Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv2500c

Experiment: 7H9 with Streptomycin 0.178 ug/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv2498c and Rv2499c overlap by 4 nucleotidesRv2499c and Rv2500c overlap by 4 nucleotidesRv2500c and Rv2501c are separated by 4 nucleotides Rv2498c: Rv2498c - Probable citrate (pro-3S)-lyase (beta subunit) CitE (citrase) (citratase) (citritase) (citridesmolase) (citrase aldolase), at 2,812,355 to 2,813,176 Rv2498c Rv2499c: Rv2499c - Possible oxidase regulatory-related protein, at 2,813,173 to 2,813,730 Rv2499c Rv2500c: Rv2500c - Possible acyl-CoA dehydrogenase FadE19 (MMGC), at 2,813,727 to 2,814,911 Rv2500c Rv2501c: Rv2501c - Probable acetyl-/propionyl-coenzyme A carboxylase alpha chain (alpha subunit) AccA1: biotin carboxylase + biotin carboxyl carrier protein (BCCP), at 2,814,916 to 2,816,880 Rv2501c Position (kb) 2813 2814 2815Strain fitness (log2 ratio) -1 0 1at 2812.921 kb on + strand, within Rv2498cat 2812.921 kb on + strand, within Rv2498cat 2812.924 kb on - strand, within Rv2498cat 2812.969 kb on + strand, within Rv2498cat 2812.969 kb on + strand, within Rv2498cat 2813.600 kb on + strand, within Rv2499cat 2813.840 kb on + strandat 2814.272 kb on + strand, within Rv2500cat 2814.918 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Streptomycin 0.178 ug/mL
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2,812,921 + Rv2498c 0.69 -0.3
2,812,921 + Rv2498c 0.69 -0.2
2,812,924 - Rv2498c 0.69 -0.3
2,812,969 + Rv2498c 0.75 +0.2
2,812,969 + Rv2498c 0.75 -0.8
2,813,600 + Rv2499c 0.77 +0.6
2,813,840 + -0.3
2,814,272 + Rv2500c 0.46 -0.8
2,814,918 - +0.6

Or see this region's nucleotide sequence