Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv3654c

Experiment: 7H9 with Isoniazid 0.025 ug/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv3652 and Rv3653 overlap by 7 nucleotidesRv3653 and Rv3654c are separated by 132 nucleotidesRv3654c and Rv3655c are separated by 8 nucleotidesRv3655c and Rv3656c are separated by 23 nucleotidesRv3656c and Rv3657c are separated by 9 nucleotides Rv3652: Rv3652 - PE-PGRS family-related protein PE_PGRS60, at 4,093,632 to 4,093,946 Rv3652 Rv3653: Rv3653 - PE-PGRS family-related protein PE_PGRS61, at 4,093,940 to 4,094,527 Rv3653 Rv3654c: Rv3654c - Conserved hypothetical protein, at 4,094,660 to 4,094,914 Rv3654c Rv3655c: Rv3655c - Conserved hypothetical protein, at 4,094,923 to 4,095,300 Rv3655c Rv3656c: Rv3656c - Conserved hypothetical protein, at 4,095,324 to 4,095,530 Rv3656c Rv3657c: Rv3657c - Possible conserved alanine rich membrane protein, at 4,095,540 to 4,096,115 Rv3657c Position (kb) 4094 4095Strain fitness (log2 ratio) -2 -1 0 1at 4093.792 kb on - strand, within Rv3652at 4093.814 kb on + strand, within Rv3652at 4093.814 kb on + strand, within Rv3652at 4093.815 kb on - strand, within Rv3652at 4094.562 kb on - strandat 4094.585 kb on - strandat 4094.613 kb on - strandat 4094.613 kb on - strandat 4094.650 kb on + strandat 4094.924 kb on + strandat 4094.924 kb on + strandat 4094.929 kb on - strandat 4095.164 kb on - strand, within Rv3655cat 4095.164 kb on - strand, within Rv3655cat 4095.210 kb on + strand, within Rv3655cat 4095.211 kb on - strand, within Rv3655cat 4095.445 kb on + strand, within Rv3656cat 4095.531 kb on - strandat 4095.583 kb on - strandat 4095.777 kb on + strand, within Rv3657c

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Isoniazid 0.025 ug/mL
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4,093,792 - Rv3652 0.51 -0.5
4,093,814 + Rv3652 0.58 -2.0
4,093,814 + Rv3652 0.58 -1.0
4,093,815 - Rv3652 0.58 -0.2
4,094,562 - -0.5
4,094,585 - -2.1
4,094,613 - +0.1
4,094,613 - +0.4
4,094,650 + -1.0
4,094,924 + +0.2
4,094,924 + -1.3
4,094,929 - -0.1
4,095,164 - Rv3655c 0.64 +1.3
4,095,164 - Rv3655c 0.64 -0.7
4,095,210 + Rv3655c 0.76 +0.3
4,095,211 - Rv3655c 0.76 -0.5
4,095,445 + Rv3656c 0.58 +1.6
4,095,531 - +0.0
4,095,583 - -0.6
4,095,777 + Rv3657c 0.41 -0.9

Or see this region's nucleotide sequence