Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv0649

Experiment: 7H9 with Ofloxacin 0.8 ug/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv0648 and Rv0649 overlap by 4 nucleotidesRv0649 and Rv0650 overlap by 1 nucleotides Rv0648: Rv0648 - Alpha-mannosidase, at 742,719 to 746,366 Rv0648 Rv0649: Rv0649 - Possible malonyl CoA-acyl carrier protein transacylase FabD2 (MCT), at 746,363 to 747,037 Rv0649 Rv0650: Rv0650 - Possible sugar kinase, at 747,037 to 747,945 Rv0650 Position (kb) 746 747 748Strain fitness (log2 ratio) -3 -2 -1 0 1at 745.456 kb on + strand, within Rv0648at 745.456 kb on + strand, within Rv0648at 745.456 kb on + strand, within Rv0648at 745.457 kb on - strand, within Rv0648at 745.590 kb on - strand, within Rv0648at 745.651 kb on - strand, within Rv0648at 746.058 kb on + strandat 746.058 kb on + strandat 746.058 kb on + strandat 746.059 kb on - strandat 746.225 kb on - strandat 746.334 kb on + strandat 746.789 kb on + strand, within Rv0649at 746.789 kb on + strand, within Rv0649at 746.790 kb on - strand, within Rv0649at 746.790 kb on - strand, within Rv0649at 746.790 kb on - strand, within Rv0649at 746.988 kb on - strandat 747.246 kb on + strand, within Rv0650at 747.254 kb on + strand, within Rv0650at 747.824 kb on + strand, within Rv0650at 747.824 kb on + strand, within Rv0650at 747.943 kb on + strandat 748.010 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Ofloxacin 0.8 ug/mL
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745,456 + Rv0648 0.75 -0.2
745,456 + Rv0648 0.75 -0.5
745,456 + Rv0648 0.75 -0.4
745,457 - Rv0648 0.75 +0.4
745,590 - Rv0648 0.79 +0.0
745,651 - Rv0648 0.80 -2.9
746,058 + -0.4
746,058 + +0.2
746,058 + -1.2
746,059 - -0.3
746,225 - -0.9
746,334 + -0.2
746,789 + Rv0649 0.63 +0.7
746,789 + Rv0649 0.63 -1.0
746,790 - Rv0649 0.63 -0.5
746,790 - Rv0649 0.63 -1.2
746,790 - Rv0649 0.63 -1.9
746,988 - +0.0
747,246 + Rv0650 0.23 +1.1
747,254 + Rv0650 0.24 -0.3
747,824 + Rv0650 0.87 -2.7
747,824 + Rv0650 0.87 -0.4
747,943 + -2.1
748,010 - +0.3

Or see this region's nucleotide sequence