Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv3282

Experiment: 7H9 with Levofloxacin 0.3 ug/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv3280 and Rv3281 overlap by 20 nucleotidesRv3281 and Rv3282 overlap by 4 nucleotidesRv3282 and Rv3283 are separated by 40 nucleotidesRv3283 and Rv3284 overlap by 4 nucleotides Rv3280: Rv3280 - Probable propionyl-CoA carboxylase beta chain 5 AccD5 (pccase) (propanoyl-CoA:carbon dioxide ligase), at 3,662,062 to 3,663,708 Rv3280 Rv3281: Rv3281 - Probable bifunctional protein acetyl-/propionyl-coenzyme A carboxylase (epsilon chain) AccE5, at 3,663,689 to 3,664,222 Rv3281 Rv3282: Rv3282 - Conserved hypothetical protein, at 3,664,219 to 3,664,887 Rv3282 Rv3283: Rv3283 - Probable thiosulfate sulfurtransferase SseA (rhodanese) (thiosulfate cyanide transsulfurase) (thiosulfate thiotransferase), at 3,664,928 to 3,665,821 Rv3283 Rv3284: Rv3284 - Conserved hypothetical protein, at 3,665,818 to 3,666,249 Rv3284 Position (kb) 3664 3665Strain fitness (log2 ratio) -2 -1 0 1at 3664.564 kb on + strand, within Rv3282at 3664.678 kb on + strand, within Rv3282at 3664.886 kb on - strandat 3665.142 kb on - strand, within Rv3283at 3665.214 kb on - strand, within Rv3283at 3665.495 kb on - strand, within Rv3283at 3665.495 kb on - strand, within Rv3283at 3665.504 kb on + strand, within Rv3283at 3665.658 kb on - strand, within Rv3283at 3665.727 kb on + strand, within Rv3283at 3665.728 kb on - strand, within Rv3283at 3665.735 kb on + strandat 3665.736 kb on - strandat 3665.817 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Levofloxacin 0.3 ug/mL
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3,664,564 + Rv3282 0.52 -1.1
3,664,678 + Rv3282 0.69 -2.6
3,664,886 - -1.4
3,665,142 - Rv3283 0.24 -0.2
3,665,214 - Rv3283 0.32 +0.1
3,665,495 - Rv3283 0.63 +0.1
3,665,495 - Rv3283 0.63 +0.9
3,665,504 + Rv3283 0.64 -2.1
3,665,658 - Rv3283 0.82 -0.9
3,665,727 + Rv3283 0.89 -1.4
3,665,728 - Rv3283 0.89 +1.1
3,665,735 + +0.3
3,665,736 - +0.1
3,665,817 + -0.7

Or see this region's nucleotide sequence