Experiment: 7H9 with Ofloxacin 0.8 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv2230c and Rv2231c overlap by 4 nucleotides Rv2231c and Rv2231A are separated by 36 nucleotides Rv2231A and Rv2231B are separated by 45 nucleotides Rv2231B and Rv2232 overlap by 106 nucleotides
Rv2230c: Rv2230c - Conserved protein, at 2,503,469 to 2,504,608
Rv2230c
Rv2231c: Rv2231c - Possible aminotransferase CobC, at 2,504,605 to 2,505,699
Rv2231c
Rv2231A: Rv2231A - Possible toxin VapC16, at 2,505,736 to 2,506,161
Rv2231A
Rv2231B: Rv2231B - Possible antitoxin VapB16, at 2,506,207 to 2,506,383
Rv2231B
Rv2232: Rv2232 - Protein tyrosine kinase transcriptional regulatory protein PtkA, at 2,506,278 to 2,507,153
Rv2232
Position (kb)
2504
2505
2506 Strain fitness (log2 ratio)
-1
0
1
2 at 2504.191 kb on - strand, within Rv2230c at 2505.009 kb on - strand, within Rv2231c at 2505.641 kb on - strand at 2505.641 kb on - strand at 2505.664 kb on + strand at 2505.665 kb on - strand at 2505.747 kb on + strand at 2505.748 kb on - strand at 2505.748 kb on - strand at 2505.915 kb on - strand, within Rv2231A at 2505.915 kb on - strand, within Rv2231A at 2506.076 kb on - strand, within Rv2231A at 2506.371 kb on - strand at 2506.570 kb on + strand, within Rv2232
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Ofloxacin 0.8 ug/mL remove 2,504,191 - Rv2230c 0.63 -0.6 2,505,009 - Rv2231c 0.37 -1.0 2,505,641 - +2.6 2,505,641 - +1.8 2,505,664 + +0.0 2,505,665 - -0.0 2,505,747 + -1.1 2,505,748 - -1.6 2,505,748 - +0.0 2,505,915 - Rv2231A 0.42 -0.2 2,505,915 - Rv2231A 0.42 +1.5 2,506,076 - Rv2231A 0.80 -1.3 2,506,371 - +0.2 2,506,570 + Rv2232 0.33 +0.8
Or see this region's nucleotide sequence