Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv3527

Experiment: 7H9 with Streptomycin 0.178 ug/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv3526 and Rv3527 are separated by 5 nucleotidesRv3527 and Rv3528c are separated by 424 nucleotides Rv3526: Rv3526 - Oxygenase component of 3-ketosteroid-9-alpha-hydroxylase KshA, at 3,962,439 to 3,963,599 Rv3526 Rv3527: Rv3527 - Hypothetical protein, at 3,963,605 to 3,964,054 Rv3527 Rv3528c: Rv3528c - Unknown protein, at 3,964,479 to 3,965,192 Rv3528c Position (kb) 3963 3964 3965Strain fitness (log2 ratio) -1 0 1 2at 3962.748 kb on + strand, within Rv3526at 3962.749 kb on - strand, within Rv3526at 3962.749 kb on - strand, within Rv3526at 3962.749 kb on - strand, within Rv3526at 3962.749 kb on - strand, within Rv3526at 3962.973 kb on - strand, within Rv3526at 3962.973 kb on - strand, within Rv3526at 3962.973 kb on - strand, within Rv3526at 3962.988 kb on + strand, within Rv3526at 3963.012 kb on + strand, within Rv3526at 3963.051 kb on + strand, within Rv3526at 3963.099 kb on + strand, within Rv3526at 3963.099 kb on + strand, within Rv3526at 3963.184 kb on - strand, within Rv3526at 3963.279 kb on - strand, within Rv3526at 3963.418 kb on - strand, within Rv3526at 3963.435 kb on + strand, within Rv3526at 3963.807 kb on - strand, within Rv3527at 3964.026 kb on - strandat 3964.152 kb on - strandat 3964.152 kb on - strandat 3964.152 kb on - strandat 3964.152 kb on - strandat 3964.152 kb on - strandat 3964.276 kb on - strandat 3964.793 kb on + strand, within Rv3528cat 3964.826 kb on - strand, within Rv3528cat 3964.829 kb on + strand, within Rv3528cat 3964.838 kb on + strand, within Rv3528cat 3964.839 kb on - strand, within Rv3528cat 3964.946 kb on + strand, within Rv3528cat 3965.014 kb on - strand, within Rv3528c

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Streptomycin 0.178 ug/mL
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3,962,748 + Rv3526 0.27 +0.1
3,962,749 - Rv3526 0.27 +0.5
3,962,749 - Rv3526 0.27 +0.8
3,962,749 - Rv3526 0.27 +0.1
3,962,749 - Rv3526 0.27 +0.0
3,962,973 - Rv3526 0.46 -0.5
3,962,973 - Rv3526 0.46 +0.0
3,962,973 - Rv3526 0.46 -0.2
3,962,988 + Rv3526 0.47 -0.7
3,963,012 + Rv3526 0.49 +0.6
3,963,051 + Rv3526 0.53 +0.7
3,963,099 + Rv3526 0.57 -0.5
3,963,099 + Rv3526 0.57 +0.7
3,963,184 - Rv3526 0.64 -0.5
3,963,279 - Rv3526 0.72 -1.0
3,963,418 - Rv3526 0.84 +1.0
3,963,435 + Rv3526 0.86 +0.2
3,963,807 - Rv3527 0.45 -1.2
3,964,026 - -0.4
3,964,152 - +1.4
3,964,152 - +0.2
3,964,152 - +0.2
3,964,152 - +2.0
3,964,152 - -0.9
3,964,276 - -0.2
3,964,793 + Rv3528c 0.44 -0.5
3,964,826 - Rv3528c 0.49 +0.2
3,964,829 + Rv3528c 0.49 +0.1
3,964,838 + Rv3528c 0.50 -0.3
3,964,839 - Rv3528c 0.50 +1.6
3,964,946 + Rv3528c 0.65 +0.2
3,965,014 - Rv3528c 0.75 -0.7

Or see this region's nucleotide sequence