Experiment: 7H9 with Levofloxacin 0.3 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv0147 and Rv0148 are separated by 74 nucleotides Rv0148 and Rv0149 are separated by 6 nucleotides Rv0149 and Rv0150c overlap by 4 nucleotides Rv0150c and Rv0151c are separated by 590 nucleotides
Rv0147: Rv0147 - Probable aldehyde dehydrogenase (NAD+) dependent, at 173,238 to 174,758
Rv0147
Rv0148: Rv0148 - Probable short-chain type dehydrogenase/reductase, at 174,833 to 175,693
Rv0148
Rv0149: Rv0149 - Possible quinone oxidoreductase (NADPH:quinone oxidoreductase) (zeta-crystallin), at 175,700 to 176,668
Rv0149
Rv0150c: Rv0150c - Conserved hypothetical protein, at 176,665 to 176,952
Rv0150c
Rv0151c: Rv0151c - PE family protein PE1, at 177,543 to 179,309
Rv0151c
Position (kb) 175
176
177 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 174.798 kb on + strand at 174.798 kb on + strand at 174.799 kb on - strand at 174.799 kb on - strand at 174.896 kb on + strand at 175.192 kb on + strand, within Rv0148 at 175.205 kb on + strand, within Rv0148 at 175.214 kb on + strand, within Rv0148 at 175.224 kb on + strand, within Rv0148 at 175.225 kb on - strand, within Rv0148 at 175.246 kb on - strand, within Rv0148 at 175.322 kb on + strand, within Rv0148 at 175.323 kb on - strand, within Rv0148 at 175.323 kb on - strand, within Rv0148 at 175.323 kb on - strand, within Rv0148 at 175.323 kb on - strand, within Rv0148 at 175.323 kb on - strand, within Rv0148 at 175.323 kb on - strand, within Rv0148 at 175.386 kb on - strand, within Rv0148 at 175.386 kb on - strand, within Rv0148 at 175.386 kb on - strand, within Rv0148 at 175.502 kb on + strand, within Rv0148 at 175.502 kb on + strand, within Rv0148 at 175.620 kb on + strand at 175.621 kb on - strand at 175.692 kb on - strand at 175.697 kb on + strand at 175.717 kb on - strand at 175.748 kb on + strand at 175.767 kb on + strand at 175.850 kb on + strand, within Rv0149 at 175.850 kb on + strand, within Rv0149 at 175.851 kb on - strand, within Rv0149 at 176.066 kb on + strand, within Rv0149 at 176.067 kb on - strand, within Rv0149 at 176.078 kb on + strand, within Rv0149 at 176.078 kb on + strand, within Rv0149 at 176.235 kb on - strand, within Rv0149 at 176.235 kb on - strand, within Rv0149 at 176.309 kb on + strand, within Rv0149 at 176.309 kb on + strand, within Rv0149 at 176.310 kb on - strand, within Rv0149 at 176.310 kb on - strand, within Rv0149 at 176.397 kb on + strand, within Rv0149 at 176.398 kb on - strand, within Rv0149 at 176.428 kb on - strand, within Rv0149 at 176.428 kb on - strand, within Rv0149 at 176.465 kb on - strand, within Rv0149 at 176.465 kb on - strand, within Rv0149 at 176.465 kb on - strand, within Rv0149 at 176.691 kb on + strand at 177.046 kb on + strand at 177.046 kb on + strand at 177.046 kb on + strand at 177.046 kb on + strand at 177.047 kb on - strand at 177.047 kb on - strand at 177.179 kb on + strand at 177.180 kb on - strand at 177.344 kb on - strand at 177.511 kb on + strand at 177.511 kb on + strand at 177.511 kb on + strand at 177.511 kb on + strand at 177.511 kb on + strand at 177.511 kb on + strand at 177.514 kb on + strand at 177.515 kb on - strand at 177.515 kb on - strand at 177.515 kb on - strand at 177.515 kb on - strand at 177.515 kb on - strand at 177.572 kb on + strand at 177.573 kb on - strand at 177.573 kb on - strand at 177.573 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Levofloxacin 0.3 ug/mL remove 174,798 + -1.3 174,798 + -0.3 174,799 - -1.1 174,799 - +0.6 174,896 + -1.1 175,192 + Rv0148 0.42 +0.5 175,205 + Rv0148 0.43 -0.3 175,214 + Rv0148 0.44 +1.3 175,224 + Rv0148 0.45 -1.2 175,225 - Rv0148 0.46 -0.4 175,246 - Rv0148 0.48 -0.6 175,322 + Rv0148 0.57 +1.5 175,323 - Rv0148 0.57 +0.1 175,323 - Rv0148 0.57 +1.8 175,323 - Rv0148 0.57 -1.2 175,323 - Rv0148 0.57 -1.7 175,323 - Rv0148 0.57 -1.4 175,323 - Rv0148 0.57 -2.6 175,386 - Rv0148 0.64 +1.1 175,386 - Rv0148 0.64 +0.3 175,386 - Rv0148 0.64 -1.9 175,502 + Rv0148 0.78 -1.1 175,502 + Rv0148 0.78 +1.7 175,620 + -1.6 175,621 - -1.2 175,692 - +0.8 175,697 + -0.2 175,717 - -0.4 175,748 + -1.4 175,767 + -0.8 175,850 + Rv0149 0.15 -0.5 175,850 + Rv0149 0.15 +0.2 175,851 - Rv0149 0.16 -0.3 176,066 + Rv0149 0.38 +2.3 176,067 - Rv0149 0.38 +2.9 176,078 + Rv0149 0.39 -0.4 176,078 + Rv0149 0.39 +0.7 176,235 - Rv0149 0.55 -0.4 176,235 - Rv0149 0.55 +0.4 176,309 + Rv0149 0.63 -1.2 176,309 + Rv0149 0.63 -0.4 176,310 - Rv0149 0.63 -0.9 176,310 - Rv0149 0.63 -0.4 176,397 + Rv0149 0.72 -1.0 176,398 - Rv0149 0.72 -0.2 176,428 - Rv0149 0.75 +0.4 176,428 - Rv0149 0.75 -1.5 176,465 - Rv0149 0.79 +0.1 176,465 - Rv0149 0.79 -1.5 176,465 - Rv0149 0.79 -0.2 176,691 + -2.4 177,046 + -1.0 177,046 + -0.6 177,046 + -0.3 177,046 + -0.3 177,047 - +1.9 177,047 - -0.7 177,179 + +1.6 177,180 - -0.7 177,344 - +1.4 177,511 + -0.5 177,511 + -0.1 177,511 + -0.7 177,511 + -1.1 177,511 + +0.5 177,511 + -0.7 177,514 + +2.9 177,515 - +0.9 177,515 - +2.9 177,515 - +0.2 177,515 - -1.1 177,515 - +0.2 177,572 + -1.4 177,573 - -0.5 177,573 - -1.5 177,573 - +0.4
Or see this region's nucleotide sequence