Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv3365c

Experiment: 7H9 with Levofloxacin 0.3 ug/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv3362c and Rv3363c overlap by 20 nucleotidesRv3363c and Rv3364c overlap by 23 nucleotidesRv3364c and Rv3365c overlap by 4 nucleotidesRv3365c and Rv3366 are separated by 235 nucleotides Rv3362c: Rv3362c - Probable ATP/GTP-binding protein, at 3,773,574 to 3,774,155 Rv3362c Rv3363c: Rv3363c - Conserved hypothetical protein, at 3,774,136 to 3,774,504 Rv3363c Rv3364c: Rv3364c - Conserved protein, at 3,774,482 to 3,774,874 Rv3364c Rv3365c: Rv3365c - Conserved protein, at 3,774,871 to 3,777,501 Rv3365c Rv3366: Rv3366 - Probable tRNA/rRNA methylase SpoU (tRNA/rRNA methyltransferase), at 3,777,737 to 3,778,201 Rv3366 Position (kb) 3774 3775 3776 3777 3778Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2 3 4 5at 3774.436 kb on - strand, within Rv3363cat 3774.436 kb on - strand, within Rv3363cat 3774.455 kb on + strand, within Rv3363cat 3774.456 kb on - strand, within Rv3363cat 3774.484 kb on - strandat 3774.746 kb on + strand, within Rv3364cat 3774.746 kb on + strand, within Rv3364cat 3775.088 kb on - strandat 3775.122 kb on - strandat 3775.368 kb on + strand, within Rv3365cat 3775.368 kb on + strand, within Rv3365cat 3775.368 kb on + strand, within Rv3365cat 3775.369 kb on - strand, within Rv3365cat 3775.558 kb on + strand, within Rv3365cat 3775.616 kb on + strand, within Rv3365cat 3775.617 kb on - strand, within Rv3365cat 3775.708 kb on + strand, within Rv3365cat 3775.955 kb on - strand, within Rv3365cat 3776.354 kb on + strand, within Rv3365cat 3776.354 kb on + strand, within Rv3365cat 3776.354 kb on + strand, within Rv3365cat 3776.354 kb on + strand, within Rv3365cat 3776.355 kb on - strand, within Rv3365cat 3776.355 kb on - strand, within Rv3365cat 3776.355 kb on - strand, within Rv3365cat 3776.355 kb on - strand, within Rv3365cat 3776.456 kb on - strand, within Rv3365cat 3776.999 kb on + strand, within Rv3365cat 3777.140 kb on + strand, within Rv3365cat 3777.141 kb on - strand, within Rv3365cat 3777.141 kb on - strand, within Rv3365cat 3777.227 kb on + strand, within Rv3365cat 3777.227 kb on + strand, within Rv3365cat 3777.227 kb on + strand, within Rv3365cat 3777.227 kb on + strand, within Rv3365cat 3777.227 kb on + strand, within Rv3365cat 3777.228 kb on - strand, within Rv3365cat 3777.228 kb on - strand, within Rv3365cat 3777.228 kb on - strand, within Rv3365cat 3777.450 kb on + strandat 3777.563 kb on + strandat 3777.563 kb on + strandat 3777.618 kb on + strandat 3777.642 kb on - strandat 3777.642 kb on - strandat 3777.756 kb on + strandat 3777.756 kb on + strandat 3777.757 kb on - strandat 3777.888 kb on - strand, within Rv3366at 3778.075 kb on + strand, within Rv3366at 3778.075 kb on + strand, within Rv3366at 3778.232 kb on + strandat 3778.233 kb on - strandat 3778.373 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Levofloxacin 0.3 ug/mL
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3,774,436 - Rv3363c 0.81 +1.9
3,774,436 - Rv3363c 0.81 +0.4
3,774,455 + Rv3363c 0.86 -1.3
3,774,456 - Rv3363c 0.87 +2.6
3,774,484 - +0.4
3,774,746 + Rv3364c 0.67 -0.6
3,774,746 + Rv3364c 0.67 -5.0
3,775,088 - +1.8
3,775,122 - -0.8
3,775,368 + Rv3365c 0.19 -3.0
3,775,368 + Rv3365c 0.19 -2.4
3,775,368 + Rv3365c 0.19 +0.0
3,775,369 - Rv3365c 0.19 -2.1
3,775,558 + Rv3365c 0.26 -1.5
3,775,616 + Rv3365c 0.28 -1.8
3,775,617 - Rv3365c 0.28 -1.2
3,775,708 + Rv3365c 0.32 +0.0
3,775,955 - Rv3365c 0.41 +0.5
3,776,354 + Rv3365c 0.56 -1.2
3,776,354 + Rv3365c 0.56 +0.4
3,776,354 + Rv3365c 0.56 -2.5
3,776,354 + Rv3365c 0.56 -2.4
3,776,355 - Rv3365c 0.56 +0.8
3,776,355 - Rv3365c 0.56 -1.8
3,776,355 - Rv3365c 0.56 +1.0
3,776,355 - Rv3365c 0.56 +4.8
3,776,456 - Rv3365c 0.60 +0.2
3,776,999 + Rv3365c 0.81 -1.6
3,777,140 + Rv3365c 0.86 -2.2
3,777,141 - Rv3365c 0.86 +2.2
3,777,141 - Rv3365c 0.86 -2.0
3,777,227 + Rv3365c 0.90 -2.2
3,777,227 + Rv3365c 0.90 -3.9
3,777,227 + Rv3365c 0.90 -1.6
3,777,227 + Rv3365c 0.90 -2.7
3,777,227 + Rv3365c 0.90 -1.6
3,777,228 - Rv3365c 0.90 -0.6
3,777,228 - Rv3365c 0.90 +0.7
3,777,228 - Rv3365c 0.90 +0.0
3,777,450 + -1.5
3,777,563 + -1.4
3,777,563 + -1.5
3,777,618 + -0.0
3,777,642 - -0.0
3,777,642 - +1.8
3,777,756 + -0.0
3,777,756 + -0.5
3,777,757 - +2.8
3,777,888 - Rv3366 0.32 +1.8
3,778,075 + Rv3366 0.73 -1.9
3,778,075 + Rv3366 0.73 -1.5
3,778,232 + -0.4
3,778,233 - +0.9
3,778,373 + -1.0

Or see this region's nucleotide sequence