Experiment: 7H9 with Ofloxacin 0.8 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv3319 and Rv3320c are separated by 78 nucleotides Rv3320c and Rv3321c are separated by 3 nucleotides Rv3321c and Rv3322c are separated by 121 nucleotides Rv3322c and Rv3323c overlap by 4 nucleotides
Rv3319: Rv3319 - Probable succinate dehydrogenase (iron-sulphur protein subunit) SdhB (succinic dehydrogenase) (fumarate reductase) (fumarate dehydrogenase) (fumaric hydrogenase), at 3,706,772 to 3,707,563
Rv3319
Rv3320c: Rv3320c - Possible toxin VapC44. Contains PIN domain., at 3,707,642 to 3,708,070
Rv3320c
Rv3321c: Rv3321c - Possible antitoxin VapB44, at 3,708,074 to 3,708,316
Rv3321c
Rv3322c: Rv3322c - Possible methyltransferase, at 3,708,438 to 3,709,052
Rv3322c
Rv3323c: Rv3323c - Probable MoaD-MoaE fusion protein MoaX, at 3,709,049 to 3,709,714
Rv3323c
Position (kb)
3708
3709 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 3707.195 kb on + strand, within Rv3319 at 3707.213 kb on + strand, within Rv3319 at 3707.280 kb on - strand, within Rv3319 at 3707.354 kb on + strand, within Rv3319 at 3707.751 kb on + strand, within Rv3320c at 3708.204 kb on - strand, within Rv3321c at 3708.284 kb on + strand, within Rv3321c at 3708.285 kb on - strand, within Rv3321c at 3708.439 kb on + strand at 3708.439 kb on + strand at 3708.440 kb on - strand at 3708.440 kb on - strand at 3708.440 kb on - strand at 3708.440 kb on - strand at 3708.440 kb on - strand at 3708.440 kb on - strand at 3708.440 kb on - strand at 3708.440 kb on - strand at 3708.501 kb on - strand, within Rv3322c at 3708.516 kb on + strand, within Rv3322c at 3708.516 kb on + strand, within Rv3322c at 3708.630 kb on + strand, within Rv3322c at 3708.630 kb on + strand, within Rv3322c at 3708.630 kb on + strand, within Rv3322c at 3708.630 kb on + strand, within Rv3322c at 3708.630 kb on + strand, within Rv3322c at 3708.669 kb on + strand, within Rv3322c
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Ofloxacin 0.8 ug/mL remove 3,707,195 + Rv3319 0.53 -1.5 3,707,213 + Rv3319 0.56 -0.5 3,707,280 - Rv3319 0.64 -0.3 3,707,354 + Rv3319 0.73 -0.7 3,707,751 + Rv3320c 0.25 -2.7 3,708,204 - Rv3321c 0.53 -2.1 3,708,284 + Rv3321c 0.86 -0.9 3,708,285 - Rv3321c 0.87 -0.5 3,708,439 + +0.7 3,708,439 + -0.6 3,708,440 - -1.5 3,708,440 - +0.4 3,708,440 - -0.5 3,708,440 - +4.0 3,708,440 - -1.1 3,708,440 - -1.6 3,708,440 - -0.5 3,708,440 - +0.1 3,708,501 - Rv3322c 0.10 +0.4 3,708,516 + Rv3322c 0.13 -0.1 3,708,516 + Rv3322c 0.13 +0.5 3,708,630 + Rv3322c 0.31 -0.4 3,708,630 + Rv3322c 0.31 -0.5 3,708,630 + Rv3322c 0.31 -2.6 3,708,630 + Rv3322c 0.31 -1.2 3,708,630 + Rv3322c 0.31 +3.5 3,708,669 + Rv3322c 0.38 -2.1
Or see this region's nucleotide sequence