Experiment: 7H9 with Levofloxacin 0.3 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv0214 and Rv0215c are separated by 105 nucleotides Rv0215c and Rv0216 are separated by 56 nucleotides
Rv0214: Rv0214 - Probable fatty-acid-CoA ligase FadD4 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase), at 256,064 to 257,677
Rv0214
Rv0215c: Rv0215c - Probable acyl-CoA dehydrogenase FadE3, at 257,783 to 258,856
Rv0215c
Rv0216: Rv0216 - Double hotdog hydratase, at 258,913 to 259,926
Rv0216
Position (kb) 257
258
259 Strain fitness (log2 ratio) -1
0
1
2 at 257.069 kb on + strand, within Rv0214 at 257.220 kb on - strand, within Rv0214 at 257.247 kb on - strand, within Rv0214 at 257.559 kb on + strand at 257.643 kb on - strand at 257.720 kb on + strand at 257.721 kb on - strand at 257.753 kb on - strand at 257.803 kb on - strand at 257.926 kb on - strand, within Rv0215c at 257.991 kb on + strand, within Rv0215c at 257.992 kb on - strand, within Rv0215c at 258.531 kb on + strand, within Rv0215c at 258.532 kb on - strand, within Rv0215c at 258.532 kb on - strand, within Rv0215c at 258.532 kb on - strand, within Rv0215c at 258.651 kb on + strand, within Rv0215c at 258.681 kb on + strand, within Rv0215c at 258.747 kb on + strand, within Rv0215c at 258.751 kb on + strand at 258.751 kb on + strand at 258.751 kb on + strand at 258.752 kb on - strand at 258.752 kb on - strand at 259.193 kb on - strand, within Rv0216
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Levofloxacin 0.3 ug/mL remove 257,069 + Rv0214 0.62 -0.5 257,220 - Rv0214 0.72 -0.5 257,247 - Rv0214 0.73 +0.0 257,559 + -0.7 257,643 - -1.5 257,720 + -0.7 257,721 - -0.3 257,753 - -0.4 257,803 - +0.4 257,926 - Rv0215c 0.13 -1.0 257,991 + Rv0215c 0.19 +0.3 257,992 - Rv0215c 0.19 -0.1 258,531 + Rv0215c 0.70 +0.1 258,532 - Rv0215c 0.70 +0.1 258,532 - Rv0215c 0.70 +2.0 258,532 - Rv0215c 0.70 -0.3 258,651 + Rv0215c 0.81 -0.4 258,681 + Rv0215c 0.84 -1.0 258,747 + Rv0215c 0.90 +1.5 258,751 + +0.0 258,751 + -0.0 258,751 + +1.0 258,752 - +0.1 258,752 - -0.1 259,193 - Rv0216 0.28 -1.0
Or see this region's nucleotide sequence