Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv2752c

Experiment: 7H9 with Streptomycin 0.178 ug/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv2750 and Rv2751 are separated by 3 nucleotidesRv2751 and Rv2752c overlap by 14 nucleotidesRv2752c and Rv2753c are separated by 30 nucleotides Rv2750: Rv2750 - Probable dehydrogenase, at 3,062,816 to 3,063,634 Rv2750 Rv2751: Rv2751 - Conserved protein, at 3,063,638 to 3,064,528 Rv2751 Rv2752c: Rv2752c - Conserved hypothetical protein, at 3,064,515 to 3,066,191 Rv2752c Rv2753c: Rv2753c - Probable dihydrodipicolinate synthase DapA (DHDPS) (dihydrodipicolinate synthetase), at 3,066,222 to 3,067,124 Rv2753c Position (kb) 3064 3065 3066 3067Strain fitness (log2 ratio) -1 0 1at 3063.632 kb on + strandat 3063.810 kb on + strand, within Rv2751at 3063.811 kb on - strand, within Rv2751at 3064.248 kb on + strand, within Rv2751at 3064.249 kb on - strand, within Rv2751at 3064.257 kb on - strand, within Rv2751at 3064.311 kb on - strand, within Rv2751at 3064.369 kb on - strand, within Rv2751at 3064.517 kb on - strandat 3065.355 kb on + strand, within Rv2752cat 3065.491 kb on - strand, within Rv2752cat 3065.637 kb on + strand, within Rv2752cat 3065.829 kb on + strand, within Rv2752cat 3065.836 kb on + strand, within Rv2752cat 3066.184 kb on - strandat 3067.090 kb on - strandat 3067.149 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Streptomycin 0.178 ug/mL
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3,063,632 + +0.2
3,063,810 + Rv2751 0.19 +0.3
3,063,811 - Rv2751 0.19 +0.5
3,064,248 + Rv2751 0.68 +0.2
3,064,249 - Rv2751 0.69 -0.1
3,064,257 - Rv2751 0.69 -1.0
3,064,311 - Rv2751 0.76 -0.3
3,064,369 - Rv2751 0.82 -1.1
3,064,517 - +0.3
3,065,355 + Rv2752c 0.50 +0.7
3,065,491 - Rv2752c 0.58 +0.4
3,065,637 + Rv2752c 0.67 -1.3
3,065,829 + Rv2752c 0.78 +1.2
3,065,836 + Rv2752c 0.79 +0.6
3,066,184 - -0.5
3,067,090 - -0.2
3,067,149 + -0.3

Or see this region's nucleotide sequence