Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv2175c

Experiment: 7H9 with Ethionamide 0.1 ug/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv2174 and Rv2175c overlap by 14 nucleotidesRv2175c and Rv2176 are separated by 54 nucleotides Rv2174: Rv2174 - Alpha(1->6)mannosyltransferase. Possible conserved integral membrane protein., at 2,435,909 to 2,437,459 Rv2174 Rv2175c: Rv2175c - Conserved regulatory protein, at 2,437,446 to 2,437,886 Rv2175c Rv2176: Rv2176 - Probable transmembrane serine/threonine-protein kinase L PknL (protein kinase L) (STPK L), at 2,437,941 to 2,439,140 Rv2176 Position (kb) 2437 2438Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2437.400 kb on + strandat 2437.450 kb on + strandat 2437.450 kb on + strandat 2437.451 kb on - strandat 2437.451 kb on - strandat 2437.798 kb on + strand, within Rv2175cat 2437.799 kb on - strand, within Rv2175cat 2437.929 kb on + strandat 2437.929 kb on + strandat 2437.929 kb on + strandat 2437.930 kb on - strandat 2437.930 kb on - strandat 2437.995 kb on + strandat 2438.161 kb on + strand, within Rv2176at 2438.267 kb on - strand, within Rv2176at 2438.491 kb on - strand, within Rv2176at 2438.544 kb on + strand, within Rv2176at 2438.545 kb on - strand, within Rv2176at 2438.565 kb on + strand, within Rv2176at 2438.622 kb on + strand, within Rv2176at 2438.865 kb on - strand, within Rv2176

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Ethionamide 0.1 ug/mL
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2,437,400 + -0.7
2,437,450 + -0.3
2,437,450 + -3.1
2,437,451 - -1.2
2,437,451 - +0.9
2,437,798 + Rv2175c 0.80 +1.1
2,437,799 - Rv2175c 0.80 +0.2
2,437,929 + +0.0
2,437,929 + +0.3
2,437,929 + +0.7
2,437,930 - -1.2
2,437,930 - -1.4
2,437,995 + +1.8
2,438,161 + Rv2176 0.18 +0.3
2,438,267 - Rv2176 0.27 -0.4
2,438,491 - Rv2176 0.46 -0.2
2,438,544 + Rv2176 0.50 +1.1
2,438,545 - Rv2176 0.50 +0.3
2,438,565 + Rv2176 0.52 -0.1
2,438,622 + Rv2176 0.57 -0.9
2,438,865 - Rv2176 0.77 +1.5

Or see this region's nucleotide sequence