Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv1082

Experiment: 7H9 with Isoniazid 0.025 ug/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv1080c and Rv1081c are separated by 185 nucleotidesRv1081c and Rv1082 are separated by 101 nucleotidesRv1082 and Rv1083 overlap by 4 nucleotidesRv1083 and Rv1084 overlap by 14 nucleotides Rv1080c: Rv1080c - Probable transcription elongation factor GreA (transcript cleavage factor GreA), at 1,205,304 to 1,205,798 Rv1080c Rv1081c: Rv1081c - Probable conserved membrane protein, at 1,205,984 to 1,206,418 Rv1081c Rv1082: Rv1082 - Mycothiol conjugate amidase Mca (mycothiol S-conjugate amidase), at 1,206,520 to 1,207,386 Rv1082 Rv1083: Rv1083 - Conserved hypothetical protein, at 1,207,383 to 1,207,649 Rv1083 Rv1084: Rv1084 - Conserved protein, at 1,207,636 to 1,209,657 Rv1084 Position (kb) 1206 1207 1208Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1205.831 kb on + strandat 1205.927 kb on - strandat 1205.938 kb on - strandat 1205.938 kb on - strandat 1205.974 kb on - strandat 1206.466 kb on - strandat 1206.468 kb on - strandat 1206.478 kb on - strandat 1206.478 kb on - strandat 1206.478 kb on - strandat 1206.479 kb on + strandat 1206.789 kb on - strand, within Rv1082at 1206.792 kb on + strand, within Rv1082at 1206.793 kb on - strand, within Rv1082at 1206.803 kb on + strand, within Rv1082at 1206.910 kb on + strand, within Rv1082at 1207.042 kb on + strand, within Rv1082at 1207.042 kb on + strand, within Rv1082at 1207.045 kb on + strand, within Rv1082at 1207.046 kb on - strand, within Rv1082at 1207.335 kb on + strandat 1207.648 kb on - strandat 1207.691 kb on - strandat 1207.710 kb on + strandat 1207.927 kb on + strand, within Rv1084at 1207.928 kb on - strand, within Rv1084at 1207.928 kb on - strand, within Rv1084at 1208.021 kb on - strand, within Rv1084at 1208.282 kb on + strand, within Rv1084at 1208.294 kb on - strand, within Rv1084at 1208.369 kb on - strand, within Rv1084at 1208.369 kb on - strand, within Rv1084

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Isoniazid 0.025 ug/mL
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1,205,831 + -1.0
1,205,927 - -0.0
1,205,938 - +0.1
1,205,938 - -0.2
1,205,974 - -1.0
1,206,466 - -0.1
1,206,468 - +0.2
1,206,478 - +0.2
1,206,478 - -1.0
1,206,478 - -0.2
1,206,479 + -1.0
1,206,789 - Rv1082 0.31 -2.8
1,206,792 + Rv1082 0.31 +0.4
1,206,793 - Rv1082 0.31 -2.0
1,206,803 + Rv1082 0.33 -0.7
1,206,910 + Rv1082 0.45 -0.7
1,207,042 + Rv1082 0.60 -0.4
1,207,042 + Rv1082 0.60 -2.7
1,207,045 + Rv1082 0.61 +0.2
1,207,046 - Rv1082 0.61 +1.8
1,207,335 + +1.0
1,207,648 - +0.2
1,207,691 - -0.5
1,207,710 + +1.0
1,207,927 + Rv1084 0.14 -0.3
1,207,928 - Rv1084 0.14 +0.3
1,207,928 - Rv1084 0.14 -0.4
1,208,021 - Rv1084 0.19 -0.2
1,208,282 + Rv1084 0.32 -0.3
1,208,294 - Rv1084 0.33 -0.8
1,208,369 - Rv1084 0.36 -0.9
1,208,369 - Rv1084 0.36 -0.5

Or see this region's nucleotide sequence