Experiment: 7H9 with Levofloxacin 0.3 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv3280 and Rv3281 overlap by 20 nucleotides Rv3281 and Rv3282 overlap by 4 nucleotides Rv3282 and Rv3283 are separated by 40 nucleotides Rv3283 and Rv3284 overlap by 4 nucleotides
Rv3280: Rv3280 - Probable propionyl-CoA carboxylase beta chain 5 AccD5 (pccase) (propanoyl-CoA:carbon dioxide ligase), at 3,662,062 to 3,663,708
Rv3280
Rv3281: Rv3281 - Probable bifunctional protein acetyl-/propionyl-coenzyme A carboxylase (epsilon chain) AccE5, at 3,663,689 to 3,664,222
Rv3281
Rv3282: Rv3282 - Conserved hypothetical protein, at 3,664,219 to 3,664,887
Rv3282
Rv3283: Rv3283 - Probable thiosulfate sulfurtransferase SseA (rhodanese) (thiosulfate cyanide transsulfurase) (thiosulfate thiotransferase), at 3,664,928 to 3,665,821
Rv3283
Rv3284: Rv3284 - Conserved hypothetical protein, at 3,665,818 to 3,666,249
Rv3284
Position (kb)
3664
3665 Strain fitness (log2 ratio)
-2
-1
0
1 at 3664.564 kb on + strand, within Rv3282 at 3664.678 kb on + strand, within Rv3282 at 3664.886 kb on - strand at 3665.142 kb on - strand, within Rv3283 at 3665.214 kb on - strand, within Rv3283 at 3665.495 kb on - strand, within Rv3283 at 3665.495 kb on - strand, within Rv3283 at 3665.504 kb on + strand, within Rv3283 at 3665.658 kb on - strand, within Rv3283 at 3665.727 kb on + strand, within Rv3283 at 3665.728 kb on - strand, within Rv3283 at 3665.735 kb on + strand at 3665.736 kb on - strand at 3665.817 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Levofloxacin 0.3 ug/mL remove 3,664,564 + Rv3282 0.52 -1.1 3,664,678 + Rv3282 0.69 -2.6 3,664,886 - -1.4 3,665,142 - Rv3283 0.24 -0.2 3,665,214 - Rv3283 0.32 +0.1 3,665,495 - Rv3283 0.63 +0.1 3,665,495 - Rv3283 0.63 +0.9 3,665,504 + Rv3283 0.64 -2.1 3,665,658 - Rv3283 0.82 -0.9 3,665,727 + Rv3283 0.89 -1.4 3,665,728 - Rv3283 0.89 +1.1 3,665,735 + +0.3 3,665,736 - +0.1 3,665,817 + -0.7
Or see this region's nucleotide sequence