Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv3000

Experiment: 7H9 with Ofloxacin 0.8 ug/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv2999 and Rv3000 are separated by 44 nucleotidesRv3000 and Rv3001c are separated by 313 nucleotides Rv2999: Rv2999 - Probable conserved lipoprotein LppY, at 3,357,602 to 3,358,567 Rv2999 Rv3000: Rv3000 - Possible conserved transmembrane protein, at 3,358,612 to 3,359,271 Rv3000 Rv3001c: Rv3001c - Probable KETOL-acid reductoisomerase IlvC (acetohydroxy-acid isomeroreductase) (alpha-keto-beta-hydroxylacil reductoisomerase), at 3,359,585 to 3,360,586 Rv3001c Position (kb) 3358 3359 3360Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 3357.660 kb on + strandat 3357.661 kb on - strandat 3358.478 kb on + strandat 3358.479 kb on - strandat 3358.565 kb on + strandat 3359.153 kb on - strand, within Rv3000at 3359.586 kb on + strandat 3359.587 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Ofloxacin 0.8 ug/mL
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3,357,660 + -3.5
3,357,661 - -0.4
3,358,478 + +1.9
3,358,479 - +0.0
3,358,565 + -0.4
3,359,153 - Rv3000 0.82 -1.0
3,359,586 + -1.0
3,359,587 - -0.3

Or see this region's nucleotide sequence