Experiment: 7H9 with Isoniazid 0.025 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv0518 and Rv0519c are separated by 288 nucleotides Rv0519c and Rv0520 are separated by 180 nucleotides Rv0520 and Rv0521 overlap by 8 nucleotides Rv0521 and Rv0522 are separated by 134 nucleotides
Rv0518: Rv0518 - Possible exported protein, at 610,188 to 610,883
Rv0518
Rv0519c: Rv0519c - Possible conserved membrane protein, at 611,172 to 612,074
Rv0519c
Rv0520: Rv0520 - Possible methyltransferase/methylase (fragment), at 612,255 to 612,605
Rv0520
Rv0521: Rv0521 - Possible methyltransferase/methylase (fragment), at 612,598 to 612,903
Rv0521
Rv0522: Rv0522 - Probable GABA permease GabP (4-amino butyrate transport carrier) (GAMA-aminobutyrate permease), at 613,038 to 614,342
Rv0522
Position (kb) 611
612
613 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 610.202 kb on + strand at 610.203 kb on - strand at 610.206 kb on + strand at 610.206 kb on + strand at 610.206 kb on + strand at 610.206 kb on + strand at 610.206 kb on + strand at 610.206 kb on + strand at 610.207 kb on - strand at 610.207 kb on - strand at 610.207 kb on - strand at 610.207 kb on - strand at 610.207 kb on - strand at 610.207 kb on - strand at 610.207 kb on - strand at 610.207 kb on - strand at 610.207 kb on - strand at 610.326 kb on + strand, within Rv0518 at 610.327 kb on - strand, within Rv0518 at 610.328 kb on + strand, within Rv0518 at 610.328 kb on + strand, within Rv0518 at 610.668 kb on - strand, within Rv0518 at 610.678 kb on + strand, within Rv0518 at 610.679 kb on - strand, within Rv0518 at 610.833 kb on + strand at 610.834 kb on - strand at 610.936 kb on + strand at 610.937 kb on - strand at 610.937 kb on - strand at 610.957 kb on + strand at 611.081 kb on - strand at 611.173 kb on + strand at 611.174 kb on - strand at 611.174 kb on - strand at 611.300 kb on - strand, within Rv0519c at 611.380 kb on + strand, within Rv0519c at 611.381 kb on - strand, within Rv0519c at 611.507 kb on - strand, within Rv0519c at 611.553 kb on - strand, within Rv0519c at 611.632 kb on + strand, within Rv0519c at 611.632 kb on + strand, within Rv0519c at 611.633 kb on - strand, within Rv0519c at 612.193 kb on - strand at 612.254 kb on + strand at 612.255 kb on - strand at 612.314 kb on + strand, within Rv0520 at 612.314 kb on + strand, within Rv0520 at 612.315 kb on - strand, within Rv0520 at 612.400 kb on - strand, within Rv0520 at 612.555 kb on + strand, within Rv0520 at 612.597 kb on + strand at 612.598 kb on - strand at 612.598 kb on - strand at 612.674 kb on - strand, within Rv0521 at 612.674 kb on - strand, within Rv0521 at 612.759 kb on - strand, within Rv0521 at 612.846 kb on - strand, within Rv0521 at 612.956 kb on - strand at 612.988 kb on + strand at 612.988 kb on + strand at 612.989 kb on - strand at 612.993 kb on - strand at 613.047 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Isoniazid 0.025 ug/mL remove 610,202 + +1.5 610,203 - +0.2 610,206 + +1.6 610,206 + -0.6 610,206 + +0.4 610,206 + -0.7 610,206 + -0.4 610,206 + +3.2 610,207 - -1.7 610,207 - -1.7 610,207 - +0.2 610,207 - +0.5 610,207 - -0.6 610,207 - -0.9 610,207 - -0.2 610,207 - +0.4 610,207 - +0.5 610,326 + Rv0518 0.20 -0.4 610,327 - Rv0518 0.20 +0.5 610,328 + Rv0518 0.20 -0.3 610,328 + Rv0518 0.20 +0.6 610,668 - Rv0518 0.69 +0.8 610,678 + Rv0518 0.70 +0.5 610,679 - Rv0518 0.71 -0.2 610,833 + +0.2 610,834 - -1.3 610,936 + -1.2 610,937 - -0.1 610,937 - -0.2 610,957 + -0.7 611,081 - +0.5 611,173 + -0.2 611,174 - -2.6 611,174 - -0.2 611,300 - Rv0519c 0.14 +1.0 611,380 + Rv0519c 0.23 +2.2 611,381 - Rv0519c 0.23 +0.4 611,507 - Rv0519c 0.37 +0.0 611,553 - Rv0519c 0.42 +0.2 611,632 + Rv0519c 0.51 -0.2 611,632 + Rv0519c 0.51 -0.8 611,633 - Rv0519c 0.51 +1.8 612,193 - -2.9 612,254 + -1.6 612,255 - -1.0 612,314 + Rv0520 0.17 +1.2 612,314 + Rv0520 0.17 +0.8 612,315 - Rv0520 0.17 +0.3 612,400 - Rv0520 0.41 -1.1 612,555 + Rv0520 0.85 -1.5 612,597 + -2.9 612,598 - +0.7 612,598 - -1.7 612,674 - Rv0521 0.25 +0.7 612,674 - Rv0521 0.25 +0.2 612,759 - Rv0521 0.53 -0.4 612,846 - Rv0521 0.81 -0.3 612,956 - +0.1 612,988 + -0.9 612,988 + -0.6 612,989 - -0.0 612,993 - +0.2 613,047 - -0.8
Or see this region's nucleotide sequence