Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv3578

Experiment: 7H9 with Isoniazid 0.025 ug/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv3577 and Rv3578 are separated by 13 nucleotidesRv3578 and Rv3579c are separated by 41 nucleotides Rv3577: Rv3577 - Conserved hypothetical protein, at 4,019,262 to 4,020,128 Rv3577 Rv3578: Rv3578 - Possible arsenical pump integral membrane protein ArsB2, at 4,020,142 to 4,021,383 Rv3578 Rv3579c: Rv3579c - Possible tRNA/rRNA methyltransferase, at 4,021,425 to 4,022,393 Rv3579c Position (kb) 4020 4021 4022Strain fitness (log2 ratio) -1 0 1 2 3at 4019.258 kb on + strandat 4019.863 kb on - strand, within Rv3577at 4019.901 kb on + strand, within Rv3577at 4019.902 kb on - strand, within Rv3577at 4019.902 kb on - strand, within Rv3577at 4019.902 kb on - strand, within Rv3577at 4020.032 kb on - strand, within Rv3577at 4020.032 kb on - strand, within Rv3577at 4020.136 kb on + strandat 4020.556 kb on + strand, within Rv3578at 4020.557 kb on - strand, within Rv3578at 4020.628 kb on + strand, within Rv3578at 4020.628 kb on + strand, within Rv3578at 4020.706 kb on + strand, within Rv3578at 4020.706 kb on + strand, within Rv3578at 4020.707 kb on - strand, within Rv3578at 4020.740 kb on + strand, within Rv3578at 4021.220 kb on - strand, within Rv3578

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Isoniazid 0.025 ug/mL
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4,019,258 + -1.4
4,019,863 - Rv3577 0.69 -0.4
4,019,901 + Rv3577 0.74 -1.5
4,019,902 - Rv3577 0.74 -0.1
4,019,902 - Rv3577 0.74 -0.8
4,019,902 - Rv3577 0.74 -0.8
4,020,032 - Rv3577 0.89 -0.6
4,020,032 - Rv3577 0.89 -0.7
4,020,136 + -0.3
4,020,556 + Rv3578 0.33 -0.5
4,020,557 - Rv3578 0.33 -0.1
4,020,628 + Rv3578 0.39 -0.0
4,020,628 + Rv3578 0.39 +3.1
4,020,706 + Rv3578 0.45 -0.1
4,020,706 + Rv3578 0.45 +0.3
4,020,707 - Rv3578 0.45 +1.4
4,020,740 + Rv3578 0.48 -0.9
4,021,220 - Rv3578 0.87 -1.0

Or see this region's nucleotide sequence