Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv3675

Experiment: 7H9 with Ethionamide 0.1 ug/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv3673c and Rv3674c overlap by 1 nucleotidesRv3674c and Rv3675 are separated by 107 nucleotidesRv3675 and Rv3676 are separated by 98 nucleotidesRv3676 and Rv3677c are separated by 105 nucleotides Rv3673c: Rv3673c - Possible membrane-anchored thioredoxin-like protein (thiol-disulfide interchange related protein), at 4,114,474 to 4,115,157 Rv3673c Rv3674c: Rv3674c - Probable endonuclease III Nth (DNA-(apurinic or apyrimidinic site)lyase) (AP lyase) (AP endonuclease class I) (endodeoxyribonuclease (apurinic or apyrimidinic)) (deoxyribonuclease (apurinic or apyrimidinic)), at 4,115,157 to 4,115,894 Rv3674c Rv3675: Rv3675 - Possible membrane protein, at 4,116,002 to 4,116,379 Rv3675 Rv3676: Rv3676 - Transcriptional regulatory protein Crp (Crp/Fnr-family), at 4,116,478 to 4,117,152 Rv3676 Rv3677c: Rv3677c - Possible hydrolase, at 4,117,258 to 4,118,052 Rv3677c Position (kb) 4116 4117Strain fitness (log2 ratio) -2 -1 0 1 2at 4115.158 kb on + strandat 4115.159 kb on - strandat 4115.575 kb on - strand, within Rv3674cat 4115.575 kb on - strand, within Rv3674cat 4115.575 kb on - strand, within Rv3674cat 4115.671 kb on + strand, within Rv3674cat 4115.672 kb on - strand, within Rv3674cat 4115.751 kb on + strand, within Rv3674cat 4115.802 kb on + strand, within Rv3674cat 4115.803 kb on - strand, within Rv3674cat 4115.957 kb on + strandat 4115.958 kb on - strandat 4116.007 kb on + strandat 4116.008 kb on - strandat 4116.167 kb on - strand, within Rv3675at 4116.167 kb on - strand, within Rv3675at 4116.167 kb on - strand, within Rv3675at 4116.167 kb on - strand, within Rv3675at 4116.304 kb on + strand, within Rv3675at 4116.405 kb on + strandat 4116.447 kb on + strandat 4117.184 kb on + strandat 4117.185 kb on - strandat 4117.185 kb on - strandat 4117.275 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Ethionamide 0.1 ug/mL
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4,115,158 + -1.1
4,115,159 - +0.0
4,115,575 - Rv3674c 0.57 -1.7
4,115,575 - Rv3674c 0.57 -0.8
4,115,575 - Rv3674c 0.57 -0.1
4,115,671 + Rv3674c 0.70 -1.3
4,115,672 - Rv3674c 0.70 -2.2
4,115,751 + Rv3674c 0.80 -0.4
4,115,802 + Rv3674c 0.87 -0.7
4,115,803 - Rv3674c 0.88 -2.1
4,115,957 + +0.8
4,115,958 - -0.5
4,116,007 + -0.6
4,116,008 - -0.7
4,116,167 - Rv3675 0.44 -0.1
4,116,167 - Rv3675 0.44 -0.1
4,116,167 - Rv3675 0.44 -1.5
4,116,167 - Rv3675 0.44 -0.7
4,116,304 + Rv3675 0.80 -1.3
4,116,405 + +2.4
4,116,447 + +0.1
4,117,184 + -1.0
4,117,185 - -1.1
4,117,185 - +0.7
4,117,275 - -0.2

Or see this region's nucleotide sequence