Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv0694

Experiment: 7H9 with Ofloxacin 0.8 ug/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv0693 and Rv0694 are separated by 2 nucleotidesRv0694 and Rv0695 are separated by 189 nucleotidesRv0695 and Rv0696 are separated by 48 nucleotides Rv0693: Rv0693 - Probable coenzyme PQQ synthesis protein E PqqE (coenzyme PQQ synthesis protein III), at 792,157 to 793,332 Rv0693 Rv0694: Rv0694 - Possible L-lactate dehydrogenase (cytochrome) LldD1, at 793,335 to 794,525 Rv0694 Rv0695: Rv0695 - Conserved hypothetical protein, at 794,715 to 795,470 Rv0695 Rv0696: Rv0696 - Probable membrane sugar transferase, at 795,519 to 796,931 Rv0696 Position (kb) 793 794 795Strain fitness (log2 ratio) -3 -2 -1 0 1at 792.358 kb on + strand, within Rv0693at 792.419 kb on - strand, within Rv0693at 792.761 kb on + strand, within Rv0693at 792.955 kb on + strand, within Rv0693at 793.103 kb on - strand, within Rv0693at 793.955 kb on - strand, within Rv0694at 793.955 kb on - strand, within Rv0694at 794.337 kb on + strand, within Rv0694at 794.337 kb on + strand, within Rv0694at 794.338 kb on - strand, within Rv0694at 794.338 kb on - strand, within Rv0694at 794.696 kb on - strandat 794.783 kb on - strandat 794.852 kb on - strand, within Rv0695at 794.852 kb on - strand, within Rv0695at 795.130 kb on + strand, within Rv0695

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Ofloxacin 0.8 ug/mL
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792,358 + Rv0693 0.17 +0.5
792,419 - Rv0693 0.22 +0.1
792,761 + Rv0693 0.51 -3.1
792,955 + Rv0693 0.68 +0.1
793,103 - Rv0693 0.80 -1.5
793,955 - Rv0694 0.52 -0.5
793,955 - Rv0694 0.52 -1.0
794,337 + Rv0694 0.84 -0.8
794,337 + Rv0694 0.84 -1.9
794,338 - Rv0694 0.84 -0.1
794,338 - Rv0694 0.84 +0.2
794,696 - +1.1
794,783 - -2.2
794,852 - Rv0695 0.18 -1.2
794,852 - Rv0695 0.18 -0.4
795,130 + Rv0695 0.55 -0.6

Or see this region's nucleotide sequence