Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv1422

Experiment: 7H9 with Ethionamide 0.1 ug/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv1420 and Rv1421 overlap by 4 nucleotidesRv1421 and Rv1422 overlap by 4 nucleotidesRv1422 and Rv1423 overlap by 4 nucleotidesRv1423 and Rv1424c are separated by 9 nucleotides Rv1420: Rv1420 - Probable excinuclease ABC (subunit C-nuclease) UvrC, at 1,594,042 to 1,595,982 Rv1420 Rv1421: Rv1421 - Conserved protein, at 1,595,979 to 1,596,884 Rv1421 Rv1422: Rv1422 - Conserved hypothetical protein, at 1,596,881 to 1,597,909 Rv1422 Rv1423: Rv1423 - Probable transcriptional regulatory protein WhiA, at 1,597,906 to 1,598,883 Rv1423 Rv1424c: Rv1424c - Possible membrane protein, at 1,598,893 to 1,599,654 Rv1424c Position (kb) 1596 1597 1598Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1596.105 kb on + strand, within Rv1421at 1596.400 kb on + strand, within Rv1421at 1596.710 kb on - strand, within Rv1421at 1596.753 kb on - strand, within Rv1421at 1596.783 kb on - strand, within Rv1421at 1596.960 kb on - strandat 1597.485 kb on + strand, within Rv1422at 1597.486 kb on - strand, within Rv1422at 1597.770 kb on + strand, within Rv1422at 1598.895 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Ethionamide 0.1 ug/mL
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1,596,105 + Rv1421 0.14 -1.0
1,596,400 + Rv1421 0.46 -0.4
1,596,710 - Rv1421 0.81 +0.1
1,596,753 - Rv1421 0.85 -2.7
1,596,783 - Rv1421 0.89 +0.3
1,596,960 - -0.3
1,597,485 + Rv1422 0.59 +1.7
1,597,486 - Rv1422 0.59 -0.0
1,597,770 + Rv1422 0.86 +0.9
1,598,895 - +3.0

Or see this region's nucleotide sequence