Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv3254

Experiment: 7H9 with Ofloxacin 0.8 ug/mL

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	7H9 with Ofloxacin 0.8 ug/mL
3,632,689	+		Rv3253c	0.40	+1.6
3,632,689	+		Rv3253c	0.40	+0.3
3,632,689	+		Rv3253c	0.40	-1.2
3,632,689	+		Rv3253c	0.40	+0.0
3,632,689	+		Rv3253c	0.40	+0.2
3,632,690	-		Rv3253c	0.40	-2.1
3,632,690	-		Rv3253c	0.40	-1.4
3,632,729	-		Rv3253c	0.42	-0.3
3,632,873	-		Rv3253c	0.52	+0.3
3,632,881	+		Rv3253c	0.53	-0.1
3,632,882	-		Rv3253c	0.53	-0.1
3,632,882	-		Rv3253c	0.53	+0.3
3,632,977	+		Rv3253c	0.59	-0.1
3,632,995	+		Rv3253c	0.60	-0.4
3,632,995	+		Rv3253c	0.60	-2.1
3,632,996	-		Rv3253c	0.60	-0.3
3,632,996	-		Rv3253c	0.60	-0.4
3,633,293	-		Rv3253c	0.80	-0.3
3,633,302	+		Rv3253c	0.81	-0.5
3,633,302	+		Rv3253c	0.81	-2.0
3,633,302	+		Rv3253c	0.81	+1.2
3,633,302	+		Rv3253c	0.81	-0.4
3,633,302	+		Rv3253c	0.81	-2.8
3,633,304	+		Rv3253c	0.81	-0.5
3,633,304	+		Rv3253c	0.81	+0.0
3,633,305	-		Rv3253c	0.81	-0.2
3,633,447	+				+0.5
3,633,620	+				-1.0
3,633,620	+				+0.1
3,633,620	+				-0.9
3,633,628	-				-0.9
3,633,924	-		Rv3254	0.18	-0.6
3,633,928	-		Rv3254	0.18	-0.0
3,633,965	+		Rv3254	0.21	-0.2
3,633,991	-		Rv3254	0.23	+1.4
3,633,991	-		Rv3254	0.23	-0.2
3,634,172	-		Rv3254	0.36	+0.1
3,634,284	+		Rv3254	0.44	+1.9
3,634,386	+		Rv3254	0.51	+0.1
3,634,387	-		Rv3254	0.51	+0.3
3,634,688	-		Rv3254	0.73	+0.8
3,634,718	+		Rv3254	0.75	+1.3
3,634,718	+		Rv3254	0.75	-1.9
3,634,770	+		Rv3254	0.79	+0.2
3,634,771	-		Rv3254	0.79	+0.1
3,634,950	+				-0.1
3,634,951	-				-0.3