Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv3515c

Experiment: 7H9 with Streptomycin 0.178 ug/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv3514 and Rv3515c are separated by 560 nucleotidesRv3515c and Rv3516 are separated by 73 nucleotidesRv3516 and Rv3517 are separated by 95 nucleotides Rv3514: Rv3514 - PE-PGRS family protein PE_PGRS57, at 3,945,794 to 3,950,263 Rv3514 Rv3515c: Rv3515c - Fatty-acid-CoA ligase FadD19 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase), at 3,950,824 to 3,952,470 Rv3515c Rv3516: Rv3516 - Possible enoyl-CoA hydratase EchA19 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase), at 3,952,544 to 3,953,335 Rv3516 Rv3517: Rv3517 - Conserved hypothetical protein, at 3,953,431 to 3,954,270 Rv3517 Position (kb) 3950 3951 3952 3953Strain fitness (log2 ratio) -1 0 1 2at 3950.108 kb on - strandat 3950.117 kb on - strandat 3950.125 kb on + strandat 3950.351 kb on + strandat 3950.402 kb on + strandat 3950.402 kb on + strandat 3950.402 kb on + strandat 3950.402 kb on + strandat 3950.403 kb on - strandat 3950.410 kb on - strandat 3950.611 kb on - strandat 3950.622 kb on - strandat 3950.690 kb on - strandat 3950.690 kb on - strandat 3950.720 kb on + strandat 3950.720 kb on + strandat 3950.720 kb on + strandat 3950.720 kb on + strandat 3951.839 kb on + strand, within Rv3515cat 3951.840 kb on - strand, within Rv3515cat 3951.953 kb on + strand, within Rv3515cat 3951.953 kb on + strand, within Rv3515cat 3951.962 kb on + strand, within Rv3515cat 3951.962 kb on + strand, within Rv3515cat 3951.962 kb on + strand, within Rv3515cat 3951.963 kb on - strand, within Rv3515cat 3951.963 kb on - strand, within Rv3515cat 3952.093 kb on + strand, within Rv3515cat 3952.093 kb on + strand, within Rv3515cat 3952.093 kb on + strand, within Rv3515cat 3952.094 kb on - strand, within Rv3515cat 3952.094 kb on - strand, within Rv3515cat 3952.185 kb on - strand, within Rv3515cat 3952.290 kb on - strand, within Rv3515cat 3952.290 kb on - strand, within Rv3515cat 3952.356 kb on + strandat 3952.374 kb on - strandat 3952.453 kb on - strandat 3952.453 kb on - strandat 3952.797 kb on - strand, within Rv3516at 3953.130 kb on + strand, within Rv3516

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Streptomycin 0.178 ug/mL
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3,950,108 - +0.3
3,950,117 - +0.6
3,950,125 + +0.4
3,950,351 + +0.1
3,950,402 + +0.4
3,950,402 + -0.5
3,950,402 + -0.3
3,950,402 + +0.2
3,950,403 - +1.5
3,950,410 - +1.3
3,950,611 - +0.3
3,950,622 - +1.6
3,950,690 - +1.1
3,950,690 - +0.2
3,950,720 + +2.0
3,950,720 + -1.6
3,950,720 + +0.4
3,950,720 + +0.1
3,951,839 + Rv3515c 0.62 +0.6
3,951,840 - Rv3515c 0.62 +0.6
3,951,953 + Rv3515c 0.69 -0.4
3,951,953 + Rv3515c 0.69 +0.9
3,951,962 + Rv3515c 0.69 +2.0
3,951,962 + Rv3515c 0.69 +1.6
3,951,962 + Rv3515c 0.69 -0.2
3,951,963 - Rv3515c 0.69 +1.5
3,951,963 - Rv3515c 0.69 +0.7
3,952,093 + Rv3515c 0.77 -0.2
3,952,093 + Rv3515c 0.77 +0.1
3,952,093 + Rv3515c 0.77 -0.3
3,952,094 - Rv3515c 0.77 +0.3
3,952,094 - Rv3515c 0.77 +0.1
3,952,185 - Rv3515c 0.83 -0.6
3,952,290 - Rv3515c 0.89 +0.2
3,952,290 - Rv3515c 0.89 +1.3
3,952,356 + +0.1
3,952,374 - +0.9
3,952,453 - +0.2
3,952,453 - +0.6
3,952,797 - Rv3516 0.32 -0.9
3,953,130 + Rv3516 0.74 +0.5

Or see this region's nucleotide sequence