Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv3857c

Experiment: 7H9 with para-aminosalicylic 0.086 ug/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv3856c and Rv3857c are separated by 8 nucleotidesRv3857c and Rv3858c are separated by 424 nucleotides Rv3856c: Rv3856c - Conserved hypothetical protein, at 4,328,401 to 4,329,408 Rv3856c Rv3857c: Rv3857c - Possible membrane protein, at 4,329,417 to 4,329,614 Rv3857c Rv3858c: Rv3858c - Probable NADH-dependent glutamate synthase (small subunit) GltD (L-glutamate synthase) (L-glutamate synthetase) (NADH-glutamate synthase) (glutamate synthase (NADH)) (GLTS beta chain) (NADPH-GOGAT), at 4,330,039 to 4,331,505 Rv3858c Position (kb) 4329 4330Strain fitness (log2 ratio) -1 0 1 2at 4328.572 kb on + strand, within Rv3856cat 4329.029 kb on + strand, within Rv3856cat 4329.040 kb on + strand, within Rv3856cat 4329.330 kb on - strandat 4329.371 kb on + strandat 4329.375 kb on - strandat 4329.495 kb on + strand, within Rv3857cat 4329.495 kb on + strand, within Rv3857cat 4329.565 kb on + strand, within Rv3857cat 4329.565 kb on + strand, within Rv3857cat 4329.566 kb on - strand, within Rv3857cat 4329.630 kb on - strandat 4329.630 kb on - strandat 4329.640 kb on + strandat 4329.641 kb on - strandat 4329.641 kb on - strandat 4329.735 kb on - strandat 4329.879 kb on + strandat 4329.929 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with para-aminosalicylic 0.086 ug/mL
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4,328,572 + Rv3856c 0.17 -0.2
4,329,029 + Rv3856c 0.62 -1.3
4,329,040 + Rv3856c 0.63 +2.0
4,329,330 - -0.9
4,329,371 + -0.2
4,329,375 - +0.6
4,329,495 + Rv3857c 0.39 +1.4
4,329,495 + Rv3857c 0.39 +0.0
4,329,565 + Rv3857c 0.75 -0.9
4,329,565 + Rv3857c 0.75 +0.5
4,329,566 - Rv3857c 0.75 +0.0
4,329,630 - +0.3
4,329,630 - -0.1
4,329,640 + -0.2
4,329,641 - -0.1
4,329,641 - -0.1
4,329,735 - -0.6
4,329,879 + -0.5
4,329,929 + +1.8

Or see this region's nucleotide sequence