Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv0125

Experiment: 7H9 with Isoniazid 0.025 ug/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv0124 and Rv0125 are separated by 151 nucleotidesRv0125 and Rv0126 are separated by 108 nucleotides Rv0124: Rv0124 - PE-PGRS family protein PE_PGRS2, at 149,533 to 150,996 Rv0124 Rv0125: Rv0125 - Probable serine protease PepA (serine proteinase) (MTB32A), at 151,148 to 152,215 Rv0125 Rv0126: Rv0126 - Trehalose synthase TreS, at 152,324 to 154,129 Rv0126 Position (kb) 151 152 153Strain fitness (log2 ratio) -1 0 1 2at 150.286 kb on + strand, within Rv0124at 150.393 kb on + strand, within Rv0124at 150.393 kb on + strand, within Rv0124at 150.433 kb on - strand, within Rv0124at 150.872 kb on + strandat 150.872 kb on + strandat 150.873 kb on - strandat 151.064 kb on - strandat 151.073 kb on + strandat 151.100 kb on - strandat 151.120 kb on + strandat 151.124 kb on + strandat 151.125 kb on - strandat 151.125 kb on - strandat 151.361 kb on + strand, within Rv0125at 151.361 kb on + strand, within Rv0125at 151.361 kb on + strand, within Rv0125at 151.362 kb on - strand, within Rv0125at 151.487 kb on + strand, within Rv0125at 151.487 kb on + strand, within Rv0125at 151.508 kb on + strand, within Rv0125at 151.795 kb on - strand, within Rv0125at 151.832 kb on - strand, within Rv0125at 151.832 kb on - strand, within Rv0125at 152.114 kb on - strandat 152.642 kb on + strand, within Rv0126

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Isoniazid 0.025 ug/mL
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150,286 + Rv0124 0.51 +0.5
150,393 + Rv0124 0.59 -0.1
150,393 + Rv0124 0.59 -1.6
150,433 - Rv0124 0.61 +0.1
150,872 + +1.1
150,872 + -0.2
150,873 - +2.0
151,064 - -0.8
151,073 + -0.8
151,100 - -0.4
151,120 + +0.4
151,124 + -0.4
151,125 - +2.3
151,125 - +0.6
151,361 + Rv0125 0.20 -0.5
151,361 + Rv0125 0.20 +0.2
151,361 + Rv0125 0.20 +1.1
151,362 - Rv0125 0.20 -0.7
151,487 + Rv0125 0.32 -0.9
151,487 + Rv0125 0.32 -0.1
151,508 + Rv0125 0.34 +0.1
151,795 - Rv0125 0.61 -1.1
151,832 - Rv0125 0.64 +2.3
151,832 - Rv0125 0.64 +0.2
152,114 - -1.1
152,642 + Rv0126 0.18 +2.3

Or see this region's nucleotide sequence