Experiment: 7H9 with Levofloxacin 0.3 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv0221 and Rv0222 are separated by 30 nucleotides Rv0222 and Rv0223c are separated by 5 nucleotides Rv0223c and Rv0224c are separated by 98 nucleotides Rv0224c and Rv0225 are separated by 35 nucleotides
Rv0221: Rv0221 - Possible triacylglycerol synthase (diacylglycerol acyltransferase), at 264,067 to 265,476
Rv0221
Rv0222: Rv0222 - Probable enoyl-CoA hydratase EchA1 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase), at 265,507 to 266,295
Rv0222
Rv0223c: Rv0223c - Probable aldehyde dehydrogenase, at 266,301 to 267,764
Rv0223c
Rv0224c: Rv0224c - Possible methyltransferase (methylase), at 267,863 to 268,627
Rv0224c
Rv0225: Rv0225 - Possible conserved protein, at 268,663 to 269,817
Rv0225
Position (kb) 266
267
268 Strain fitness (log2 ratio) -2
-1
0
1 at 265.315 kb on + strand, within Rv0221 at 265.316 kb on - strand, within Rv0221 at 265.507 kb on - strand at 265.832 kb on - strand, within Rv0222 at 265.976 kb on - strand, within Rv0222 at 266.016 kb on - strand, within Rv0222 at 266.016 kb on - strand, within Rv0222 at 266.152 kb on - strand, within Rv0222 at 266.184 kb on + strand, within Rv0222 at 266.449 kb on + strand, within Rv0223c at 266.527 kb on + strand, within Rv0223c at 266.573 kb on - strand, within Rv0223c at 266.660 kb on + strand, within Rv0223c at 266.740 kb on + strand, within Rv0223c at 266.740 kb on + strand, within Rv0223c at 267.006 kb on + strand, within Rv0223c at 267.080 kb on + strand, within Rv0223c at 267.409 kb on + strand, within Rv0223c at 267.409 kb on + strand, within Rv0223c at 267.658 kb on + strand at 267.659 kb on - strand at 267.682 kb on - strand at 267.764 kb on + strand at 267.764 kb on + strand at 267.765 kb on - strand at 267.765 kb on - strand at 267.865 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Levofloxacin 0.3 ug/mL remove 265,315 + Rv0221 0.89 -0.9 265,316 - Rv0221 0.89 -0.3 265,507 - -0.2 265,832 - Rv0222 0.41 -1.0 265,976 - Rv0222 0.59 -0.7 266,016 - Rv0222 0.65 -0.6 266,016 - Rv0222 0.65 -0.0 266,152 - Rv0222 0.82 -1.1 266,184 + Rv0222 0.86 -1.4 266,449 + Rv0223c 0.10 -0.8 266,527 + Rv0223c 0.15 -1.8 266,573 - Rv0223c 0.19 -0.8 266,660 + Rv0223c 0.25 -1.5 266,740 + Rv0223c 0.30 -0.8 266,740 + Rv0223c 0.30 +1.2 267,006 + Rv0223c 0.48 +0.2 267,080 + Rv0223c 0.53 +0.4 267,409 + Rv0223c 0.76 -0.6 267,409 + Rv0223c 0.76 -1.5 267,658 + -0.6 267,659 - +0.8 267,682 - +0.2 267,764 + +0.1 267,764 + +0.5 267,765 - -0.9 267,765 - -0.4 267,865 - -0.4
Or see this region's nucleotide sequence