Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv1680

Experiment: 7H9 with Ofloxacin 0.8 ug/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv1679 and Rv1680 are separated by 8 nucleotidesRv1680 and Rv1681 overlap by 4 nucleotides Rv1679: Rv1679 - Possible acyl-CoA dehydrogenase FadE16, at 1,903,299 to 1,904,420 Rv1679 Rv1680: Rv1680 - Hypothetical protein, at 1,904,429 to 1,905,253 Rv1680 Rv1681: Rv1681 - Possible molybdopterin biosynthesis protein MoeX, at 1,905,250 to 1,906,242 Rv1681 Position (kb) 1904 1905 1906Strain fitness (log2 ratio) -2 -1 0 1at 1903.759 kb on - strand, within Rv1679at 1903.923 kb on + strand, within Rv1679at 1904.385 kb on + strandat 1904.565 kb on - strand, within Rv1680at 1904.817 kb on - strand, within Rv1680at 1905.070 kb on + strand, within Rv1680at 1905.089 kb on + strand, within Rv1680at 1905.258 kb on - strandat 1905.771 kb on + strand, within Rv1681at 1905.771 kb on + strand, within Rv1681at 1905.772 kb on - strand, within Rv1681at 1905.772 kb on - strand, within Rv1681at 1905.860 kb on + strand, within Rv1681at 1905.861 kb on - strand, within Rv1681at 1905.861 kb on - strand, within Rv1681at 1906.094 kb on - strand, within Rv1681at 1906.175 kb on + strandat 1906.175 kb on + strandat 1906.176 kb on - strandat 1906.176 kb on - strandat 1906.176 kb on - strandat 1906.240 kb on + strandat 1906.240 kb on + strandat 1906.241 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Ofloxacin 0.8 ug/mL
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1,903,759 - Rv1679 0.41 -0.2
1,903,923 + Rv1679 0.56 -1.4
1,904,385 + +0.3
1,904,565 - Rv1680 0.16 -0.6
1,904,817 - Rv1680 0.47 -0.1
1,905,070 + Rv1680 0.78 -1.3
1,905,089 + Rv1680 0.80 -1.1
1,905,258 - -0.6
1,905,771 + Rv1681 0.52 +0.4
1,905,771 + Rv1681 0.52 -0.0
1,905,772 - Rv1681 0.53 +0.5
1,905,772 - Rv1681 0.53 -0.1
1,905,860 + Rv1681 0.61 -0.7
1,905,861 - Rv1681 0.62 -0.5
1,905,861 - Rv1681 0.62 -0.5
1,906,094 - Rv1681 0.85 -1.7
1,906,175 + -0.9
1,906,175 + -0.1
1,906,176 - +0.9
1,906,176 - +0.7
1,906,176 - -1.1
1,906,240 + -2.4
1,906,240 + -0.4
1,906,241 - +0.1

Or see this region's nucleotide sequence