Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv1824

Experiment: 7H9 with Levofloxacin 0.3 ug/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv1822 and Rv1823 overlap by 8 nucleotidesRv1823 and Rv1824 are separated by 28 nucleotidesRv1824 and Rv1825 are separated by 16 nucleotidesRv1825 and Rv1826 are separated by 37 nucleotides Rv1822: Rv1822 - Probable CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase PgsA2 (PGP synthase) (phosphatidylglycerophosphate synthase) (3-phosphatidyl-1'-glycerol-3'phosphate synthase), at 2,069,080 to 2,069,709 Rv1822 Rv1823: Rv1823 - Conserved protein, at 2,069,702 to 2,070,625 Rv1823 Rv1824: Rv1824 - Conserved hypothetical membrane protein, at 2,070,654 to 2,071,019 Rv1824 Rv1825: Rv1825 - Conserved protein, at 2,071,036 to 2,071,914 Rv1825 Rv1826: Rv1826 - Probable glycine cleavage system H protein GcvH, at 2,071,952 to 2,072,356 Rv1826 Position (kb) 2070 2071 2072Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2069.814 kb on + strand, within Rv1823at 2070.160 kb on + strand, within Rv1823at 2070.161 kb on - strand, within Rv1823at 2070.430 kb on + strand, within Rv1823at 2070.438 kb on - strand, within Rv1823at 2070.530 kb on + strand, within Rv1823at 2070.531 kb on - strand, within Rv1823at 2070.623 kb on + strandat 2070.645 kb on + strandat 2070.769 kb on - strand, within Rv1824at 2070.769 kb on - strand, within Rv1824at 2071.287 kb on - strand, within Rv1825at 2071.349 kb on + strand, within Rv1825at 2071.350 kb on - strand, within Rv1825at 2071.350 kb on - strand, within Rv1825at 2071.350 kb on - strand, within Rv1825at 2071.466 kb on + strand, within Rv1825at 2071.483 kb on - strand, within Rv1825at 2071.483 kb on - strand, within Rv1825at 2071.545 kb on - strand, within Rv1825at 2071.571 kb on + strand, within Rv1825at 2071.894 kb on + strandat 2071.894 kb on + strandat 2071.894 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Levofloxacin 0.3 ug/mL
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2,069,814 + Rv1823 0.12 +0.4
2,070,160 + Rv1823 0.50 -0.8
2,070,161 - Rv1823 0.50 -0.7
2,070,430 + Rv1823 0.79 -0.6
2,070,438 - Rv1823 0.80 -1.4
2,070,530 + Rv1823 0.90 -1.6
2,070,531 - Rv1823 0.90 +2.4
2,070,623 + -1.6
2,070,645 + -0.9
2,070,769 - Rv1824 0.31 -1.3
2,070,769 - Rv1824 0.31 -3.2
2,071,287 - Rv1825 0.29 +2.4
2,071,349 + Rv1825 0.36 -0.3
2,071,350 - Rv1825 0.36 -0.4
2,071,350 - Rv1825 0.36 +0.7
2,071,350 - Rv1825 0.36 +1.4
2,071,466 + Rv1825 0.49 -1.7
2,071,483 - Rv1825 0.51 +0.1
2,071,483 - Rv1825 0.51 +0.1
2,071,545 - Rv1825 0.58 -1.5
2,071,571 + Rv1825 0.61 -1.7
2,071,894 + -0.1
2,071,894 + -0.7
2,071,894 + -1.3

Or see this region's nucleotide sequence