Experiment: 7H9 with Isoniazid 0.025 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv0008c and Rv0009 are separated by 156 nucleotides Rv0009 and Rv0010c are separated by 116 nucleotides Rv0010c and Rv0011c are separated by 155 nucleotides Rv0011c and Rv0012 are separated by 93 nucleotides
Rv0008c: Rv0008c - Possible membrane protein, at 11,874 to 12,311
Rv0008c
Rv0009: Rv0009 - Probable iron-regulated peptidyl-prolyl cis-trans isomerase A PpiA (PPIase A) (rotamase A), at 12,468 to 13,016
Rv0009
Rv0010c: Rv0010c - Probable conserved membrane protein, at 13,133 to 13,558
Rv0010c
Rv0011c: Rv0011c - Probable conserved transmembrane protein, at 13,714 to 13,995
Rv0011c
Rv0012: Rv0012 - Probable conserved membrane protein, at 14,089 to 14,877
Rv0012
Position (kb) 13
14 Strain fitness (log2 ratio) -2
-1
0
1 at 12.353 kb on - strand at 12.492 kb on - strand at 12.512 kb on + strand at 12.621 kb on + strand, within Rv0009 at 13.025 kb on + strand at 13.025 kb on + strand at 13.156 kb on - strand at 13.309 kb on + strand, within Rv0010c at 13.309 kb on + strand, within Rv0010c at 13.323 kb on - strand, within Rv0010c at 13.323 kb on - strand, within Rv0010c at 13.325 kb on + strand, within Rv0010c at 13.326 kb on - strand, within Rv0010c at 13.326 kb on - strand, within Rv0010c at 13.369 kb on + strand, within Rv0010c at 13.568 kb on - strand at 14.059 kb on + strand at 14.059 kb on + strand at 14.089 kb on - strand at 14.097 kb on - strand at 14.097 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Isoniazid 0.025 ug/mL remove 12,353 - -1.5 12,492 - -0.7 12,512 + -0.9 12,621 + Rv0009 0.28 +0.1 13,025 + +1.3 13,025 + -1.4 13,156 - -0.7 13,309 + Rv0010c 0.41 -1.9 13,309 + Rv0010c 0.41 +1.3 13,323 - Rv0010c 0.45 -0.3 13,323 - Rv0010c 0.45 -0.9 13,325 + Rv0010c 0.45 +1.3 13,326 - Rv0010c 0.45 -0.5 13,326 - Rv0010c 0.45 -1.0 13,369 + Rv0010c 0.55 -0.6 13,568 - -1.0 14,059 + +1.3 14,059 + -1.6 14,089 - +0.0 14,097 - +0.2 14,097 - -1.0
Or see this region's nucleotide sequence