Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv3278c

Experiment: 7H9 with Levofloxacin 0.3 ug/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv3276c and Rv3277 overlap by 47 nucleotidesRv3277 and Rv3278c overlap by 46 nucleotidesRv3278c and Rv3279c are separated by 42 nucleotidesRv3279c and Rv3280 are separated by 49 nucleotides Rv3276c: Rv3276c - Probable phosphoribosylaminoimidazole carboxylase ATPase subunit PurK (air carboxylase) (AIRC), at 3,658,635 to 3,659,924 Rv3276c Rv3277: Rv3277 - Probable conserved transmembrane protein, at 3,659,878 to 3,660,696 Rv3277 Rv3278c: Rv3278c - Probable conserved transmembrane protein, at 3,660,651 to 3,661,169 Rv3278c Rv3279c: Rv3279c - Possible bifunctional protein BirA: biotin operon repressor + biotin--[acetyl-CoA-carboxylase] synthetase (biotin--protein ligase), at 3,661,212 to 3,662,012 Rv3279c Rv3280: Rv3280 - Probable propionyl-CoA carboxylase beta chain 5 AccD5 (pccase) (propanoyl-CoA:carbon dioxide ligase), at 3,662,062 to 3,663,708 Rv3280 Position (kb) 3660 3661 3662Strain fitness (log2 ratio) -2 -1 0 1 2at 3660.012 kb on - strand, within Rv3277at 3660.630 kb on + strandat 3660.698 kb on - strandat 3660.736 kb on + strand, within Rv3278cat 3660.736 kb on + strand, within Rv3278cat 3660.737 kb on - strand, within Rv3278cat 3660.770 kb on + strand, within Rv3278cat 3661.004 kb on + strand, within Rv3278cat 3661.004 kb on + strand, within Rv3278cat 3661.004 kb on + strand, within Rv3278cat 3661.005 kb on - strand, within Rv3278cat 3661.162 kb on + strandat 3661.181 kb on + strandat 3661.213 kb on + strandat 3662.049 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Levofloxacin 0.3 ug/mL
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3,660,012 - Rv3277 0.16 -0.9
3,660,630 + +2.4
3,660,698 - -0.9
3,660,736 + Rv3278c 0.16 +2.1
3,660,736 + Rv3278c 0.16 -1.4
3,660,737 - Rv3278c 0.17 -1.2
3,660,770 + Rv3278c 0.23 +0.3
3,661,004 + Rv3278c 0.68 -0.9
3,661,004 + Rv3278c 0.68 -1.0
3,661,004 + Rv3278c 0.68 +0.1
3,661,005 - Rv3278c 0.68 -0.8
3,661,162 + +0.7
3,661,181 + -1.1
3,661,213 + -1.0
3,662,049 - -1.8

Or see this region's nucleotide sequence