Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv1085c

Experiment: 7H9 with Streptomycin 0.178 ug/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv1084 and Rv1085c are separated by 98 nucleotidesRv1085c and Rv1086 are separated by 110 nucleotides Rv1084: Rv1084 - Conserved protein, at 1,207,636 to 1,209,657 Rv1084 Rv1085c: Rv1085c - Possible hemolysin-like protein, at 1,209,756 to 1,210,484 Rv1085c Rv1086: Rv1086 - Short (C15) chain Z-isoprenyl diphosphate synthase (Z-FPP synthase) (Z-farnesyl diphosphate synthase) (Z-FPP synthetase) (Z-farnesyl diphosphate synthetase) (geranyltranstransferase) (farnesyl pyrophosphate synthetase), at 1,210,595 to 1,211,383 Rv1086 Position (kb) 1209 1210 1211Strain fitness (log2 ratio) -1 0 1 2at 1209.073 kb on + strand, within Rv1084at 1209.577 kb on + strandat 1209.577 kb on + strandat 1209.577 kb on + strandat 1209.578 kb on - strandat 1209.581 kb on + strandat 1209.867 kb on + strand, within Rv1085cat 1209.874 kb on + strand, within Rv1085cat 1209.875 kb on - strand, within Rv1085cat 1209.964 kb on + strand, within Rv1085cat 1209.994 kb on + strand, within Rv1085cat 1209.995 kb on - strand, within Rv1085cat 1210.133 kb on - strand, within Rv1085cat 1210.133 kb on - strand, within Rv1085cat 1210.133 kb on - strand, within Rv1085cat 1210.222 kb on + strand, within Rv1085cat 1210.264 kb on + strand, within Rv1085cat 1210.358 kb on - strand, within Rv1085cat 1210.535 kb on + strandat 1210.581 kb on + strandat 1210.581 kb on + strandat 1210.581 kb on + strandat 1210.582 kb on - strandat 1210.640 kb on + strandat 1211.335 kb on - strandat 1211.372 kb on + strandat 1211.401 kb on + strandat 1211.401 kb on + strandat 1211.401 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Streptomycin 0.178 ug/mL
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1,209,073 + Rv1084 0.71 +1.0
1,209,577 + -0.2
1,209,577 + +0.2
1,209,577 + +0.6
1,209,578 - +0.1
1,209,581 + +1.2
1,209,867 + Rv1085c 0.15 +0.8
1,209,874 + Rv1085c 0.16 -0.2
1,209,875 - Rv1085c 0.16 -0.6
1,209,964 + Rv1085c 0.29 +0.3
1,209,994 + Rv1085c 0.33 +0.6
1,209,995 - Rv1085c 0.33 +0.4
1,210,133 - Rv1085c 0.52 -0.5
1,210,133 - Rv1085c 0.52 +0.6
1,210,133 - Rv1085c 0.52 +0.8
1,210,222 + Rv1085c 0.64 +0.8
1,210,264 + Rv1085c 0.70 +0.5
1,210,358 - Rv1085c 0.83 +0.6
1,210,535 + +0.3
1,210,581 + +0.9
1,210,581 + -0.6
1,210,581 + +1.4
1,210,582 - +0.4
1,210,640 + -1.4
1,211,335 - -0.0
1,211,372 + +2.0
1,211,401 + +0.8
1,211,401 + +1.3
1,211,401 + +0.8

Or see this region's nucleotide sequence