Experiment: 7H9 with Streptomycin 0.178 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv3011c and Rv3012c overlap by 4 nucleotides Rv3012c and Rv3013 are separated by 84 nucleotides Rv3013 and Rv3014c are separated by 73 nucleotides
Rv3011c: Rv3011c - Probable glutamyl-tRNA(GLN) amidotransferase (subunit A) GatA (Glu-ADT subunit A), at 3,369,950 to 3,371,434
Rv3011c
Rv3012c: Rv3012c - Probable glutamyl-tRNA(GLN) amidotransferase (subunit C) GatC (Glu-ADT subunit C), at 3,371,431 to 3,371,730
Rv3012c
Rv3013: Rv3013 - Conserved protein, at 3,371,815 to 3,372,471
Rv3013
Rv3014c: Rv3014c - DNA ligase [NAD dependent] LigA (polydeoxyribonucleotide synthase [NAD+]), at 3,372,545 to 3,374,620
Rv3014c
Position (kb)
3371
3372
3373 Strain fitness (log2 ratio)
-1
0
1 at 3371.743 kb on + strand at 3371.789 kb on + strand at 3371.790 kb on - strand at 3371.790 kb on - strand at 3371.824 kb on + strand at 3371.824 kb on + strand at 3371.824 kb on + strand at 3371.824 kb on + strand at 3371.825 kb on - strand at 3371.825 kb on - strand at 3371.828 kb on + strand at 3371.829 kb on - strand at 3372.336 kb on + strand, within Rv3013 at 3372.437 kb on - strand at 3372.437 kb on - strand at 3372.441 kb on - strand at 3372.505 kb on - strand at 3372.546 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Streptomycin 0.178 ug/mL remove 3,371,743 + +1.0 3,371,789 + -0.4 3,371,790 - +0.3 3,371,790 - +0.3 3,371,824 + +0.6 3,371,824 + +0.2 3,371,824 + -0.0 3,371,824 + +0.4 3,371,825 - +0.3 3,371,825 - -0.2 3,371,828 + -0.3 3,371,829 - -0.7 3,372,336 + Rv3013 0.79 -1.2 3,372,437 - -1.4 3,372,437 - +1.3 3,372,441 - +1.0 3,372,505 - -0.8 3,372,546 + -0.6
Or see this region's nucleotide sequence