oxoglutarate decarboxylase (alpha-ketoglutarate decarboxylase) (KDC), at 646,298 to 647,962
Rv0555
Position (kb) 645
646
647 Strain fitness (log2 ratio) -1
0
1
2 at 645.694 kb on - strand, within Rv0554 at 645.694 kb on - strand, within Rv0554 at 645.857 kb on + strand, within Rv0554 at 645.858 kb on - strand, within Rv0554 at 645.887 kb on + strand, within Rv0554 at 645.887 kb on + strand, within Rv0554 at 645.887 kb on + strand, within Rv0554 at 645.906 kb on + strand, within Rv0554 at 645.907 kb on - strand, within Rv0554 at 645.989 kb on - strand, within Rv0554 at 645.989 kb on - strand, within Rv0554 at 646.018 kb on - strand, within Rv0554 at 646.116 kb on - strand, within Rv0554 at 646.293 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Streptomycin 0.178 ug/mL remove 645,694 - Rv0554 0.29 -0.3 645,694 - Rv0554 0.29 +0.7 645,857 + Rv0554 0.49 +0.7 645,858 - Rv0554 0.50 +0.7 645,887 + Rv0554 0.53 +0.7 645,887 + Rv0554 0.53 +1.3 645,887 + Rv0554 0.53 +2.2 645,906 + Rv0554 0.56 +0.8 645,907 - Rv0554 0.56 -0.1 645,989 - Rv0554 0.66 +0.7 645,989 - Rv0554 0.66 +0.6 646,018 - Rv0554 0.70 +0.5 646,116 - Rv0554 0.82 -0.1 646,293 + -1.2
Or see this region's nucleotide sequence