Experiment: 7H9 with Isoniazid 0.025 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv2000 and Rv2001 are separated by 9 nucleotides Rv2001 and Rv2002 are separated by 75 nucleotides Rv2002 and Rv2003c are separated by 120 nucleotides
Rv2000: Rv2000 - Unknown protein, at 2,245,209 to 2,246,822
Rv2000
Rv2001: Rv2001 - Conserved hypothetical protein, at 2,246,832 to 2,247,584
Rv2001
Rv2002: Rv2002 - Possible 20-beta-hydroxysteroid dehydrogenase FabG3 (cortisone reductase) ((R)-20-hydroxysteroid dehydrogenase), at 2,247,660 to 2,248,442
Rv2002
Rv2003c: Rv2003c - Conserved hypothetical protein, at 2,248,563 to 2,249,420
Rv2003c
Position (kb)
2247
2248
2249 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2246.776 kb on + strand at 2246.893 kb on - strand at 2246.893 kb on - strand at 2247.409 kb on + strand, within Rv2001 at 2247.410 kb on - strand, within Rv2001 at 2247.580 kb on + strand at 2247.580 kb on + strand at 2247.713 kb on + strand at 2248.376 kb on - strand at 2248.440 kb on + strand at 2248.565 kb on - strand at 2248.593 kb on - strand at 2248.771 kb on + strand, within Rv2003c at 2248.823 kb on - strand, within Rv2003c at 2249.326 kb on - strand, within Rv2003c
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Isoniazid 0.025 ug/mL remove 2,246,776 + -1.8 2,246,893 - -0.5 2,246,893 - -0.2 2,247,409 + Rv2001 0.77 -2.1 2,247,410 - Rv2001 0.77 +0.8 2,247,580 + +2.8 2,247,580 + -0.8 2,247,713 + -0.2 2,248,376 - +0.2 2,248,440 + -1.7 2,248,565 - +0.1 2,248,593 - -0.6 2,248,771 + Rv2003c 0.24 -1.0 2,248,823 - Rv2003c 0.30 +1.8 2,249,326 - Rv2003c 0.89 -0.2
Or see this region's nucleotide sequence