Experiment: 7H9 with Isoniazid 0.025 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv2679 and Rv2680 are separated by 159 nucleotides Rv2680 and Rv2681 are separated by 1 nucleotides Rv2681 and Rv2682c overlap by 4 nucleotides
Rv2679: Rv2679 - Probable enoyl-CoA hydratase EchA15 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase), at 2,995,115 to 2,995,945
Rv2679
Rv2680: Rv2680 - Conserved protein, at 2,996,105 to 2,996,737
Rv2680
Rv2681: Rv2681 - Conserved hypothetical alanine rich protein, at 2,996,739 to 2,998,055
Rv2681
Rv2682c: Rv2682c - Probable 1-deoxy-D-xylulose 5-phosphate synthase Dxs1 (1-deoxyxylulose-5-phosphate synthase) (DXP synthase) (DXPS), at 2,998,052 to 2,999,968
Rv2682c
Position (kb)
2996
2997
2998
2999 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 2995.797 kb on - strand, within Rv2679 at 2995.989 kb on + strand at 2996.277 kb on - strand, within Rv2680 at 2996.367 kb on - strand, within Rv2680 at 2996.402 kb on - strand, within Rv2680 at 2996.875 kb on + strand, within Rv2681 at 2996.987 kb on - strand, within Rv2681 at 2997.116 kb on + strand, within Rv2681 at 2997.133 kb on + strand, within Rv2681 at 2997.135 kb on + strand, within Rv2681 at 2997.136 kb on - strand, within Rv2681 at 2997.136 kb on - strand, within Rv2681 at 2997.206 kb on - strand, within Rv2681 at 2997.283 kb on - strand, within Rv2681 at 2997.576 kb on - strand, within Rv2681 at 2997.576 kb on - strand, within Rv2681 at 2997.942 kb on + strand at 2997.943 kb on - strand at 2997.982 kb on + strand at 2997.982 kb on + strand at 2997.982 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Isoniazid 0.025 ug/mL remove 2,995,797 - Rv2679 0.82 +0.8 2,995,989 + -0.7 2,996,277 - Rv2680 0.27 -1.2 2,996,367 - Rv2680 0.41 +0.2 2,996,402 - Rv2680 0.47 -1.0 2,996,875 + Rv2681 0.10 +4.1 2,996,987 - Rv2681 0.19 +2.2 2,997,116 + Rv2681 0.29 +2.9 2,997,133 + Rv2681 0.30 +3.8 2,997,135 + Rv2681 0.30 +1.4 2,997,136 - Rv2681 0.30 +0.8 2,997,136 - Rv2681 0.30 +1.8 2,997,206 - Rv2681 0.35 +1.3 2,997,283 - Rv2681 0.41 +0.4 2,997,576 - Rv2681 0.64 +0.6 2,997,576 - Rv2681 0.64 +0.3 2,997,942 + +0.0 2,997,943 - -1.6 2,997,982 + +1.1 2,997,982 + -0.0 2,997,982 + +0.9
Or see this region's nucleotide sequence