Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv2004c

Experiment: 7H9 with Streptomycin 0.178 ug/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv2003c and Rv2004c are separated by 57 nucleotidesRv2004c and Rv2005c are separated by 21 nucleotides Rv2003c: Rv2003c - Conserved hypothetical protein, at 2,248,563 to 2,249,420 Rv2003c Rv2004c: Rv2004c - Conserved protein, at 2,249,478 to 2,250,974 Rv2004c Rv2005c: Rv2005c - Universal stress protein family protein, at 2,250,996 to 2,251,883 Rv2005c Position (kb) 2249 2250 2251Strain fitness (log2 ratio) -2 -1 0 1at 2248.565 kb on - strandat 2248.593 kb on - strandat 2248.771 kb on + strand, within Rv2003cat 2248.823 kb on - strand, within Rv2003cat 2249.326 kb on - strand, within Rv2003cat 2249.504 kb on - strandat 2249.880 kb on + strand, within Rv2004cat 2250.140 kb on - strand, within Rv2004cat 2250.768 kb on - strand, within Rv2004cat 2251.578 kb on - strand, within Rv2005cat 2251.578 kb on - strand, within Rv2005cat 2251.578 kb on - strand, within Rv2005cat 2251.581 kb on + strand, within Rv2005cat 2251.597 kb on - strand, within Rv2005cat 2251.741 kb on - strand, within Rv2005cat 2251.741 kb on - strand, within Rv2005cat 2251.842 kb on + strandat 2251.877 kb on + strandat 2251.913 kb on - strandat 2251.913 kb on - strandat 2251.913 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Streptomycin 0.178 ug/mL
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2,248,565 - -0.0
2,248,593 - -0.5
2,248,771 + Rv2003c 0.24 +0.2
2,248,823 - Rv2003c 0.30 +0.3
2,249,326 - Rv2003c 0.89 -0.4
2,249,504 - +0.6
2,249,880 + Rv2004c 0.27 -1.8
2,250,140 - Rv2004c 0.44 +1.1
2,250,768 - Rv2004c 0.86 +0.2
2,251,578 - Rv2005c 0.66 -0.8
2,251,578 - Rv2005c 0.66 -0.1
2,251,578 - Rv2005c 0.66 +0.0
2,251,581 + Rv2005c 0.66 -0.0
2,251,597 - Rv2005c 0.68 -0.1
2,251,741 - Rv2005c 0.84 -1.3
2,251,741 - Rv2005c 0.84 -0.1
2,251,842 + -0.5
2,251,877 + -1.7
2,251,913 - -0.6
2,251,913 - +0.2
2,251,913 - +0.3

Or see this region's nucleotide sequence