Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv0253

Experiment: 7H9 with Ofloxacin 0.8 ug/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv0252 and Rv0253 are separated by 25 nucleotidesRv0253 and Rv0254c are separated by 15 nucleotidesRv0254c and Rv0255c are separated by 24 nucleotides Rv0252: Rv0252 - Probable nitrite reductase [NAD(P)H] large subunit [FAD flavoprotein] NirB, at 302,866 to 305,427 Rv0252 Rv0253: Rv0253 - Probable nitrite reductase [NAD(P)H] small subunit NirD, at 305,453 to 305,809 Rv0253 Rv0254c: Rv0254c - Probable bifunctional cobalamin biosynthesis protein CobU: cobinamide kinase + cobinamide phosphate guanylyltransferase, at 305,825 to 306,349 Rv0254c Rv0255c: Rv0255c - Probable cobyric acid synthase CobQ1, at 306,374 to 307,858 Rv0255c Position (kb) 305 306Strain fitness (log2 ratio) -1 0 1 2at 304.597 kb on + strand, within Rv0252at 304.684 kb on + strand, within Rv0252at 304.685 kb on - strand, within Rv0252at 304.796 kb on - strand, within Rv0252at 304.908 kb on - strand, within Rv0252at 304.925 kb on + strand, within Rv0252at 304.925 kb on + strand, within Rv0252at 304.925 kb on + strand, within Rv0252at 304.926 kb on - strand, within Rv0252at 305.018 kb on - strand, within Rv0252at 305.018 kb on - strand, within Rv0252at 305.060 kb on + strand, within Rv0252at 305.123 kb on - strand, within Rv0252at 305.266 kb on + strandat 305.267 kb on - strandat 305.406 kb on + strandat 305.406 kb on + strandat 305.479 kb on - strandat 305.753 kb on + strand, within Rv0253at 305.808 kb on - strandat 305.861 kb on + strandat 305.861 kb on + strandat 306.039 kb on + strand, within Rv0254c

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Ofloxacin 0.8 ug/mL
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304,597 + Rv0252 0.68 -0.1
304,684 + Rv0252 0.71 +0.0
304,685 - Rv0252 0.71 +0.8
304,796 - Rv0252 0.75 -0.2
304,908 - Rv0252 0.80 -0.7
304,925 + Rv0252 0.80 +0.9
304,925 + Rv0252 0.80 +0.6
304,925 + Rv0252 0.80 -0.8
304,926 - Rv0252 0.80 -0.7
305,018 - Rv0252 0.84 -0.4
305,018 - Rv0252 0.84 -0.2
305,060 + Rv0252 0.86 +2.2
305,123 - Rv0252 0.88 -0.7
305,266 + +0.6
305,267 - +0.2
305,406 + -0.2
305,406 + -0.2
305,479 - -0.3
305,753 + Rv0253 0.84 -0.8
305,808 - -0.0
305,861 + -0.3
305,861 + -0.3
306,039 + Rv0254c 0.41 -0.4

Or see this region's nucleotide sequence