Experiment: 7H9 with Isoniazid 0.025 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv0519c and Rv0520 are separated by 180 nucleotides Rv0520 and Rv0521 overlap by 8 nucleotides Rv0521 and Rv0522 are separated by 134 nucleotides Rv0522 and Rv0523c overlap by 17 nucleotides Rv0523c and Rv0524 are separated by 113 nucleotides
Rv0519c: Rv0519c - Possible conserved membrane protein, at 611,172 to 612,074
Rv0519c
Rv0520: Rv0520 - Possible methyltransferase/methylase (fragment), at 612,255 to 612,605
Rv0520
Rv0521: Rv0521 - Possible methyltransferase/methylase (fragment), at 612,598 to 612,903
Rv0521
Rv0522: Rv0522 - Probable GABA permease GabP (4-amino butyrate transport carrier) (GAMA-aminobutyrate permease), at 613,038 to 614,342
Rv0522
Rv0523c: Rv0523c - Conserved protein, at 614,326 to 614,721
Rv0523c
Rv0524: Rv0524 - Probable glutamate-1-semialdehyde 2,1-aminomutase HemL (GSA) (glutamate-1-semialdehyde aminotransferase) (GSA-at), at 614,835 to 616,223
Rv0524
Position (kb) 613
614
615 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 612.193 kb on - strand at 612.254 kb on + strand at 612.255 kb on - strand at 612.314 kb on + strand, within Rv0520 at 612.314 kb on + strand, within Rv0520 at 612.315 kb on - strand, within Rv0520 at 612.400 kb on - strand, within Rv0520 at 612.555 kb on + strand, within Rv0520 at 612.597 kb on + strand at 612.598 kb on - strand at 612.598 kb on - strand at 612.674 kb on - strand, within Rv0521 at 612.674 kb on - strand, within Rv0521 at 612.759 kb on - strand, within Rv0521 at 612.846 kb on - strand, within Rv0521 at 612.956 kb on - strand at 612.988 kb on + strand at 612.988 kb on + strand at 612.989 kb on - strand at 612.993 kb on - strand at 613.047 kb on - strand at 613.128 kb on + strand at 613.219 kb on - strand, within Rv0522 at 613.269 kb on + strand, within Rv0522 at 613.328 kb on - strand, within Rv0522 at 613.627 kb on + strand, within Rv0522 at 613.785 kb on + strand, within Rv0522 at 613.811 kb on + strand, within Rv0522 at 613.884 kb on + strand, within Rv0522 at 613.884 kb on + strand, within Rv0522 at 613.884 kb on + strand, within Rv0522 at 613.884 kb on + strand, within Rv0522 at 613.885 kb on - strand, within Rv0522 at 613.885 kb on - strand, within Rv0522 at 613.885 kb on - strand, within Rv0522 at 613.885 kb on - strand, within Rv0522 at 613.885 kb on - strand, within Rv0522 at 614.156 kb on - strand, within Rv0522 at 614.295 kb on + strand at 614.296 kb on - strand at 614.296 kb on - strand at 614.348 kb on - strand at 614.402 kb on + strand, within Rv0523c at 614.403 kb on - strand, within Rv0523c at 614.684 kb on + strand at 614.685 kb on - strand at 614.685 kb on - strand at 614.713 kb on + strand at 614.827 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Isoniazid 0.025 ug/mL remove 612,193 - -2.9 612,254 + -1.6 612,255 - -1.0 612,314 + Rv0520 0.17 +1.2 612,314 + Rv0520 0.17 +0.8 612,315 - Rv0520 0.17 +0.3 612,400 - Rv0520 0.41 -1.1 612,555 + Rv0520 0.85 -1.5 612,597 + -2.9 612,598 - +0.7 612,598 - -1.7 612,674 - Rv0521 0.25 +0.7 612,674 - Rv0521 0.25 +0.2 612,759 - Rv0521 0.53 -0.4 612,846 - Rv0521 0.81 -0.3 612,956 - +0.1 612,988 + -0.9 612,988 + -0.6 612,989 - -0.0 612,993 - +0.2 613,047 - -0.8 613,128 + -0.3 613,219 - Rv0522 0.14 +0.6 613,269 + Rv0522 0.18 -0.6 613,328 - Rv0522 0.22 +1.7 613,627 + Rv0522 0.45 +2.3 613,785 + Rv0522 0.57 -1.0 613,811 + Rv0522 0.59 +0.5 613,884 + Rv0522 0.65 +2.3 613,884 + Rv0522 0.65 +0.7 613,884 + Rv0522 0.65 -0.4 613,884 + Rv0522 0.65 -0.4 613,885 - Rv0522 0.65 +0.0 613,885 - Rv0522 0.65 -0.8 613,885 - Rv0522 0.65 -0.4 613,885 - Rv0522 0.65 -0.7 613,885 - Rv0522 0.65 -0.1 614,156 - Rv0522 0.86 +0.5 614,295 + -0.3 614,296 - -0.8 614,296 - -1.1 614,348 - -1.3 614,402 + Rv0523c 0.19 -1.6 614,403 - Rv0523c 0.19 -0.1 614,684 + +0.6 614,685 - -0.8 614,685 - +3.8 614,713 + -0.9 614,827 + +1.1
Or see this region's nucleotide sequence