Experiment: 7H9 with Levofloxacin 0.3 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv0606 and Rv0607 are separated by 53 nucleotides Rv0607 and Rv0608 are separated by 44 nucleotides Rv0608 and Rv0609 overlap by 4 nucleotides Rv0609 and Rv0609A overlap by 58 nucleotides Rv0609A and Rv0610c are separated by 694 nucleotides
Rv0606: Rv0606 - Possible transposase (fragment), at 702,016 to 702,759
Rv0606
Rv0607: Rv0607 - Hypothetical protein, at 702,813 to 703,199
Rv0607
Rv0608: Rv0608 - Possible antitoxin VapB28, at 703,244 to 703,489
Rv0608
Rv0609: Rv0609 - Possible toxin VapC28. Contains PIN domain., at 703,486 to 703,887
Rv0609
Rv0609A: Rv0609A - Conserved hypothetical protein, at 703,830 to 704,057
Rv0609A
Rv0610c: Rv0610c - Hypothetical protein, at 704,752 to 705,909
Rv0610c
Position (kb) 703
704 Strain fitness (log2 ratio) -2
-1
0
1
2 at 702.514 kb on - strand, within Rv0606 at 703.007 kb on - strand, within Rv0607 at 703.463 kb on + strand, within Rv0608 at 703.463 kb on + strand, within Rv0608 at 703.796 kb on - strand, within Rv0609 at 703.796 kb on - strand, within Rv0609 at 703.927 kb on - strand, within Rv0609A at 704.175 kb on + strand at 704.175 kb on + strand at 704.175 kb on + strand at 704.176 kb on - strand at 704.176 kb on - strand at 704.366 kb on + strand at 704.367 kb on - strand at 704.440 kb on + strand at 704.509 kb on + strand at 704.520 kb on - strand at 704.520 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Levofloxacin 0.3 ug/mL remove 702,514 - Rv0606 0.67 -0.3 703,007 - Rv0607 0.50 +2.0 703,463 + Rv0608 0.89 -1.2 703,463 + Rv0608 0.89 -0.3 703,796 - Rv0609 0.77 -2.0 703,796 - Rv0609 0.77 +0.1 703,927 - Rv0609A 0.43 -0.3 704,175 + -0.4 704,175 + -0.3 704,175 + -0.2 704,176 - -0.5 704,176 - +1.6 704,366 + +0.0 704,367 - -0.6 704,440 + -2.1 704,509 + +0.7 704,520 - -1.2 704,520 - +1.9
Or see this region's nucleotide sequence