Experiment: 7H9 with Streptomycin 0.178 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv3514 and Rv3515c are separated by 560 nucleotides Rv3515c and Rv3516 are separated by 73 nucleotides Rv3516 and Rv3517 are separated by 95 nucleotides
Rv3514: Rv3514 - PE-PGRS family protein PE_PGRS57, at 3,945,794 to 3,950,263
Rv3514
Rv3515c: Rv3515c - Fatty-acid-CoA ligase FadD19 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase), at 3,950,824 to 3,952,470
Rv3515c
Rv3516: Rv3516 - Possible enoyl-CoA hydratase EchA19 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase), at 3,952,544 to 3,953,335
Rv3516
Rv3517: Rv3517 - Conserved hypothetical protein, at 3,953,431 to 3,954,270
Rv3517
Position (kb)
3950
3951
3952
3953 Strain fitness (log2 ratio)
-1
0
1
2 at 3950.108 kb on - strand at 3950.117 kb on - strand at 3950.125 kb on + strand at 3950.351 kb on + strand at 3950.402 kb on + strand at 3950.402 kb on + strand at 3950.402 kb on + strand at 3950.402 kb on + strand at 3950.403 kb on - strand at 3950.410 kb on - strand at 3950.611 kb on - strand at 3950.622 kb on - strand at 3950.690 kb on - strand at 3950.690 kb on - strand at 3950.720 kb on + strand at 3950.720 kb on + strand at 3950.720 kb on + strand at 3950.720 kb on + strand at 3951.839 kb on + strand, within Rv3515c at 3951.840 kb on - strand, within Rv3515c at 3951.953 kb on + strand, within Rv3515c at 3951.953 kb on + strand, within Rv3515c at 3951.962 kb on + strand, within Rv3515c at 3951.962 kb on + strand, within Rv3515c at 3951.962 kb on + strand, within Rv3515c at 3951.963 kb on - strand, within Rv3515c at 3951.963 kb on - strand, within Rv3515c at 3952.093 kb on + strand, within Rv3515c at 3952.093 kb on + strand, within Rv3515c at 3952.093 kb on + strand, within Rv3515c at 3952.094 kb on - strand, within Rv3515c at 3952.094 kb on - strand, within Rv3515c at 3952.185 kb on - strand, within Rv3515c at 3952.290 kb on - strand, within Rv3515c at 3952.290 kb on - strand, within Rv3515c at 3952.356 kb on + strand at 3952.374 kb on - strand at 3952.453 kb on - strand at 3952.453 kb on - strand at 3952.797 kb on - strand, within Rv3516 at 3953.130 kb on + strand, within Rv3516
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Streptomycin 0.178 ug/mL remove 3,950,108 - +0.3 3,950,117 - +0.6 3,950,125 + +0.4 3,950,351 + +0.1 3,950,402 + +0.4 3,950,402 + -0.5 3,950,402 + -0.3 3,950,402 + +0.2 3,950,403 - +1.5 3,950,410 - +1.3 3,950,611 - +0.3 3,950,622 - +1.6 3,950,690 - +1.1 3,950,690 - +0.2 3,950,720 + +2.0 3,950,720 + -1.6 3,950,720 + +0.4 3,950,720 + +0.1 3,951,839 + Rv3515c 0.62 +0.6 3,951,840 - Rv3515c 0.62 +0.6 3,951,953 + Rv3515c 0.69 -0.4 3,951,953 + Rv3515c 0.69 +0.9 3,951,962 + Rv3515c 0.69 +2.0 3,951,962 + Rv3515c 0.69 +1.6 3,951,962 + Rv3515c 0.69 -0.2 3,951,963 - Rv3515c 0.69 +1.5 3,951,963 - Rv3515c 0.69 +0.7 3,952,093 + Rv3515c 0.77 -0.2 3,952,093 + Rv3515c 0.77 +0.1 3,952,093 + Rv3515c 0.77 -0.3 3,952,094 - Rv3515c 0.77 +0.3 3,952,094 - Rv3515c 0.77 +0.1 3,952,185 - Rv3515c 0.83 -0.6 3,952,290 - Rv3515c 0.89 +0.2 3,952,290 - Rv3515c 0.89 +1.3 3,952,356 + +0.1 3,952,374 - +0.9 3,952,453 - +0.2 3,952,453 - +0.6 3,952,797 - Rv3516 0.32 -0.9 3,953,130 + Rv3516 0.74 +0.5
Or see this region's nucleotide sequence