Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv3321c

Experiment: 7H9 with Ofloxacin 0.8 ug/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv3319 and Rv3320c are separated by 78 nucleotidesRv3320c and Rv3321c are separated by 3 nucleotidesRv3321c and Rv3322c are separated by 121 nucleotidesRv3322c and Rv3323c overlap by 4 nucleotides Rv3319: Rv3319 - Probable succinate dehydrogenase (iron-sulphur protein subunit) SdhB (succinic dehydrogenase) (fumarate reductase) (fumarate dehydrogenase) (fumaric hydrogenase), at 3,706,772 to 3,707,563 Rv3319 Rv3320c: Rv3320c - Possible toxin VapC44. Contains PIN domain., at 3,707,642 to 3,708,070 Rv3320c Rv3321c: Rv3321c - Possible antitoxin VapB44, at 3,708,074 to 3,708,316 Rv3321c Rv3322c: Rv3322c - Possible methyltransferase, at 3,708,438 to 3,709,052 Rv3322c Rv3323c: Rv3323c - Probable MoaD-MoaE fusion protein MoaX, at 3,709,049 to 3,709,714 Rv3323c Position (kb) 3708 3709Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 3707.195 kb on + strand, within Rv3319at 3707.213 kb on + strand, within Rv3319at 3707.280 kb on - strand, within Rv3319at 3707.354 kb on + strand, within Rv3319at 3707.751 kb on + strand, within Rv3320cat 3708.204 kb on - strand, within Rv3321cat 3708.284 kb on + strand, within Rv3321cat 3708.285 kb on - strand, within Rv3321cat 3708.439 kb on + strandat 3708.439 kb on + strandat 3708.440 kb on - strandat 3708.440 kb on - strandat 3708.440 kb on - strandat 3708.440 kb on - strandat 3708.440 kb on - strandat 3708.440 kb on - strandat 3708.440 kb on - strandat 3708.440 kb on - strandat 3708.501 kb on - strand, within Rv3322cat 3708.516 kb on + strand, within Rv3322cat 3708.516 kb on + strand, within Rv3322cat 3708.630 kb on + strand, within Rv3322cat 3708.630 kb on + strand, within Rv3322cat 3708.630 kb on + strand, within Rv3322cat 3708.630 kb on + strand, within Rv3322cat 3708.630 kb on + strand, within Rv3322cat 3708.669 kb on + strand, within Rv3322c

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Ofloxacin 0.8 ug/mL
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3,707,195 + Rv3319 0.53 -1.5
3,707,213 + Rv3319 0.56 -0.5
3,707,280 - Rv3319 0.64 -0.3
3,707,354 + Rv3319 0.73 -0.7
3,707,751 + Rv3320c 0.25 -2.7
3,708,204 - Rv3321c 0.53 -2.1
3,708,284 + Rv3321c 0.86 -0.9
3,708,285 - Rv3321c 0.87 -0.5
3,708,439 + +0.7
3,708,439 + -0.6
3,708,440 - -1.5
3,708,440 - +0.4
3,708,440 - -0.5
3,708,440 - +4.0
3,708,440 - -1.1
3,708,440 - -1.6
3,708,440 - -0.5
3,708,440 - +0.1
3,708,501 - Rv3322c 0.10 +0.4
3,708,516 + Rv3322c 0.13 -0.1
3,708,516 + Rv3322c 0.13 +0.5
3,708,630 + Rv3322c 0.31 -0.4
3,708,630 + Rv3322c 0.31 -0.5
3,708,630 + Rv3322c 0.31 -2.6
3,708,630 + Rv3322c 0.31 -1.2
3,708,630 + Rv3322c 0.31 +3.5
3,708,669 + Rv3322c 0.38 -2.1

Or see this region's nucleotide sequence