Experiment: 7H9 with Streptomycin 0.178 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv2498c and Rv2499c overlap by 4 nucleotides Rv2499c and Rv2500c overlap by 4 nucleotides Rv2500c and Rv2501c are separated by 4 nucleotides
Rv2498c: Rv2498c - Probable citrate (pro-3S)-lyase (beta subunit) CitE (citrase) (citratase) (citritase) (citridesmolase) (citrase aldolase), at 2,812,355 to 2,813,176
Rv2498c
Rv2499c: Rv2499c - Possible oxidase regulatory-related protein, at 2,813,173 to 2,813,730
Rv2499c
Rv2500c: Rv2500c - Possible acyl-CoA dehydrogenase FadE19 (MMGC), at 2,813,727 to 2,814,911
Rv2500c
Rv2501c: Rv2501c - Probable acetyl-/propionyl-coenzyme A carboxylase alpha chain (alpha subunit) AccA1: biotin carboxylase + biotin carboxyl carrier protein (BCCP), at 2,814,916 to 2,816,880
Rv2501c
Position (kb)
2813
2814
2815 Strain fitness (log2 ratio)
-1
0
1 at 2812.921 kb on + strand, within Rv2498c at 2812.921 kb on + strand, within Rv2498c at 2812.924 kb on - strand, within Rv2498c at 2812.969 kb on + strand, within Rv2498c at 2812.969 kb on + strand, within Rv2498c at 2813.600 kb on + strand, within Rv2499c at 2813.840 kb on + strand at 2814.272 kb on + strand, within Rv2500c at 2814.918 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Streptomycin 0.178 ug/mL remove 2,812,921 + Rv2498c 0.69 -0.3 2,812,921 + Rv2498c 0.69 -0.2 2,812,924 - Rv2498c 0.69 -0.3 2,812,969 + Rv2498c 0.75 +0.2 2,812,969 + Rv2498c 0.75 -0.8 2,813,600 + Rv2499c 0.77 +0.6 2,813,840 + -0.3 2,814,272 + Rv2500c 0.46 -0.8 2,814,918 - +0.6
Or see this region's nucleotide sequence