Experiment: 7H9 with Isoniazid 0.025 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv0221 and Rv0222 are separated by 30 nucleotides Rv0222 and Rv0223c are separated by 5 nucleotides
Rv0221: Rv0221 - Possible triacylglycerol synthase (diacylglycerol acyltransferase), at 264,067 to 265,476
Rv0221
Rv0222: Rv0222 - Probable enoyl-CoA hydratase EchA1 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase), at 265,507 to 266,295
Rv0222
Rv0223c: Rv0223c - Probable aldehyde dehydrogenase, at 266,301 to 267,764
Rv0223c
Position (kb) 265
266
267 Strain fitness (log2 ratio) -1
0
1
2 at 264.562 kb on + strand, within Rv0221 at 264.583 kb on - strand, within Rv0221 at 264.659 kb on - strand, within Rv0221 at 264.659 kb on - strand, within Rv0221 at 264.730 kb on - strand, within Rv0221 at 264.898 kb on + strand, within Rv0221 at 264.899 kb on - strand, within Rv0221 at 265.261 kb on + strand, within Rv0221 at 265.299 kb on + strand, within Rv0221 at 265.315 kb on + strand, within Rv0221 at 265.316 kb on - strand, within Rv0221 at 265.332 kb on + strand, within Rv0221 at 265.507 kb on - strand at 265.832 kb on - strand, within Rv0222 at 265.976 kb on - strand, within Rv0222 at 266.016 kb on - strand, within Rv0222 at 266.016 kb on - strand, within Rv0222 at 266.152 kb on - strand, within Rv0222 at 266.184 kb on + strand, within Rv0222 at 266.449 kb on + strand, within Rv0223c at 266.527 kb on + strand, within Rv0223c at 266.573 kb on - strand, within Rv0223c at 266.660 kb on + strand, within Rv0223c at 266.740 kb on + strand, within Rv0223c at 266.740 kb on + strand, within Rv0223c at 267.006 kb on + strand, within Rv0223c at 267.080 kb on + strand, within Rv0223c
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Isoniazid 0.025 ug/mL remove 264,562 + Rv0221 0.35 -0.5 264,583 - Rv0221 0.37 +0.8 264,659 - Rv0221 0.42 +0.3 264,659 - Rv0221 0.42 +1.8 264,730 - Rv0221 0.47 -0.0 264,898 + Rv0221 0.59 -0.3 264,899 - Rv0221 0.59 +1.3 265,261 + Rv0221 0.85 -0.0 265,299 + Rv0221 0.87 -0.7 265,315 + Rv0221 0.89 -0.5 265,316 - Rv0221 0.89 -0.3 265,332 + Rv0221 0.90 +1.2 265,507 - -0.7 265,832 - Rv0222 0.41 +1.0 265,976 - Rv0222 0.59 -0.9 266,016 - Rv0222 0.65 -0.1 266,016 - Rv0222 0.65 +0.5 266,152 - Rv0222 0.82 -0.3 266,184 + Rv0222 0.86 +0.5 266,449 + Rv0223c 0.10 +0.8 266,527 + Rv0223c 0.15 -0.6 266,573 - Rv0223c 0.19 -1.0 266,660 + Rv0223c 0.25 -0.6 266,740 + Rv0223c 0.30 +0.6 266,740 + Rv0223c 0.30 +1.5 267,006 + Rv0223c 0.48 +1.0 267,080 + Rv0223c 0.53 +0.6
Or see this region's nucleotide sequence