Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv2976c

Experiment: 7H9 with Ofloxacin 0.8 ug/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv2974c and Rv2975c overlap by 4 nucleotidesRv2975c and Rv2976c are separated by 458 nucleotidesRv2976c and Rv2977c are separated by 32 nucleotides Rv2974c: Rv2974c - Conserved hypothetical alanine rich protein, at 3,329,949 to 3,331,361 Rv2974c Rv2975c: Rv2975c - Conserved hypothetical protein, at 3,331,358 to 3,331,612 Rv2975c Rv2976c: Rv2976c - Probable uracil-DNA glycosylase Ung (UDG), at 3,332,071 to 3,332,754 Rv2976c Rv2977c: Rv2977c - Probable thiamine-monophosphate kinase ThiL (thiamine-phosphate kinase), at 3,332,787 to 3,333,788 Rv2977c Position (kb) 3332 3333Strain fitness (log2 ratio) -1 0 1 2at 3331.166 kb on + strand, within Rv2974cat 3331.442 kb on + strand, within Rv2975cat 3331.442 kb on + strand, within Rv2975cat 3331.613 kb on + strandat 3331.613 kb on + strandat 3331.614 kb on - strandat 3331.614 kb on - strandat 3331.614 kb on - strandat 3331.668 kb on + strandat 3331.669 kb on - strandat 3332.518 kb on - strand, within Rv2976cat 3332.631 kb on - strand, within Rv2976cat 3332.770 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Ofloxacin 0.8 ug/mL
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3,331,166 + Rv2974c 0.86 -1.1
3,331,442 + Rv2975c 0.33 -0.4
3,331,442 + Rv2975c 0.33 +1.8
3,331,613 + +0.4
3,331,613 + -0.7
3,331,614 - -0.2
3,331,614 - -1.4
3,331,614 - -0.2
3,331,668 + -1.3
3,331,669 - +0.3
3,332,518 - Rv2976c 0.65 -0.3
3,332,631 - Rv2976c 0.82 -0.5
3,332,770 + -1.1

Or see this region's nucleotide sequence