Experiment: 7H9 with Isoniazid 0.025 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv1850 and Rv1851 overlap by 1 nucleotides Rv1851 and Rv1852 are separated by 10 nucleotides Rv1852 and Rv1853 are separated by 7 nucleotides Rv1853 and Rv1854c are separated by 2 nucleotides
Rv1850: Rv1850 - Urease alpha subunit UreC (urea amidohydrolase), at 2,097,961 to 2,099,694
Rv1850
Rv1851: Rv1851 - Urease accessory protein UreF, at 2,099,694 to 2,100,329
Rv1851
Rv1852: Rv1852 - Urease accessory protein UreG, at 2,100,340 to 2,101,014
Rv1852
Rv1853: Rv1853 - Probable urease accessory protein UreD, at 2,101,022 to 2,101,648
Rv1853
Rv1854c: Rv1854c - Probable NADH dehydrogenase Ndh, at 2,101,651 to 2,103,042
Rv1854c
Position (kb)
2100
2101
2102 Strain fitness (log2 ratio)
-1
0
1 at 2099.523 kb on - strand at 2099.663 kb on + strand at 2099.668 kb on - strand at 2099.680 kb on + strand at 2099.681 kb on - strand at 2100.120 kb on + strand, within Rv1851 at 2100.169 kb on + strand, within Rv1851 at 2100.526 kb on + strand, within Rv1852 at 2101.013 kb on - strand at 2101.526 kb on - strand, within Rv1853 at 2101.619 kb on - strand at 2101.652 kb on + strand at 2101.665 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Isoniazid 0.025 ug/mL remove 2,099,523 - +0.0 2,099,663 + -0.1 2,099,668 - +1.0 2,099,680 + +0.4 2,099,681 - -0.5 2,100,120 + Rv1851 0.67 +1.0 2,100,169 + Rv1851 0.75 +1.5 2,100,526 + Rv1852 0.28 -0.0 2,101,013 - -0.9 2,101,526 - Rv1853 0.80 +0.4 2,101,619 - -1.4 2,101,652 + -0.5 2,101,665 + -0.9
Or see this region's nucleotide sequence