Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv2159c

Experiment: 7H9 with para-aminosalicylic 0.086 ug/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv2158c and Rv2159c are separated by 22 nucleotidesRv2159c and Rv2160A overlap by 23 nucleotidesRv2160A and Rv2160c overlap by 617 nucleotidesRv2160c and Rv2161c are separated by 267 nucleotides Rv2158c: Rv2158c - Probable UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase MurE, at 2,419,001 to 2,420,608 Rv2158c Rv2159c: Rv2159c - Conserved protein, at 2,420,631 to 2,421,665 Rv2159c Rv2160A: Rv2160A - Conserved hypothetical protein, at 2,421,643 to 2,422,278 Rv2160A Rv2160c: Rv2160c - Conserved hypothetical protein, at 2,421,662 to 2,422,003 Rv2160c Rv2161c: Rv2161c - Conserved protein, at 2,422,271 to 2,423,137 Rv2161c Position (kb) 2420 2421 2422Strain fitness (log2 ratio) -1 0 1 2at 2421.006 kb on + strand, within Rv2159cat 2421.006 kb on + strand, within Rv2159cat 2421.007 kb on - strand, within Rv2159cat 2421.311 kb on - strand, within Rv2159cat 2421.311 kb on - strand, within Rv2159cat 2421.311 kb on - strand, within Rv2159cat 2421.311 kb on - strand, within Rv2159cat 2421.311 kb on - strand, within Rv2159cat 2421.388 kb on + strand, within Rv2159cat 2421.598 kb on + strandat 2421.599 kb on - strandat 2421.992 kb on + strandat 2422.447 kb on - strand, within Rv2161cat 2422.447 kb on - strand, within Rv2161c

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with para-aminosalicylic 0.086 ug/mL
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2,421,006 + Rv2159c 0.36 +0.4
2,421,006 + Rv2159c 0.36 +0.0
2,421,007 - Rv2159c 0.36 +1.8
2,421,311 - Rv2159c 0.66 +0.6
2,421,311 - Rv2159c 0.66 +0.0
2,421,311 - Rv2159c 0.66 +0.7
2,421,311 - Rv2159c 0.66 -0.0
2,421,311 - Rv2159c 0.66 +1.3
2,421,388 + Rv2159c 0.73 +0.4
2,421,598 + -0.1
2,421,599 - +0.2
2,421,992 + +0.1
2,422,447 - Rv2161c 0.20 -0.6
2,422,447 - Rv2161c 0.20 -0.5

Or see this region's nucleotide sequence