Experiment: 7H9 with Ofloxacin 0.8 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv0331 and Rv0332 are separated by 74 nucleotides Rv0332 and Rv0333 are separated by 26 nucleotides Rv0333 and Rv0334 are separated by 29 nucleotides Rv0334 and Rv0335c are separated by 10 nucleotides
Rv0331: Rv0331 - Possible dehydrogenase/reductase, at 396,201 to 397,367
Rv0331
Rv0332: Rv0332 - Conserved protein, at 397,442 to 398,227
Rv0332
Rv0333: Rv0333 - Unknown protein, at 398,254 to 398,628
Rv0333
Rv0334: Rv0334 - Alpha-D-glucose-1-phosphate thymidylyltransferase RmlA (dTDP-glucose synthase) (dTDP-glucose pyrophosphorylase), at 398,658 to 399,524
Rv0334
Rv0335c: Rv0335c - PE family protein PE6, at 399,535 to 400,050
Rv0335c
Position (kb) 398
399 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 397.391 kb on - strand at 397.402 kb on + strand at 397.456 kb on + strand at 397.459 kb on + strand at 397.478 kb on + strand at 397.490 kb on + strand at 397.584 kb on + strand, within Rv0332 at 397.585 kb on - strand, within Rv0332 at 397.718 kb on + strand, within Rv0332 at 397.719 kb on - strand, within Rv0332 at 397.993 kb on - strand, within Rv0332 at 397.993 kb on - strand, within Rv0332 at 398.337 kb on + strand, within Rv0333 at 398.354 kb on + strand, within Rv0333 at 398.375 kb on + strand, within Rv0333 at 398.646 kb on + strand at 398.657 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Ofloxacin 0.8 ug/mL remove 397,391 - -2.9 397,402 + +3.1 397,456 + +1.9 397,459 + -0.5 397,478 + -1.3 397,490 + +1.5 397,584 + Rv0332 0.18 -0.2 397,585 - Rv0332 0.18 +0.5 397,718 + Rv0332 0.35 -2.9 397,719 - Rv0332 0.35 +0.0 397,993 - Rv0332 0.70 -0.1 397,993 - Rv0332 0.70 +1.3 398,337 + Rv0333 0.22 -1.1 398,354 + Rv0333 0.27 -1.1 398,375 + Rv0333 0.32 -0.6 398,646 + -0.6 398,657 + -2.7
Or see this region's nucleotide sequence