Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv2254c

Experiment: 7H9 with Levofloxacin 0.3 ug/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv2252 and Rv2253 are separated by 65 nucleotidesRv2253 and Rv2254c are separated by 32 nucleotidesRv2254c and Rv2255c are separated by 4 nucleotidesRv2255c and Rv2256c are separated by 166 nucleotides Rv2252: Rv2252 - Diacylglycerol kinase, at 2,526,989 to 2,527,918 Rv2252 Rv2253: Rv2253 - Possible secreted unknown protein, at 2,527,984 to 2,528,487 Rv2253 Rv2254c: Rv2254c - Probable integral membrane protein, at 2,528,520 to 2,528,975 Rv2254c Rv2255c: Rv2255c - Hypothetical protein, at 2,528,980 to 2,529,174 Rv2255c Rv2256c: Rv2256c - Conserved hypothetical protein, at 2,529,341 to 2,529,874 Rv2256c Position (kb) 2528 2529Strain fitness (log2 ratio) -2 -1 0 1at 2527.632 kb on - strand, within Rv2252at 2527.830 kb on - strandat 2528.155 kb on + strand, within Rv2253at 2528.173 kb on + strand, within Rv2253at 2528.174 kb on - strand, within Rv2253at 2528.174 kb on - strand, within Rv2253at 2528.347 kb on + strand, within Rv2253at 2528.348 kb on - strand, within Rv2253at 2528.348 kb on - strand, within Rv2253at 2528.439 kb on + strandat 2528.440 kb on - strandat 2528.736 kb on + strand, within Rv2254cat 2528.750 kb on - strand, within Rv2254cat 2528.981 kb on + strandat 2529.194 kb on - strandat 2529.194 kb on - strandat 2529.201 kb on - strandat 2529.243 kb on - strandat 2529.243 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Levofloxacin 0.3 ug/mL
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2,527,632 - Rv2252 0.69 -1.4
2,527,830 - -0.2
2,528,155 + Rv2253 0.34 +0.3
2,528,173 + Rv2253 0.38 -1.6
2,528,174 - Rv2253 0.38 +0.4
2,528,174 - Rv2253 0.38 -0.4
2,528,347 + Rv2253 0.72 +1.7
2,528,348 - Rv2253 0.72 -0.7
2,528,348 - Rv2253 0.72 -0.1
2,528,439 + -1.2
2,528,440 - -1.3
2,528,736 + Rv2254c 0.47 -1.7
2,528,750 - Rv2254c 0.50 -0.7
2,528,981 + -0.3
2,529,194 - -1.8
2,529,194 - -0.5
2,529,201 - -0.4
2,529,243 - -0.5
2,529,243 - -0.7

Or see this region's nucleotide sequence